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Journal : bionature

BIOPROSES PENGURAIAN LIMBAH CAIR BUANGAN INDUSTRI PANGAN DAN NON-PANGAN BERBASIS BIOSURFAKTAN Yati Sudaryati Soeka; Joko Sulistyo
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.35848

Abstract

Biosurfactants are microbial metabolites that may be utilized to replace synthetic chemical surfactant in overcoming the problem of industrial wastewater pollution. Indications of the presence of biosurfactant activity may be determined through measurement of emulsification and enzymatic activity of the tested microorganisms on their growth media, which is indicated by decreasing the BOD and COD of the tested growth media containing wastewater material as an inducer added to the microbial growth medium. This research was conducted to examine several potential microbial isolates that produce biosurfactants on growth media containing mixed waste water. The results showed that some of the microbial cultures tested showed the potential to produce biosurfactants as indicated by the emulsification and enzymatic activity of the tested microbial culture. Trial on mixed industrial wastewater showed that the tested biosurfactants were able to reduce the levels of BOD (440-1300 mg/l) and COD (1240-1760 mg/l) of the wastewater samples up to low BOD levels (82 mg/l) and COD (116 mg/l), respectively, thus that it meets the requirements of the established wastewater quality standards according to the Indonesian Standard regarding with the respective BOD (50-100 mg/l, 06-6989.9-2004 SNI) and COD (100-250 mg /l, 06-6989.2-2004 SNI).Key words : biosurfactant, emulsification, microbial enzyme, wastewater, BOD, COD.
Some Properties of Oyster Mushroom Grown on Substrates Containing Paper Waste Joko Sulistyo
bionature Vol 24, No 2 (2023): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i2.45548

Abstract

ABSTRACTThis study was conducted to determine the activity of starter culture of oyster mushroom and influence of substrates containing paper waste materials used to grow the mushroom. The purpose of the study was to analyze nutritional value and antioxidant activity of the mushroom grown on the substrate contained paper waste. The substrate was prepared using paper waste with 5 different formulations were 0% (control), 20%, 30%, 40%and 50% paper waste. Proximate analysis, heavy metal content and antioxidant activity were carried out to test the quality of the mushroom. Overall, the mushroom grown on the substrates containing paper waste showed high water content, however, the mushroom grown on the substrate contained 50% paper waste showed the highest content of ash, protein, and crude fiber, while higher fat content showed by the mushroom grown on the substrate contained 50% paper waste compared to the mushroom grown on other substrate with different composition of paper waste. The mushroom grown on the substrate contained 50% paper waste indicated considerably high content of heavy metals such as lead, (3.5%), copper (20.8%) and cadmium (4.4%), respectively however, it showed the higher antioxidant activity (87.19%) as well compared to the mushrooms grown on the substrate containing a lower percentage of paper waste, thus giving a conclusion that there was probably positive correlation in between the heavy metals contained in paper waste with antioxidant activity. Keywords: paper waste, oyster mushroom, proximate analysis, antioxidant, heavy metals. ABSTRAKPenelitian ini dilakukan untuk menentukan aktivitas kultur starter jamur tiram dan menguji substrat yang mengandung limbah kertas yang digunakan untuk menumbuhkan jamur. Tujuan penelitian adalah untuk menganalisis nilai gizi dan aktivitas antioksidan dari jamur yang ditumbuhkan pada substrat mengandung limbah kertas. Substrat disiapkan menggunakan limbah kertas dengan 5 formula berbeda yaitu 0% (control), 20%, 30%, 40% dan 50%. Analisis proksimat, kandungan logam berat dan aktivitas antioksidan dilakukan untuk menguji kualitas dari jamur. Secara keseluruhan, jamur tiram yang ditumbuhkan pada substrat limbah kertas menunjukkan kadar air yang tinggi, namun jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kadar abu, protein, dan serat kasar yang tertinggi, sementara kadar lemak pada jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan lebih tinggi dibandingkan dengan jamur yang ditumbuhkan pada substrat dengan komposisi limbah kertas lainnya. Jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kandungan logam berat, masing-masing seperti timbal (3.5%), tembaga (20.8%) dan kadmium (4.4%) yang cukup tinggi, namun juga menunjukkan aktivitas antioksidan yang lebih tinggi (87.19%) dibandingkan dengan jamur yang ditumbuhkan pada substrat mengandung persentase limbah kertas yang lebih rendah, sehingga memberikan kesimpulan bahwa terdapat korelasi yang positif antara logam berat yang terkandung dalam limbah kertas dengan aktivitas antioksidan.Kata kunci: limbah kertas, jamur tiram, nilai gizi, analisis proksimat. Antioksidan, logam berat.