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All Journal HAYATI Journal of Biosciences The Indonesian Biomedical Journal Jurnal Medik Veteriner Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika)

Wardaya Wardaya

Doctoral Program In Biomedical Sciences, Faculty Of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. Indonesian Air Force Institute Of Aviation Medicine (Lembaga Kesehatan Penerbangan Dan Ruang Angkasa Dr. Saryanto), Jakarta 12770, Indonesi

Author-ID : 6622973

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

Published : 6 Documents Claim Missing Document

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Articles

Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure Margaretha Herawati; . Wardaya; Wawan Mulyawan; Fanny Septiani Farhan; Frans Ferdinal; Sri Widia A. Jusman; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.

Analisis Respons Stres Oksidatif, Antioksidan, dan Adaptasi Fisiologis Jaringan Otak Pasca Induksi Hipoksia Hipobarik Intermiten Pada Tikus Spraque Dowley Wardaya Wardaya; Marjani Khoirunnisa; Asep Edi Sukmayadi
Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika) Vol 4 No 2 (2018): Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika)
Publisher : Politeknik Kesehatan TNI AU Ciumbuleuit Bandung

Penurunan konsentrasi oksigen dapat terjadi pada kondisi fisiologis tubuh maupun patologis. Hipoksia merupakan salah satu faktor yang berkontribusi dalam patomekanisme serangan jantung, stroke, hipertensi pulmonal dan penyakit-penyakit lain yang dapat menyebabkan kematian. Dasar ilmiah dari latihan hipoksia hipobarik intermiten pada Hypobaric chamber training ini, terutama mengenai manfaatnya, hingga saat ini masih belum banyak dipublikasikan. Tujuan penelitian ini adalah untuk menganalisis respons stres oksidatif, antioksidan, dan adaptasi fisiologis jaringan otak pasca induksi hipoksia hipobarik intermiten pada tikus Spraque dowley. Penelitian ini merupakan penelitian eksperimental in vivo dengan menggunakan hewan coba. Kegiatan penelitian ini dilaksanakan selama 5 (lima) bulan yaitu mulai bulan Agustus sampai dengan Desember. Tempat kegiatan penelitian di Laboratorium Aerofisiologi Lakespra Saryanto dan Laboratorium Biokimia Biomolekuler FKUI Salemba. Penelitian yang telah dilakukan menunjukkan bahwa pada pemeriksaan kadar MDA pada otak tikus didapatkan hasil peningkatan kadar MDA pada kelompok hari ke-21 yaitu tikus yang mengalami tiga kali pemaparan hipoksia hipobarik walaupun tidak bermakna, sedangkan pada kelompok tikus yang mendapat paparan ulang hipoksia hipobarik (kelompok hari ke-7, 14 dan 28), didapatkan kadar MDA yang lebih rendah secara signifikan dibandingkan kontrol (p<0,005).

Pengaruh Pemberian Vitamin C Terhadap Kadar Asam Urat Dan Superoxide Dismutase Di Ketinggian 25.000 Kaki Pada Siswa Susjursartakes Wardaya Wardaya; Mintoro Sumego; Desi Sundari Utami
Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika) Vol 5 No 2 (2019): Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika)
Publisher : Politeknik Kesehatan TNI AU Ciumbuleuit Bandung

Untuk dapat meningkatkan kesehatan fisik maupun mental hendaklah disadari bagi setiap awak untuk menghindari dari terpaparnya berbagai macam penyakit. Salah satu hal yang dapat mengganggu kinerja adalah meningkatnya kadar asam urat (hiperuresemia) yang dapat diketahui lewat pemeriksaan laboratorium. Superoxide dismutase (SOD) merupakan enzim pertama dalam mekanisme per-tahanan terhadap superoksida dan dapat mengurangi reaksi superoksida yang kemudian melindungi tubuh dari keracunan suproksida, namun heat stress diketahui dapat menyebabkan penurunan kadar SOD. Tujuan penelitian ini adalah Untuk mengetahui apakah terdapat pengaruh antara pemberian antioksidan Vitamin C terhadap Kadar Asam Urat dan Superoxide Dismutase pada siswa Sejursartakes A 27 Skadik 504 di Lakespra Saryanto. Penelitian ini merupakan penelitian eksperimental in vivo dengan design analisis data T test berpasangan, karena data yang didapatkan merupakan data numerik. Probandus pada penelitian ini adalah manusia, dengan dibagi dalam dua kelompok, yaitu kelompok I yg tidak mendapatkan perlakuan dan kelompok II yang mendapatkan perlakuan berupa pemberian vitamin C. Penelitian dilaksanakan di Ruangan Udara Bertekanan Rendah (RUBR) Lakespra Saryanto dan Biokimia Biomolekuler FKUI. Sampel diambil dengan total sampel sebanyak 23 orang. Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa terdapat pengaruh bermakna pada pemberian vitamin C terhadap kadar Asam Urat, tetapi tidak bermakna pada SOD walaupun terjadi peningkatan pada Siswa Sejursartakes A 27 Skadik 504 tahun 2019.

Oxidative Stress, Hypoxia-Inducible Factor-1α, and Nuclear Factor-Erythroid 2-Related Factor 2 in the Hearts of Rats Exposed to Intermittent Hypobaric Hypoxia Wardaya Wardaya; Wawan Mulyawan; Sri Widia A Jusman; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.39-47

Hypobaric hypoxia is situation that might occur to helicopter pilots in Indonesia who must fly at an altitude of more than 3,048 m such as in Papua. It can be dangerous because hypoxic condition can affect person's performance. So far, the heart is known as an aerobic organ and very sensitive to hypoxic conditions. Hitherto, the effects of hypobaric hypoxia exposure on biomolecular aspects of the heart are still unclear. Therefore, this study assessed cardiac response in rats exposed to intermittent hypobaric hypoxia (IHH) (equivalent to 3,048 meters/10,000 feet). Sprague-Dawley rats were divided into six groups: control; acute hypobaric hypoxia (AHH); and IHH, for 7; 14; 21; and 28 days. We measured super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH), malondialdehyde (MDA), cytoglobin, myoglobin, HIF-1α, and Nrf2 level as our parameters. Activities of SOD, CAT, GPx and GSH increased while the levels of MDA, cytoglobin, myoglobin, HIF-1α, and Nrf2 decreased in all IHH groups compared with the AHH group. A biphasic pattern was observed as IHH sessions increased from 14 to 21 or 28. Where the IHH treatment for more than 14 sessions caused a decrease in endogenous antioxidants, but the response to hypoxia and oxidative stress increased. Our findings presented the molecular alterations of cardiac rats exposed to intermittent hypobaric hypoxia.

Intermittent Exposure to Hypobaric Hypoxia Increases VEGF, HIF-1α, and Nrf-2 Expressions in Brain Tissue Wardaya Wardaya; Dewi Sukmawati; Nurhadi Ibrahim; Frans Ferdinal; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A. Jusman; Irawan Satriotomo; Wawan Mulyawan
The Indonesian Biomedical Journal Vol 17, No 2 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i2.3519

BACKGROUND: Hypoxia inducible factor-1α (HIF-1α), nuclear factor erythroid 2-related factor 2 (Nrf-2), and vascular endothelial growth factor (VEGF), play a crucial role as neuroprotective factors. Currently, there is a lack of studies examining the biomolecular responses of the brain to intermittent hypoxia resulting from various pressures. This study was conducted to investigate the physiological responses, histopathological features, and cellular adaptive responses in the brains of rats that were intermittently exposed to hypobaric hypoxic conditions.METHODS: Thirty male Sprague Dawley rats were divided into six groups: a control group and five treatment groups exposed to hypobaric hypoxia. The treatment groups were placed in a hypobaric chamber simulating an altitude of 3,048 meters for 1 hour/day for 1, 7, 14, 21, and 28 days. After exposure, brain tissue was collected for histopathological analysis and protein quantification of HIF-1α, Nrf-2, cytoglobin (Cygb), neuroglobin (Ngb), VEGF, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT).RESULTS: In the brain, intermittent hypobaric hypoxia significantly increased HIF-1α expression (p=0.000) and its downstream proteins Cygb (p=0.000), and VEGF (p=0.001), with a peak at 14x IHH exposure compared to control. This was followed by a significant increase in Nrf-2 expression (p=0.000), SOD (p=0.000), Gpx (p=0.000), and CAT activity (p=0.000), indicating an adaptive antioxidant response. Conversely, MDA levels was decreased with prolonged exposure, suggesting reduced oxidative damage.CONCLUSION: IHH elevates HIF-1α, Nrf-2, and oxidative stress markers, triggering an adaptive antioxidant response in the rat’s brains.KEYWORDS: HIF-1α, intermittent hypobaric hypoxia, Nrf-2, oxidative stress

Intermittent hypobaric hypoxia induces oxidative stress and vascular remodeling in rat pulmonary arteries Ardi, Sri Nurbowo; Putra, Muhammad Arza; Sukmawati, Dewi; Wuyung, Puspita Eka; Mulyawan, Wawan; Wardaya; Wiyono, Wiwien Heru
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.29-40

Flight crews frequently experience intermittent hypobaric hypoxia (IHH), which may trigger oxidative stress and vascular remodeling in the pulmonary arteries. This study investigated the effects of IHH exposure on oxidative stress markers, tunica media thickness, and the expression of LR11 and HIF-1α proteins in rat pulmonary arteries using five groups of healthy male Sprague–Dawley rats (n = 5 per group). Twenty-five male Sprague–Dawley rats were divided into five groups: a control group and four treatment groups exposed to simulated altitudes of 25,000 ft (7,620 m) for 5 minutes per day over 1, 3, 7, or 14 days. Levels of HIF-1α and LR11 were measured using ELISA, oxidative stress markers such as γ-glutamyl-cysteinyl-glycine (GSH) and malondialdehyde (MDA) were analyzed spectrophotometrically, and tunica media thickness was assessed histologically. HIF-1α levels peaked after acute hypoxia exposure and declined with prolonged exposure, whereas LR11 levels progressively increased, peaking in the 14-day group. MDA levels, reflecting oxidative stress, were highest after 3 days of exposure, while GSH levels decreased in all hypoxia groups compared with the control. Histological analysis showed the greatest tunica media thickness in the 3-day exposure group, followed by gradual reductions. Although no statistically significant correlations were found between protein levels and tunica media thickness, similar directional trends suggest biologically relevant associations. Intermittent hypobaric hypoxia (IHH) induces dynamic changes in oxidative stress and vascular remodeling in rat pulmonary arteries. Acute hypoxia increases HIF-1α expression and oxidative stress, whereas repeated exposures appear to trigger compensatory adaptations, including LR11 upregulation and tunica media remodeling. These findings demonstrate measurable molecular and structural alterations underlying pulmonary vascular responses to repeated high-altitude exposure.

Co-Authors Ardi, Sri Nurbowo Asep Edi Sukmayadi Desi Sundari Utami Dewi Sukmawati Dewi Sukmawati Fanny Septiani Farhan Frans Ferdinal Frans Ferdinal Irawan Satriotomo Khoirunnisa, Marjani Mintoro Sumego Mohamad Sadikin Mohamad Sadikin Mohamad Sadikin Ninik Mudjihartini Nurhadi Ibrahim Puspita Eka Wuyung Putra, Muhammad Arza Sri Widia A Jusman Sri Widia A. Jusman Sri Widia A. Jusman Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wawan Mulyawan Wiwien Heru Wiyono

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