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All Journal ACTA VETERINARIA INDONESIANA International Journal of Integrated Health Sciences Althea Medical Journal Indonesian Journal of Cancer Chemoprevention Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat The Indonesian Biomedical Journal Oto Rhino Laryngologica Indonesiana

Gita Widya Pradini

Departemen Ilmu Kedokteran Dasar, Fakultas Kedokteran Universitas Padjadjaran

Author-ID : 663942

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Engineering Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Veterinary

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Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm Arifani, Ilma; Pradini, Gita Widya; Desy Arya, Insi Farisa; Cahyadi, Adi Imam
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Background: Pseudomonas aeruginosa is the most common bacteria contaminating the hemodialysis water and has high capability to form a biofilm. The presence of biofilm is hazardous because it becomes a constant source of bacterial and toxin release toward the hemodialysis patientâs blood. Calcium hypochlorite (Ca(OCl)2) is an easily obtained disinfectant. This study was aimed to detect the destructive effect of Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration required to achieve significant effect.Methods: This experimental study was conducted in six replicates from September to October 2015 in Microbiology Laboratory of Faculty of Medicine Universitas Padjadjaran Bandung. A modified tissue culture plate method was performed to grow P. aeruginosa biofilms which were subsequently treated with Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts per million (ppm). The data was analyzed using Welch Analysis of Variance (ANOVA) and Games-Howell post-hoc tests and presented in tables.Results: Data were obtained from 36 flat-bottomed polystyrene wells. There was a statistically significant mean difference between groups [F(4, 11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and p< 0.001 for 30, 40, and 500 ppm).Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500 ppm have significant destructive effect against P. aeruginosa biofilm. The mean differences among treated groups were not significant. The most optimum concentration is 30 ppm.

Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm Ilma Arifani; Gita Widya Pradini; Insi Farisa Desy Arya; Adi Imam Cahyadi
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Pseudomonas aeruginosa is the most common bacteria contaminating the hemodialysis water and has high capability to form a biofilm. The presence of biofilm is hazardous because it becomes a constant source of bacterial and toxin release toward the hemodialysis patient’s blood. Calcium hypochlorite (Ca(OCl)2) is an easily obtained disinfectant. This study was aimed to detect the destructive effect of Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration required to achieve significant effect.Methods: This experimental study was conducted in six replicates from September to October 2015 in Microbiology Laboratory of Faculty of Medicine Universitas Padjadjaran Bandung. A modified tissue culture plate method was performed to grow P. aeruginosa biofilms which were subsequently treated with Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts per million (ppm). The data was analyzed using Welch Analysis of Variance (ANOVA) and Games-Howell post-hoc tests and presented in tables.Results: Data were obtained from 36 flat-bottomed polystyrene wells. There was a statistically significant mean difference between groups [F(4, 11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and p< 0.001 for 30, 40, and 500 ppm).Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500 ppm have significant destructive effect against P. aeruginosa biofilm. The mean differences among treated groups were not significant. The most optimum concentration is 30 ppm.

Presence of Multidrug Resistance (MDR) Escherichia coli in the Citarum River located in Greater Bandung Area, Indonesia Megantara, Imam; Muhtado, Azmi Raffi; Pradini, Gita Widya; Goenawan, Hanna; Sylviana, Nova
Althea Medical Journal Vol 10, No 3 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n3.2765

Background: One of the main problems of the Citarum River is the contamination of E. coli due to livestock activities, washing toilets, and industry. In addition, irrational use of antibiotics in the community and livestock can increase E. coli resistant strains to antibiotics. This study aimed to identify the presence of multidrug resistance (MDR) and extended-spectrum β-lactamase (ESBL) E. coli strains in Citarum river clusters, namely industrial, livestock, and residential clusters.Methods: This was a descriptive study. A sample of 100 mL surface water from each Citarum cluster. Culture, antibiotic sensitivity test, and PCR to identify blaCTX-M-15 gene carriers of ESBL E. coli were carried out in the sample.Results: There were 37 isolates of E. coli, with 24% of these isolates showing MDR properties, which can be found in industrial, livestock, and residential clusters at 13%, 8%, and 3% respectively. The most E. coli-resistant antibiotics found in these samples were ampicillin (45%), followed by tetracycline (37%), and azithromycin (29%). The PCR examination did not find the blaCTX-M-15 gene carrying ESBL properties in all three Citarum river clusters.Conclusion: The presence of E. coli isolates in each Citarum river cluster suggests the occurrence of river pollution due to animal, human or industrial waste. Therefore, it is necessary to make better government regulations regarding sanitation and education for the surrounding community regarding the importance of keeping the river clean.

Co-Authors Adi Imam Cahyadi Arifani, Ilma Bashari, Muhammad Hasan Chrysanti Murad Damara, Fachreza Aryo Edhyana Sahiratmadja Edhyana Sahiratmadja Eko Fuji Ariyanto Fauziah, Nisa Gatot Nyarumenteng Adhipurnawan Winarno Hanna Goenawan Hardani, Isna Nisrina Herman Susanto Herman Susanto Huriyah Hafizhotul 'Ummah Ika Agus Rini Ika Agus Rini Ilma Arifani Imam Megantara Insi Farisa Desy Arya Mas Rizky A.A. Syamsunarno Melati Sudiro Muhammad Ersyad Hamda Muhammad Fadhil Ihsan Yazid Muhtado, Azmi Raffi Mutia Latief Nova Sylviana Putri, Tenny Reiva Farah Dwiyana Rini Widyastuti Roostita L Balia Salsabilla Fasya Khoerunnisa Vera Amalia Lestari

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