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All Journal Public Health of Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal of Pharmaceutical and Sciences.
Ikhsan Hi. Amir Sene
Akademi Farmasi Tadulako Farma Palu, Kota Palu, Sulawesi Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Identifikasi DNA babi sebagai metode validasi untuk autentikasi halal menggunakan polymerase chain reaction Mariyani Mariyani; Ni nyoman Yuvita Yani; Ikhsan Hi. Amir Sene
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.296

Abstract

To protect consumers from fake halal labelling on food products, cosmetics and medicines, a method is needed to guarantee a product's Halal. Pork is one type that is often used to mix with beef because the two types of meat have physical similarities if not carefully considered. DNA-based analysis, often used for halal authentication, is the real-time PCR method, so this study aims to prove that conventional PCR methods can detect DNA at the same concentration. This study uses two parameters: the specificity test carried out using pig DNA samples and cow and chicken DNA as a comparison. The second parameter is the detection limit test on absolute DNA carried out at 4 concentrations, namely 50, 5, 0.5 and 0.05 ng/µL, while the relative detection limit test (pork-cow mixture) with variations in pork concentration, namely 100%, 5%, 3%, 1%, and 0.5%. The analysis showed that the primers were specific to pig DNA with an absolute detection limit of 0.05 ng/µL and a relative detection limit of 0.5%. This PCR method meets the validation requirements for identifying target species so that it can be used for halal authentication of various products.
The Potency of Typical Plants of Central Sulawesi Province to Prevent Iron Deficiency Anemia: Experimental Study Mulia Sakti, Putri; Hi. Amir Sene, Ikhsan; Ramadhan, Kadar
Public Health of Indonesia Vol. 10 No. 1 (2024): January - March
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v10i1.775

Abstract

Background: Central Sulawesi falls within the Wallacea region, making it home to various endemic flora and fauna. One distinctive plant in Central Sulawesi is the Moringa tree. The Moringa tree, especially its leaves, was often used as a culinary ingredient known as sayurkelor in the traditional cuisine of the Kaili ethnic group in Central Sulawesi Province. Objective: This research aimed to determine the effectiveness of moringa leaf extract in increasing hemoglobin levels and erythrocyte count. Method: Twenty-four male Wistar rats strain (Rattusnorvegicus) 9−10 weeks old and 200−250 grams were divided into four groups (n=6), normal as a no treatment, negative control, ethanol extract, and positive control during the 15thday. Blood samples on the 15thday were administered to determine blood count. Result: The resultsof this study showed differences in hemoglobin and erythrocyte levels in the four experimental groups.The significant difference in average hemoglobin levels between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is significant with a P-value <0.05 (P value 0.00), and the difference between Group 3 and Group 4 is significant with a P-value <0.05 (P value 0.03). the significant difference in the average erythrocyte counts between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is not significant with a P-value >0.05 (P value 0.07). The difference between Group 3 and Group 4 has a P-value >0.05 (P value 0.63). Conclusion: The research results indicate that pregnant Wistar rats experienced hemodilution characterized by a decrease in hemoglobin and erythrocyte levels during pregnancy. However, after being given ethanol extract from moringa leaves, there was an increase in hemoglobin and erythrocyte levels. Keywords: Extract; ethanol; moringa; oleifera; hemoglobin
Analysis Pork Contamination in Beef Meatballs through Polymerase Chain Reaction in Palu City Tresya J. Polibaon; Mariyani; Joni Tandi; Tien Wahyu Handayani; Rahmawati Salsa Dinurrosifa; Ikhsan Hi. Amir Sene
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.9006

Abstract

The need for halal products is essential, particularly among various processed beef products like meatballs, which are popular among nearly the entire community. These products are susceptible to issues of meat counterfeiting for greater economic gain. This study aims to determine the smallest DNA concentration that can still be detected and to assess whether there is any pork contamination in meatballs circulating in Palu City using Polymerase Chain Reaction (PCR). This test uses four DNA samples obtained from fresh meat to test the specificity of the primer: wild boar, rat, and goat DNA samples as comparisons, and pig DNA as a positive control. The presence of DNA bands in the agarose gel electrophoresis visualization results indicates that DNA amplification has occurred, meaning that the sample can be detected. The analysis results showed that the primer used (Cyt-b) had good specificity for detecting pork DNA, while the absolute pork DNA concentration of 0.01 ng/µL and the reference meatball DNA with a concentration of 0.01% are the smallest limits that can still be detected using PCR. Of the four meatball samples from Palu City, one sample produced a DNA band, indicating that the sample contains or is contaminated with pig DNA. This method is valid as it meets the specificity test and demonstrates a good detection limit.
Co-Authors Joni Tandi Kadar Ramadhan Mariyani Ni nyoman Yuvita Yani Putri Mulia Sakti Rahmawati Salsa Dinurrosifa Tien Wahyu Handayani Tresya J. Polibaon