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All Journal Public Health of Indonesia Journal of Pharmaceutical and Sciences. Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan
Ikhsan Hi. Amir Sene
Akademi Farmasi Tadulako Farma Palu, Kota Palu, Sulawesi Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Identifikasi DNA babi sebagai metode validasi untuk autentikasi halal menggunakan polymerase chain reaction Mariyani Mariyani; Ni nyoman Yuvita Yani; Ikhsan Hi. Amir Sene
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.296

Abstract

To protect consumers from fake halal labelling on food products, cosmetics and medicines, a method is needed to guarantee a product's Halal. Pork is one type that is often used to mix with beef because the two types of meat have physical similarities if not carefully considered. DNA-based analysis, often used for halal authentication, is the real-time PCR method, so this study aims to prove that conventional PCR methods can detect DNA at the same concentration. This study uses two parameters: the specificity test carried out using pig DNA samples and cow and chicken DNA as a comparison. The second parameter is the detection limit test on absolute DNA carried out at 4 concentrations, namely 50, 5, 0.5 and 0.05 ng/µL, while the relative detection limit test (pork-cow mixture) with variations in pork concentration, namely 100%, 5%, 3%, 1%, and 0.5%. The analysis showed that the primers were specific to pig DNA with an absolute detection limit of 0.05 ng/µL and a relative detection limit of 0.5%. This PCR method meets the validation requirements for identifying target species so that it can be used for halal authentication of various products.
The Potency of Typical Plants of Central Sulawesi Province to Prevent Iron Deficiency Anemia: Experimental Study Mulia Sakti, Putri; Hi. Amir Sene, Ikhsan; Ramadhan, Kadar
Public Health of Indonesia Vol. 10 No. 1 (2024): January - March
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v10i1.775

Abstract

Background: Central Sulawesi falls within the Wallacea region, making it home to various endemic flora and fauna. One distinctive plant in Central Sulawesi is the Moringa tree. The Moringa tree, especially its leaves, was often used as a culinary ingredient known as sayurkelor in the traditional cuisine of the Kaili ethnic group in Central Sulawesi Province. Objective: This research aimed to determine the effectiveness of moringa leaf extract in increasing hemoglobin levels and erythrocyte count. Method: Twenty-four male Wistar rats strain (Rattusnorvegicus) 9−10 weeks old and 200−250 grams were divided into four groups (n=6), normal as a no treatment, negative control, ethanol extract, and positive control during the 15thday. Blood samples on the 15thday were administered to determine blood count. Result: The resultsof this study showed differences in hemoglobin and erythrocyte levels in the four experimental groups.The significant difference in average hemoglobin levels between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is significant with a P-value <0.05 (P value 0.00), and the difference between Group 3 and Group 4 is significant with a P-value <0.05 (P value 0.03). the significant difference in the average erythrocyte counts between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is not significant with a P-value >0.05 (P value 0.07). The difference between Group 3 and Group 4 has a P-value >0.05 (P value 0.63). Conclusion: The research results indicate that pregnant Wistar rats experienced hemodilution characterized by a decrease in hemoglobin and erythrocyte levels during pregnancy. However, after being given ethanol extract from moringa leaves, there was an increase in hemoglobin and erythrocyte levels. Keywords: Extract; ethanol; moringa; oleifera; hemoglobin
Gambaran Tingkat Pengetahuan Masyarakat Tentang Dagusibu di Desa Salubomba Kecamatan Banawa Tengah Kabupaten Donggala Ikhsan Hi. Amir Sene; Nurfadilah Nurfadilah
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 1 (2024): Januari : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i6.791

Abstract

Knowledge results from knowing and occurs when someone senses a particular object. Dagusibu (Get Use Save and Throw Away) is a health education program created by IAI (Indonesian Pharmacists Association) to create a Drug Aware Family Movement as a concrete step to improve the quality of life of the community. This research aims to determine the level of community knowledge about Dagusibu Medicine in Salubomba Village, Central Banawa District, Donggala Regency based on age, occupation, and education. The type of research is descriptive research. The population of the study is all people who live in Salubomba Village, Central Banawa District, Donggala Regency. The sample used was 94 people with each hamlet representing 19 people. The sampling technique uses purposive sampling. The results of the research showed that the level of public knowledge about Dagusibu Medicine in Salubomba Village was 39 respondents with poor knowledge, 51 respondents with sufficient knowledge, and 4 respondents with good knowledge. So, it can be concluded that the level of knowledge of the people in Salubomba Village has an adequate level of knowledge about Dagusibu Medicine. The conclusion that can be drawn is that the level of public knowledge about Dagusibu Medicine is sufficient knowledge, namely the level of public knowledge based on age level with less knowledge is 41.42%, based on occupation, less knowledge is 41.42%, and based on education, less knowledgeable is 41.48%.
Co-Authors Kadar Ramadhan Mariyani Ni nyoman Yuvita Yani Nurfadilah Nurfadilah Putri Mulia Sakti