Anis Herliyati Mahsunah
National Research and Inovation Agency

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Double step method in lipid extraction from biomass Aurantiochytrium sp powder Endah Sulistiawati; Suhendra Suhendra; Martomo Setyawan; Anis Herliyati Mahsunah
International Journal of Advances in Applied Sciences Vol 12, No 4: December 2023
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v12.i4.pp376-383

Abstract

Aurantiochytrium sp is a marine microalgae that is rich in lipids. Extraction of lipids from microalgae requires effort to select a suitable solvent and extraction method. This research used a double-step extraction method to study a mixture of n-hexane and methanol as a solvent. The variables studied were stirring time, the n-hexane to methanol (H/M) mixture ratio, and the solvent-to-biomass ratio (S/B). This research concluded that an optimum stirring time was 30 min, and a mixture of n-hexane and methanol solvents with a volume ratio of 1:1 is optimum. The optimum solvent-to-biomass ratio was S/B=20 mL/g dry microalgae. Under these conditions, the yield of oil was 83.88%. Double-step extraction can increase the yield by 10-40%.