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All Journal Jurnal Media Analis Kesehatan
Rafika Rafika
Prodi Sarjana Terapan Teknologi Laboratorium Medis, Poltekkes Kemenkes Makassar
Health Professions Immunology & microbiology Medicine & Pharmacology

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Potential of Cassava Peel as an Alternative Growth Media of Aspergillus niger and Rhizopus oryzae with Concentration Modification Artati Artati; Risky Risky; Rafika Rafika; Zulfian Armah; Syahida Djasang; Asriyani Ridwan; Aan Yulianingsih Anwar
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.251

Abstract

Waste is the waste product of a production process or materials that are no longer valuable or worthless, including waste from food processing processes. One of the main food wastes produced in developing countries is cassava peel from a plant with the Latin name Manihot ultisima. Cassava skin is the outer layer of the tuber which functions as a protector for the cassava flesh. This research aims to prove that cassava peel can be used as raw material for making alternative media for fungal growth and to find out the most effective concentration to use. This research is experimental using cassava skin as a sample. In this research, data analysis was carried out using the One-Way Anova Test. Concentrations of 5%, 10%, 15%, 20%, 25% and 30% were made as alternative media for the growth of Aspergillus niger and Rhizopus oryzae fungi and SDA media as a control. During the incubation period for these two species, both Aspergillus niger and Rhizopus oryzae experienced growth which became bigger day by day. The conclusion of this research is that cassava peel can be used as a natural ingredient that can be used as an alternative medium for fungal growth. In the alternative media for cassava peel, the most effective concentration of the Aspergillus niger species is 20%, while for the Rhizopus oryzae species it is 15%
Potencial of Rice Bran Extract as a Basic Ingridient in Candida albicans Culture Media Rafika Rafika; Rahman Rahman; Ridho Pratama; Putri Sri Saqinah Sudirman; Mursalim Mursalim
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.275

Abstract

Rice bran is a natural material that contains nutrients that serves as a fungal growth factor, can be used as an alternative media base for fungal growth. The aim of this research is to determine the potential of rice bran as an alternative medium for the growth of Candida albicans. The research design employed an experiment that compared the growth of Candida albicans on rice bran extract media and Sabouraud Dextrose Agar (SDA) media. The sample is rice bran powder obtained from rice mills and rice bran extract was made using a multi-stage pretreatment method. The rice bran extract was used to create Rice bran Agar (RBA) and Rice bran Liquid (RBC) media, while SDA dan (SDB) media were also prepared. The media were inoculated with Candida albicans, incubated, and observed for 5 days. Data analysis included the measurement of fungal colony diameter using One Way Anova and Candida albicans biomass using an independent t-test. The research findings indicate that the average diameter of fungal colonies on RBA extract media was 10.4 mm, whereas it was 12.3 mm on SDA media. The Anova and Post Hoc Tukey HSD results demonstrated significant daily differences in colony diameter on RBA media, indicating notable Candida albicans growth. Conversely, there was no significant difference in colony diameter on SDA media. The biomass average of Candida albicans on RBC media was 0.82 g, compared to 0.45 g on SDB media. The Mann-Whitney test (p < 0.05) indicated a significant contrast in the average dry weight of Candida albicans biomass, with RBC media being heavier than SDB media. The conclusion is RBA and RBC media have the ability to grow Candida albicans which can be used as an alternative to SDA and SDB media.
Co-Authors Aan Yulianingsih Anwar Artati Artati Mursalim Mursalim Putri Sri Saqinah Sudirman Rahman Rahman Ridho Pratama Risky Risky Syahida Djasang Zulfian Armah