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Prodi Sarjana Terapan Teknologi Laboratorium Medis, Poltekkes Kemenkes Makassar

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Potential of Cassava Peel as an Alternative Growth Media of Aspergillus niger and Rhizopus oryzae with Concentration Modification Artati Artati; Risky Risky; Rafika Rafika; Zulfian Armah; Syahida Djasang; Asriyani Ridwan; Aan Yulianingsih Anwar
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.251

Abstract

Waste is the waste product of a production process or materials that are no longer valuable or worthless, including waste from food processing processes. One of the main food wastes produced in developing countries is cassava peel from a plant with the Latin name Manihot ultisima. Cassava skin is the outer layer of the tuber which functions as a protector for the cassava flesh. This research aims to prove that cassava peel can be used as raw material for making alternative media for fungal growth and to find out the most effective concentration to use. This research is experimental using cassava skin as a sample. In this research, data analysis was carried out using the One-Way Anova Test. Concentrations of 5%, 10%, 15%, 20%, 25% and 30% were made as alternative media for the growth of Aspergillus niger and Rhizopus oryzae fungi and SDA media as a control. During the incubation period for these two species, both Aspergillus niger and Rhizopus oryzae experienced growth which became bigger day by day. The conclusion of this research is that cassava peel can be used as a natural ingredient that can be used as an alternative medium for fungal growth. In the alternative media for cassava peel, the most effective concentration of the Aspergillus niger species is 20%, while for the Rhizopus oryzae species it is 15%
Modification of the Calculation of the Examination of the Platelet Count Using 1 Large Box of Leukocytes in the Improved Neubauer Counting Room Zulfikar Ali Hasan; Nurdin Nurdin; Ridho Pratama; Artati Artati; Yaumil Fachni Tandjungbulu; Andi Fajrin Permana
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.256

Abstract

The direct examination of the platelet count using the improved Neubauer counting chamber is carried out by microscopic examination of 25 Medium Boxes of Erythrocytes, this method requires several field shifts so that more accuracy is needed when carrying out the examination. Modification of the calculation of the platelet count by using 1 large box of leukocytes in the improved Neubauer counting chamber requires only 1 field of view at the time of calculation so that it does not take a long time and is more thorough when carrying out microscopic platelet counts. The purpose of this study was to determine the number of platelets using the calculation of 25 squares while the erythrocytes and the number of platelets using a modification of the calculation of 1 large box of leukocytes. This type of research is a quasi-experimental which aims to determine the effect that arises due to certain treatments for examining the platelet count using 25 boxes while the erythrocytes and modification of the calculation using 1 large box of leukocytes. It was carried out at the Hematology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Makassar. This research was carried out to start in January-December 2022. Examination of the number of platelets in the counting chamber with 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes, from the 30 data obtained for each method after a different test using the Mann Whitney test obtained a significant p value of 0.234 > 0.05 so that it can be concluded that there is no difference in the number of platelets in 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes. This indicates that examination of the platelet count using 1 large box of leukocytes can be used as a manual examination method