Ridho Pratama
Prodi Sarjana Terapan Teknologi Laboratorium Medis, Poltekkes Kemenkes Makassar

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Modification of the Calculation of the Examination of the Platelet Count Using 1 Large Box of Leukocytes in the Improved Neubauer Counting Room Zulfikar Ali Hasan; Nurdin Nurdin; Ridho Pratama; Artati Artati; Yaumil Fachni Tandjungbulu; Andi Fajrin Permana
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.256

Abstract

The direct examination of the platelet count using the improved Neubauer counting chamber is carried out by microscopic examination of 25 Medium Boxes of Erythrocytes, this method requires several field shifts so that more accuracy is needed when carrying out the examination. Modification of the calculation of the platelet count by using 1 large box of leukocytes in the improved Neubauer counting chamber requires only 1 field of view at the time of calculation so that it does not take a long time and is more thorough when carrying out microscopic platelet counts. The purpose of this study was to determine the number of platelets using the calculation of 25 squares while the erythrocytes and the number of platelets using a modification of the calculation of 1 large box of leukocytes. This type of research is a quasi-experimental which aims to determine the effect that arises due to certain treatments for examining the platelet count using 25 boxes while the erythrocytes and modification of the calculation using 1 large box of leukocytes. It was carried out at the Hematology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Makassar. This research was carried out to start in January-December 2022. Examination of the number of platelets in the counting chamber with 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes, from the 30 data obtained for each method after a different test using the Mann Whitney test obtained a significant p value of 0.234 > 0.05 so that it can be concluded that there is no difference in the number of platelets in 25 Medium Boxes of Erythrocytes and 1 Large Box of Leukocytes. This indicates that examination of the platelet count using 1 large box of leukocytes can be used as a manual examination method
Potencial of Rice Bran Extract as a Basic Ingridient in Candida albicans Culture Media Rafika Rafika; Rahman Rahman; Ridho Pratama; Putri Sri Saqinah Sudirman; Mursalim Mursalim
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.275

Abstract

Rice bran is a natural material that contains nutrients that serves as a fungal growth factor, can be used as an alternative media base for fungal growth. The aim of this research is to determine the potential of rice bran as an alternative medium for the growth of Candida albicans. The research design employed an experiment that compared the growth of Candida albicans on rice bran extract media and Sabouraud Dextrose Agar (SDA) media. The sample is rice bran powder obtained from rice mills and rice bran extract was made using a multi-stage pretreatment method. The rice bran extract was used to create Rice bran Agar (RBA) and Rice bran Liquid (RBC) media, while SDA dan (SDB) media were also prepared. The media were inoculated with Candida albicans, incubated, and observed for 5 days. Data analysis included the measurement of fungal colony diameter using One Way Anova and Candida albicans biomass using an independent t-test. The research findings indicate that the average diameter of fungal colonies on RBA extract media was 10.4 mm, whereas it was 12.3 mm on SDA media. The Anova and Post Hoc Tukey HSD results demonstrated significant daily differences in colony diameter on RBA media, indicating notable Candida albicans growth. Conversely, there was no significant difference in colony diameter on SDA media. The biomass average of Candida albicans on RBC media was 0.82 g, compared to 0.45 g on SDB media. The Mann-Whitney test (p < 0.05) indicated a significant contrast in the average dry weight of Candida albicans biomass, with RBC media being heavier than SDB media. The conclusion is RBA and RBC media have the ability to grow Candida albicans which can be used as an alternative to SDA and SDB media.