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Wijaya, Hendra
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Mutagenicity Study of Pandanus Conoideus Oil Maeda, Takanori; Miyakita, Haruka; Goto, Manami; Ito, Akemi; Wijaya, Hendra; S. Surono, Inggrid; Nishigaki, Toshiaki
Warta Industri Hasil Pertanian Vol 30, No 02 (2013)
Publisher : Balai Besar Industri Agro

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Abstract

Pandanus conoideus (buah merah or tawi) is exclusively grown in Papua island and its neighbor areas, and indigenous people have been consuming it as functional food for thousand years. We have reported safety and anti-tumor effects of Buah Merah extract oil (S.B.M) in experimental animals. However, mutagenicity or genotoxicity of S.B.M has not been evaluated. We carried out mutagenetic evaluation of S.B.M by in vitro Ames test using salmonella typhimurium  TA98, TA100, TA1535 and TA1537 and escherichia coli WR2uvrA with or withou the activation of S9 mixture. Concentrations used for this test were 313-500ug/plate. The results show that there is no increase in revertant colonies, suggesting that S.B.M has no mutagenic activities under the conditions of this study.
(The Effectiveness of Bamboo Charcoal as a Cigarette Smoke Filter) Junaidi, Lukman; Wijaya, Hendra
Warta Industri Hasil Pertanian Vol 28, No 01 (2011)
Publisher : Balai Besar Industri Agro

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Abstract

Research on effectiveness effectiveness of using bamboo charcoal for cigarette smoke filter has been conducted .the aim of research was to investigate the characteristics of bamboo charcoal as a cigarette smoke filter .the results show the type of bambo charcoal andong and betung as well as particle size give no significance influence on benzene and chloroform adsorption ,while in contrarily give significance influence on significance influence on benzene  and chloroform adsorption,while in contrarily give significance influence on 0idium adsorption .usingthe cigarette filter added with bamboo charcoal andong and betung could increase 90% tar adsorption in cigarette smoke compare to tar adsorption by cigarette filter without bamboo charcoal added .while bamboo charoal filter andong and betung could increase nicotine adsorption in cigarette smoke only 45% and 19% respectively compare to tar adsorption by cigarette filter without bamboo charcoal added. The size of mesh of bamboo charcoal filter andong and betung give same level of influence on tar and nicotine adsorption.the lower the size of mesh the higher the effectiveness on tar and nicotine adsorption.
Identifikasi Kandungan Skopoletin dalam Berbagai Jenis Umbi-Umbian Wijaya, Hendra; Ramadhan Ningrum Has, Dinia; Febriyanti, Erna; Anwar, Chaerul
Warta Industri Hasil Pertanian Vol 31, No 01 (2014)
Publisher : Balai Besar Industri Agro

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Abstract

Skopoletin merupakan senyawa kumarin yang telah dibuktikan secara ilmiah mempunyai efek kesehatan terhadap manusia.  Efek kesehatan skopoletin diantaranya adalah sebagai antikanker, antioksidan, antidiabetes tipe 2, antihipertensi, antibakteri, memperbaiki memori atau daya ingat, dan pengendalian stres serta bad mood.  Berdasarkan hal tersebut, penting untuk mengetahui sumber pangan yang mengandung skopoletin.  Penelitian ini bertujuan untuk menentukan kandungan skopoletin dalam berbagai jenis umbi-umbian yang ada di Indonesia.  Metode analisis skopoletin yang digunakan adalah metode kromatografi cair kinerja tinggi-fluoresensi (KCKT-FL).  Hasil analisis menunjukkan bahwa skopoletin terdapat pada kulit dan daging ubi kayu, ubi jalar, dan ganyong.  Skopoletin tidak terdapat pada talas, garut, dan iles-iles.  Kandungan skopoletin tertinggi terdapat pada daging ubi kayu jenis Malang 4 yaitu sebesar 112.66 mg/kg (berat kering) sedangkan pada ubi jalar terdapat pada kulit ubi jalar Cilembu sebesar 69.73 mg/kg (berat kering).
(The Study of Making Bamboo Charcoal and Vinegar using Pyrolisis Kiln of Semi Commercial Scale) Pohan, H.G; Wijaya, Hendra; Suherman, Ade
Warta Industri Hasil Pertanian Vol 27, No 01 (2010)
Publisher : Balai Besar Industri Agro

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Abstract

Study on production of bamboo charcoal an vinegar have been carried out semi commercially using pyrolysis furnace with a volume of 6 m3. The result of this study is expected to be a model of processing bamboo charcoal and vinegar for rural area. Observations were made on the characteristics of pyrolysis, yield of charcoal and bamboo vinegar, and characteristics of charcoal i.e., water content, ash, volatile matter, fixed carbon, calories, and pH. The bamboo raw material availability and techno-economical analysis were also conducted. The study revealed that the temperature of the out coming gar during the pyrolization process was 300 to 350 oC, the average yield of charcoal and vinegar were 27.33% and 33.61% respectively. The resulted bamboo charcoal has water content of 3.06%, volatile matter at 950oC was 40.03% (wb), ash content was 7.2% (wb), fixed carbon of 52.37% (wb) and calorific value of 5777 cal/g. The resulted bamboo vinegar has pH 4 and its refractive index was ranged from 3.25 - 3.75. The availability of raw material bamboo in the Village at Desa Tenjojaya, Kecamatan Cibadak Kabupaten Sukabumi, within the radius of 4 km was 3620 ton, while the possible selling price of the resulted charcoal was Rp. 3.000.-/kg whereas the vinegar was Rp.5000.-/kg. The techno-economical analysis of the processing unit of bamboo charcoal and vinegar with the capacity of 10 tons of charcoal per month revealed Present Value (NPV) of Rp.596.000.000,-, the Internal Rate pf Return (IRR) of 78% or Pay-back period was 1.3 years.
(Studi Toksisitas Akut dan Subaku dari Ekstrak Minyak Buah Merah pada Tikus Sprague Dawley) Nishigaki, Toshiaki; N.A. Dewi, Fitriyai; Wijaya, Hendra; Shigematsu, Hidekazu
Warta Industri Hasil Pertanian Vol 28, No 01 (2011)
Publisher : Balai Besar Industri Agro

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Abstract

Pandanus conoideus is exclusively grown in papua island and its neighbor areas and its fruit (buah merah) has been utilized as food supplement by the native papua people for a long period. recently it was found that its extract oil (SMB) contains contents of carotenoids ,especially a novel micronutrient beta-cryptoxanthin .we consider that SBM is a potent chemopreventive supplement against some types of cancer and provide health benefits for chronic diseases .in the present studies, we evaluated acute ad subacute toxicities of SBM using Sprague Dawley rats.Oral acute toxicity of SBM using female rats was not observed and LD 50 was more than 2 ml/kg in subacute toxicity study ,SMB was orally administered at 0.1,0.3 and 1.0 ml/kg to male and female rats for consecutive 4 weeks .no findings associated with SBM observed we consecutive 4 weeks .no findings associated with were observed we conclude that SBM is a safe food supplement.
(Manihot utilissima Pohl.)(The Effects of Sucrose and Citric Acid Concentration on the Co-crystallization of Fermented Cassava (Manihot utilissina Pohl) Extract) Neffi Ridwan, Indra; Sriyogasari, Titin; Wijaya, Hendra; Turmala Sutrisno, Ela
Warta Industri Hasil Pertanian Vol 21, No 02 (2004)
Publisher : Balai Besar Industri Agro

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Abstract

The purpose of this research is to study the effect of interaction between sucrose and citric acid concentration on the quality of co-crystallization of fermented cassava (manihot utillisima pohl). Extract. The benefit of this research is to diversify cassava fermented product in order to type of food product that is more pratical in its consumption. s a result this product will create an increasing economic value of cassava. The experiment was carried out using the randomized block desing withpattern factorial and 3 times replicating. The frist factor is sucrose concentration (40%; 50%; and 60%) and the second factor is citric acid concentration (1% ; 1.5%; and 2%). Parameter analyzed during the experiment were moinsture content, total sugar, solubility, and organoleptic test using prefence test type with 15 panelist which cover color, taste, flavor, and apperance. Basedc on the result of organoleptic test of microcrystal fermented cassava extract that perferred by panelist were it was produced with 40% of surcose and 1.5% of citric acid concentraction.  This fermented cassava extract produced by this methods contain moinsture content 2.14% and total sugar 56.05%, and has solubility 26.84%.
(Method for HPLC Analysis of Active Compounds in Ginger (Zingiber officinale) Maria Novelina.S, Yus; Maman Rohaman, M; Wijaya, Hendra
Warta Industri Hasil Pertanian Vol 28, No 02 (2011)
Publisher : Balai Besar Industri Agro

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Abstract

The studies Ginger (zingiber officinale) is one of herb plant which contains various of active compounds,which is proved has function has  to human health .in indonesia , there are 3 main ginger variaties,those are :giant gingger (zingiber officinale var .officinale),emprit ginger (Zingiber officinale var. amarum ), and red ginger (Zingiber officinale var.rubrum) .Each ofthem has different characteristic and function .the problems which often occurs are ginger counterfeting,often mixed with other gingers.this counterfeiting difficult to be detected with conventional test method. therefore needed study about characteristic of each ginger with HPLC so counterfeiting able to be traced .Asensitive and accurate high-performance liquid chromatography (HPLC) method has been developed to determine the quantity of active componen in the methanolic extract of Zingiber officinale Bioactive component was separated on persuit C18 Column (250 mm x4.6 mm,particle size:5 um)with gradient system of water and acetonitrile as mobile phase. the calibration plot was linear in the range of 0.10-150 mg/L .of 6-,8-,10-gingerol and the correlation coefficient of o.9998 was indicative of good linear dependence of peak area on concentration .the limit of detection (LOD) OF 6-gingerol ,8-ginggerol ,6-shogaol and  6-shogaol and the correlation coefficient were 2,34 1,06; 1,25, and 0,67 mg/l and limit of detection (LOD)were 7,81 :3,53 :3,53; 4,18 and 2,29 mg/lrespectively.the developed HPLC method was found selective,precise and accurate,and can be used for routine analysis of gingers in the quality control laboratories .
(Antioxidants : Mechanism of Action and Its Function in Human Body) Wijaya, Hendra; Junaidi, Lukman
Warta Industri Hasil Pertanian Vol 28, No 02 (2011)
Publisher : Balai Besar Industri Agro

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Abstract

Antioxidant could be defined by several means. Antioxidant is a substance that could postpone and delay oxidation of molecule substrate in human body ,i.e ; protein ,carboydtrate,lipid and DNA, by oxidant. antioxidant that works in the human bodys biological system is called a  biological antioxidant Biological antioxidants can be grouped into :1) enzymatic antioxidants (endogenous) ,2) non-enzymatic antioxidants (endogenaous) and, 3) antioxidants from food (exogenous0.Enzymatic antioxidants (endogenous) include superoxide dismutase ,catalase ,glutathione peroxidase, glutaredoksin, tioredoksin, peroksiredoksin ,and coenzyme q10.non-enzymatic antioxidants (ENDOGENOUS) are carotenoids,vitamin E  and vitamin C those antioxidants are able to work in hydrophobic and hydrophilic cellular environment so as to from a strong network of cooperation in preventing the occurrence of oxidative damage .some of the functions of antioxidants in the human body that can be expressed are ; preventive defense the human body against oxidants ,strong defense system against free redicals ,prevent the occurrence atherosclerosis in blood vessels,regulation of metabolism such as disabling lipoksigenase.
Co-Authors Anwar, Chaerul Dewi, Fitriya Nur Annisa Febriyanti, Erna Goto, Manami Ito, Akemi Junaidi, Lukman Maeda, Takanori Maman Rohaman, M Maria Novelina.S, Yus Miyakita, Haruka Neffi Ridwan, Indra Nishigaki, Toshiaki Pohan, H.G Ramadhan Ningrum Has, Dinia S. Surono, Inggrid Shigematsu, Hidekazu Sriyogasari, Titin Suherman, Ade Toshiaki NISHIGAKI Turmala Sutrisno, Ela