Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST RESLAJ: Religion Education Social Laa Roiba Journal
Endin Nokik Stujanna
Unknown Affiliation
Religion Economics, Econometrics & Finance Education Environmental Science Health Professions Social Sciences

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Specific Primer Design to Detect Connexin-36 Gene in Rattus Norvegicus Brain Sri Suciati Ningsih; Alfina Septiasari; Endin Nokik Stujanna; Rizkyana Avissa; Irena Ujianti; Shinta Dewi Permatasari; Wawang S. Sukarya
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.17985

Abstract

Connexin-36 (Cx36) merupakan salah satu protein kanal antar sel. Protein ini paling banyak ditemukan pada otak. Gen Cx36 mengekspresikan protein penghubung Cx36 yang membentuk sinaps listrik. Penelitian ini bertujuan untuk memperoleh desain primer terbaik untuk menganalisis ekspresi Cx36 pada otak tikus dengan metode Quantitative Real Time PCR (qRT-PCR). Jenis penelitian ini adalah penelitian true experimental menggunakan mRNA dari 4 otak tikus. Analisis in silico menggunakan tools Primer 3 dan OlygoAnalizerTM by IDTdengan menggunakan database gen pada NCBI. Tiga primer dengan kriteria yang paling optimum kemudian dievaluasi dengan metode qRT-PCR. Berdasarkan hasil penelitian ketiganya mampu mengamplifikasi gen Cx36 dengan menggunakan qRT-PCR. Hasil primer terbaik menunjukkan melt curve tunggal pada primer no.5 gen Cx36 pada kisaran Tm 80-81oC. Hasil perbandingan uji in silico primer Cx36 yang sudah pernah dipublikasi sebelumnya menunjukkan bahwa primer no. 5 juga memiliki nilai optimal pada setiap kriteria. Maka hasil desain primer yang memiliki spesifisitas dan efektifitas yang optimum untuk mengamplifikasi gen Cx36 pada otak tikus Rattus norvegicus ialah primer no.5 (F: 5’-ATTTCCCGCTTCTACATCATCCAAG-3’ dan R: 5’-CACAGCAAACATGAACACCAGAAAG-3’).
Hubungan Perilaku Cerdik Terhadap Hipertensi di Puskesmas Kelurahan Pekayon Tahun 2024 Mochammad Farid Cahya Saputra; Zainal Abidin; Rizka Aries Putranti; Endin Nokik Stujanna; Enrico Adhitya Rinaldi
Reslaj: Religion Education Social Laa Roiba Journal Vol. 6 No. 9 (2024): RESLAJ: Religion Education Social Laa Roiba Journal 
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/reslaj.v6i9.4912

Abstract

Hypertension is a non-communicable disease that increases every year. The CERDIK program is one of the non-pharmacological treatments that can be used in an effort to prevent hypertension. This study aims to determine the relationship between smart behavior and hypertension at the Pekayon Village Health Center in 2024. This study uses a quantitative method with a Cross Sectional design. The sample consisted of 96 respondents using Accidental Sampling as the sampling technique. Data analysis used univariate, bivariate, and multivariate analysis. This study shows that at the Pekayon Village Health Center there are 91 people (71.7%) who routinely do health checks. Avoiding cigarette smoke is in the good category as many as 88 people (69.3%), diligently doing physical activity as many as 84% ​​(66.1%), having a balanced diet as many as 88 people (69.3%), getting enough rest as many as 84 people (66.1%), and being able to manage stress well as many as 105 people (82.7%). The results of this study indicate that there is a relationship between CERDIK behavior and hypertension with a p value of <0.05, so there is a correlation between CERDIK behavior and the level of hypertension in patients at the Pekayon Village Health Center.
Co-Authors Alfina Septiasari Enrico Adhitya Rinaldi Irena Ujianti Mochammad Farid Cahya Saputra Rizka Aries Putranti Rizkyana Avissa Shinta Dewi Permata Sari Sri Suciati Ningsih Wawang S. Sukarya Zainal Abidin