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All Journal Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Sains: Jurnal Kimia & Pendidikan Kimia Jurnal Teknologi Pangan dan Ilmu Pertanian
Wa Ode Mulyana
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Agriculture, Biological Sciences & Forestry Education

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Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Belimbing Wuluh (Aveerrhoa bilimbi L.) dengan Metode DPPH Ika Fatmawati S; Haeruddin; Wa Ode Mulyana
Sains: Jurnal Kimia dan Pendidikan Kimia Vol. 12 No. 1 (2023): EDISI JUNI 2023
Publisher : Jurusan Pendidikan Kimia FKIP Universitas Halu Oleo, Jl. HEA. Mokodompit Kampus Hijau Bumi Tridharma Andounohu Kendari, Gedung MIPA FKIP UHO Sulawesi Tenggara – Indonesia

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Abstract

A study "Test Antioxidant Activity of Ethyl Acetate Extract of startfruit Leaves (Averrhoa bilimbi L.) with the DPPH Method" with the aim is to determine the types of identified secondary metabolites identified in the ethyl acetate extract of starfruit leaves, and to determine the antioxidant activity of the ethyl acetate extract of starfruit leaves wuluh. Wuluh starfruit leaves used as much as 250 grams which were macerated with technical ethyl acetate solvent and obtained a thick ethyl acetate extract of 12.62%. The group of secondary metabolites was determined by the phytochemical method, and the method used to determine the antioxidant activity was the DPPH method. The ethyl acetate extract of belimbing wuluh leaves contains alkaloids, steroids, tannins and has a very low antioxidant activity with an IC50 value of 547.56 ± 0.0196 ppm, while vitamin C has a very strong antioxidant activity with an IC50 value of 2.33 ± 0. 1786. Based on this IC50 value, vitamin C is the strongest antioxidant from the ethyl acetate extract of belimbing wuluh leaves, and this leaf extract is classified as a very weak antioxidant.
Deskripsi Kualitas Minyak Goreng Hasil Pemanasan Zuliyama; Rahmanpiu; Wa Ode Mulyana
Sains: Jurnal Kimia dan Pendidikan Kimia Vol. 12 No. 1 (2023): EDISI JUNI 2023
Publisher : Jurusan Pendidikan Kimia FKIP Universitas Halu Oleo, Jl. HEA. Mokodompit Kampus Hijau Bumi Tridharma Andounohu Kendari, Gedung MIPA FKIP UHO Sulawesi Tenggara – Indonesia

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A research entitled description of cooking oil quality resulted from heating. This study aims to describe the quality of cooking oil produced by heating. This research is a quantitative descriptive which tests on 3 variables, namely water content, free fatty acid content and peroxide value of cooking oil before and after heating. Water content was determined by thermogravimetry method, free fatty acid content was determined by alkalimetric titration method (KOH) and the peroxide number was determined by iodometric method. The results showed that in determining the water content, the three types of oil met the SNI 7709 (2019) standard with the value of packaged oil I, packaged oil II and bulk oil respectively 0.1197%, 0,0749% and 0,1048%. In determining the free fatty acid content without heating, none met the SNI 7709:2019 standard, while in the determination of the free fatty acid content after heating, the three types of oil met the SNI 7709 (2019) standard with successive values of 0.334%, 0.2319% and 0.3141%. In determining the peroxide value without heating, the three types of oil met the SNI 7709 (2019) standards with successive values of 6.7217 mek O2/kg, 8.7664 mek O2/kg and 9.6556 mek O2/kg, while in determining the peroxide number after heating, only oil packaged II cooking oil and bulk cooking oil that meet the SNI 7709 (2019) standards with values of 7.8949 mek O2/kg and 9.6556 mek O2/kg respectively.
Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Fraksi N-Heksan dan Fraksi Etil Asetat Ekstrak Etanol Daun Ketepeng Cina (Cassia alata L.) Nunung Pratiwi; Dahlan; Wa Ode Mulyana
Sains: Jurnal Kimia dan Pendidikan Kimia Vol. 12 No. 2 (2023): EDISI DESEMBER 2023
Publisher : Jurusan Pendidikan Kimia FKIP Universitas Halu Oleo, Jl. HEA. Mokodompit Kampus Hijau Bumi Tridharma Andounohu Kendari, Gedung MIPA FKIP UHO Sulawesi Tenggara – Indonesia

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This study aims to determine the group of secondary metabolite contained in n-hexane fraction and ethyl acetate fraction ethanol extracts chinese ketepeng leaves and determine the level of toxicity n-hexane fraction and ethyl acetate fraction extracts chinese ketepeng leaves on Artemia salina L. Extraction using maceration technique, the secondary metabolite compounds was determined by phytochemical method, and toxicity testing on Artemia salina L. larvae by BSLT method. Larval mortality data were analyzed by probit analysis to determine the LC50 value in each n-hexane fraction and ethyl acetate fraction. The results of phytochemical screening showed the presence the n-hexan fraction contains flavonoids and steroid compounds, while the ethyl acetate fraction contains steroid and tannin compounds. The results of toxicity testing showed that each fraction showed toxic activity against Artemia salina Leach larvae. The ethyl acetate fraction showed the highest toxic activity (LC50 of 316,22 ppm), and the n-hexan fraction (LC50 of 457,08 ppm).
Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Fraksi n-Heksan dan Fraksi Etil Asetat Ekstrak Etanol Batang Ketepeng Cina (Cassia alata L.) Sri Sartini Muniaha; Dahlan; Wa Ode Mulyana
Sains: Jurnal Kimia dan Pendidikan Kimia Vol. 13 No. 1 (2024): EDISI JUNI 2024
Publisher : Jurusan Pendidikan Kimia FKIP Universitas Halu Oleo, Jl. HEA. Mokodompit Kampus Hijau Bumi Tridharma Andounohu Kendari, Gedung MIPA FKIP UHO Sulawesi Tenggara – Indonesia

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Penelitian ini bertujuan untuk mengidenfikasi kandungan metabolit sekunder yang terkandung dalam fraksi n-heksan dan dan fraksi etil asetate dari ekstrak etanol batang ketepeng cina, juga menganalisis aktifitas toksiknyadalam menghambat larva A. salina. Setiap fraksi dianalisis secara fotokimia dan diuji toksisitasnya dengan metode Brine Shrimp Lethality Test (BSLT). Hasil pengujian menunjukkan bahwa fraksi n-heksan mengandung steroid dan saponin, sementara fraksi etil asetate positif flavonoid dan tanin. Kedua fraksi memperlihatkan aktivitas penghambatan terhadap larva A. salina Leach. Fraksi n-heksan memperlihatkan aktifitas yang sangat tinggi (LC50 of 193,95 ppm), sedikit lebih rendah dari fraksi etil asetat (LC50 of 274,026 ppm).
Pelatihan Pembuatan Cocopeat dari Sabut Kelapa Muda untuk Bahan Baku Media Tanam Rahmanpiu, Rahmanpiu; Murni Nia; Samsul Alam Fyka; La Malesi; Wa Ode Mulyana
Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2023): Edisi November 2023
Publisher : FKIP Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36709/amalilmiah.v5i1.97

Abstract

Pemerintah Republik Indonesia melalui Kementerian Lingungan Hidup dan Kehutanan mencanangkan “Tuntas Kelola Sampah untuk Kesejahteraan Masyarakat”. Sabut kelapa muda bila diolah dapat dimanfaatkan sebagai pupuk organik untuk media tanam. Sabut kelapa muda hingga saat ini belum dimanfaatkan. Karena itu, Tim PKM melakukan pelatihan pembuatan cocopeat dari sabut kelapa muda. Tujuan kegiatan ini adalah memberikan pelatihan dan pendamping kepada mitra untuk ; (1) membuat alat penghancur sabut kelapa dari rantai bekas mesin pemotong kayu,  (2) melakukan fermentasi serbut sabut kelapa muda, dan (3) melakukan uji coba media tanam pada budidaya tanaman kangkong dan sawi. Metode pelaksanaan  menggunakan metode penyuluhan partisipasif dimana mitra terlibat secara langsung dalam kegiatan pengabdian. Hasil yang dicapai adalah mitra dapat (1) mengembangkan Teknologi Tepat Guna mesin slinder penghancur sabut kelapa muda dari rantai bekas mesin pemotong kayu dengan kinerja ± 70 kg/jam. (2) menghasilkan cocopeat yang memiliki tekstur yang sama dengan cocopeat dari sabut kelapa tua. (3) formulasi media tanam untuk uji coba budidaya kangkong dan sawi adalah tanah, cocopeat, kompos dan abu sekam (2:1:1:0,5). Hasil budidaya menunjukkan bahwa budidaya tanaman kangkung dan sawi memberikan hasil baik, berwarna kehijauan, tidak tampak hama, dan panjang kangkung berkisar antara 20 – 40 cm, sedangkan sawi dengan Panjang 15 – 30 cm.
Profil Fitokimia dan Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Mete (Anacardium Occidentale Linn) Baihaqi Baihaqi; Fatahu, Fatahu; Andi Laila Nugrawati; Wa ode Mulyana; Eka Cahyana Mandasari
Jurnal Teknologi Pangan dan Ilmu Pertanian Vol. 2 No. 4 (2024): Desember: Jurnal Teknologi Pangan dan Ilmu Pertanian
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59581/jtpip-widyakarya.v3i1.4699

Abstract

Cashew leaves or also called monkey guava are known by many people, especially in Southeast Sulawesi and are believed to have a number of health benefits but are still rarely known by some people. This study aims to determine the profile of secondary metabolite content and determine the antioxidant activity of ethanol extract of cashew leaves (Anacardium occidentale Linn) using the extraction method by maceration. The extraction process of old cashew leaves is carried out by the maceration method using 96% ethanol solvent for 3 days of soaking and stirring every 24 hours, then concentration is carried out through the evaporation process. Phytochemical testing includes identification of alkaloids, flavonoids, polyphenols, tannins, steroids/triterpenoids and saponins, as well as testing antioxidant activity using the DPPH method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the extract of cashew leaves (Anacardium occidentale Linn) strongly positive contains phenolic compounds, flavonoids, steroids and saponins. Based on the results of the IC50 calculation, 96% ethanol extract has the highest antioxidant activity at a concentration of 14.35 ppm (µg/mL) with a very high category. The content of secondary metabolites and very high antioxidant activity from the results obtained, explain that cashew nuts have the potential to increase body immunity and can be used in various antioxidant food products so that cashew leaves are more economically valuable for the community.
Co-Authors Baihaqi Baihaqi Dahlan Dahlan Eka Cahyana Mandasari Fatahu, Fatahu Haeruddin Ika Fatmawati S La Malesi Murni Nia Nunung Pratiwi Rahmanpiu Rahmanpiu, Rahmanpiu Samsul Alam Fyka Sri Sartini Muniaha Zuliyama