Ratu Choesrina
Prodi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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UJI AKTIVITAS ANTIJAMUR EKSTRAK ETANOL KULIT BUAH PETAI (PARKIA SPECIOSA HASSK.) TERHADAP PERTUMBUHAN CANDIDA ALBICANS DAN MICROSPORUM GYPSEUM Luzhny Azzahra; Lanny Mulqie; Ratu Choesrina
Bandung Conference Series: Pharmacy Vol. 3 No. 2 (2023): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v3i2.9118

Abstract

Abstract. Petai plant (Parkia speciosa Hassk.) is known to contain metabolite compounds that can be developed to treat several diseases, one of which is antifungal. This study aims to determine the antifungal activity of ethanol extract of petai fruit peel (Parkia speciosa Hassk.) against the growth of Candida albicans and Microsporum gypseum, and determine the value of the inhibition zone in ethanol extract of petai fruit peel (Parkia speciosa Hassk.) against the growth of Candida albicans and Microsporum gypseum. The method used was agar diffusion (wells). The concentrations of the extracts tested were 1, 5, 10, 15, and 20%. The positive control used was ketoconazole 2% and the negative control was DMSO. The results of the calculation of the average zone of inhibition of ethanol extract of petai fruit peel against Candida albicans at concentrations of 1, 5, 10, 15, and 20% were 13,9; 15,5; 17,6; 18,9; and 20,3 mm, respectively. The results of the calculation of the inhibition zone of ethanol extract of petai fruit peel against Microsporum gypseum at concentrations of 1, 5, 10, 15, and 20% were 17,5; 19,8; 18,6; 23,7; and 26,6 mm, respectively. The results showed that ethanol extract of petai fruit peel (Parkia speciosa Hassk.) has antifungal activity with the formation of an inhibition zone around the wells. Abstrak. Tanaman petai (Parkia speciosa Hassk.) diketahui memiliki kandungan senyawa metabolit yang dapat dikembangkan untuk mengobati beberapa penyakit, salah satunya antijamur. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak etanol kulit buah petai (Parkia speciosa Hassk.) terhadap pertumbuhan Candida albicans dan Microsporum gypseum, serta mengetahui nilai zona hambat pada ekstrak etanol kulit buah petai (Parkia speciosa Hassk.) terhadap pertumbuhan Candida albicans dan Microsporum gypseum. Metode yang digunakan adalah difusi agar (sumuran). Konsentrasi ekstrak yang diuji yaitu 1, 5, 10, 15, dan 20%. Kontrol positif yang digunakan yaitu ketokonazol 2% dan kontrol negatif yaitu DMSO. Hasil perhitungan rata-rata zona hambat ekstrak etanol kulit buah petai terhadap Candida albicans pada konsentrasi 1, 5, 10, 15, dan 20% berturut-turut sebesar 13,9; 15,5; 17,6; 18,9; dan 20,3 mm. Hasil perhitungan zona hambat ekstrak etanol kulit buah petai terhadap Microsporum gypseum pada konsentrasi 1, 5, 10, 15, dan 20% berturut-turut sebesar 17,5; 19,8; 18,6; 23,7; dan 26,6 mm. Dari hasil penelitian menunjukkan ekstrak etanol kulit buah petai (Parkia speciosa Hassk.) memiliki aktivitas antijamur dengan terbentuknya zona hambat disekitar sumuran.
UJI AKTIVITAS SITOTOKSIK EKSTRAK DAN FRAKSI KULIT BUAH APEL MERAH (MALUS DOMESTICA (SUCKOW) BORKH.) TIPE RED DELICIOUS TERHADAP ARTEMIA FRANCISCANA KELLOGG DENGAN UJI BRINE SHRIMP LETHALITY TEST (BSLT) Novara Rahmania Zahwa; Ratu Choesrina; Siti Hazar
Bandung Conference Series: Pharmacy Vol. 3 No. 2 (2023): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v3i2.9143

Abstract

Abstract. Red apple skin contains flavonoid compounds that have anticancer potential. The plant is utilised as an anticancer drug. Red apple peel (Malus domestica (suckow) Borkh.) is thought to contain secondary metabolite compounds that can kill larvae and have cytotoxic activity. This study was conducted with the aim of knowing the characteristics of red apple peel and testing the cytotoxic activity of extracts and fractions of red apple peel (Malus domestica (suckow) Borkh.) in vitro using the Brine Shrimp Lethality Test (BSLT) method. The cytotoxic effect of each extract and fraction was identified against the percent mortality of Artemia franciscana Kellog larvae using testing concentrations of 10, 50, 100, 250, 500, 750, and 1000 ppm with the parameter of Lethal concentration 50 (LC50). The LC50 value of the extract was 71.614 ppm, the n-hexane fraction was 102.802 ppm, the ethyl acetate fraction was 127.350 ppm, and the water fraction was 143.879 ppm. The acquisition of the LC50 value in the four samples is included in the toxic range of less than 1000 ppm, where the acquisition of the LC50 value in the extract is more toxic than the fraction, with an LC50 value of 71.614 ppm. Due to the extract, there is a more synergistic combination of secondary metabolites. Abstrak. Kulit apel merah mengandung senyawa flavonoid yang berpotensi sebagai antikanker. Tumbuhan tersebut dimanfaatkan sebagai obat antikanker. Kulit buah apel merah (Malus domestica (suckow) Borkh.) diduga mengandung senyawa metabolit sekunder yang dapat membunuh larva dan memiliki aktivitas sitotoksik. Penelitian ini dilakukan dengan tujuan mengetahui karakteristik kulit buah apel merah serta menguji aktivitas sitotoksik dari ekstrak dan fraksi kulit buah apel merah (Malus domestica (suckow) Borkh.) secara in vitro menggunakan metode Brine Shrimp Lethality Test (BSLT). Efek sitotoksik dari masing-masing ekstrak dan fraksi diidentifikasi terhadap persen kematian larva Artemia franciscana Kellog menggunakan konsentrasi pengujian 10, 50, 100, 250, 500, 750 dan 1000 ppm dengan parameter nilai Lethal concentration 50 (LC50). Diperoleh nilai LC50 pada ekstrak sebesar 71,614 ppm, fraksi n-heksan 102,802 ppm, fraksi etil asetat sebesar 127,350 ppm sedangkan pada fraksi air sebesar 143,879 ppm. Perolehan nilai LC50 pada keempat sampel tersebut termasuk kedalam rentang toksik yaitu kurang dari 1000ppm dimana, perolehan nilai LC50 pada ekstrak lebih toksik dibandingkan fraksi, dengan nilai LC50 sebesar 71,614 ppm. Dikarenakan pada ekstrak terdapat kombinasi yang lebih sinergis antar metabolit sekunder.