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All Journal JURNAL PESISIR DAN LAUT TROPIS
Rosita AJ Lintang
FPIK UNSRAT
Earth & Planetary Sciences

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ISOLASI JAMUR SIMBION ASCIDIA Schizophyllum commune YANG MEMILIKI AKTIVITAS ANTIBAKTERI Gianfranco Montolalu; Deiske A Sumilat; Natalie D.C. Rumampuk; Inneke FM Rumengan; Rosita AJ Lintang; Renie Lucia Kreckhoff
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33569

Abstract

Ascidian is a benthic invertebrate that produces secondary metabolites. The substances were produced by the ascidian as self-defense from many environmental factors. Several studies report the similar structure between the substances extracted from ascidian and symbiotic fungi with biological activities. This study aims to isolate fungi associated with ascidian Eudistoma sp., then observe its potency on inhibiting bacteria S. aureus dan A. hydrophila. PDA was used to isolate and culture fungal isolate, then extraction using solvent ethyl acetate. Antibacterial activity of this symbiotic fungi isolates extract was performed using the Kirby-Bauer disc diffusion method. Molecular identification of fungi results that the symbiotic fungi were Schizophyllum commune. The observing result showed that fungal extract has the ability on inhibiting the growth of S. aureus and with the inhibition zone 8 mm and A. hydrophila 8,5 mm.Keywords: Ascidian, Eudistoma sp., Schizophyllum commune, Symbiotic, Antibacterial
PENAMBATAN MOLEKUL GLUTATION FAUNA LAUT TERHADAP RESEPTOR DARI BEBERAPA PENYAKIT VIRUS Q Xaxa Alvin Kai; Inneke FM Rumengan; Rosita AJ Lintang; Stenly Wullur; Deiske A Sumilat; Henneke Pangkey; Hein A Luntungan
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 2 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.2.2021.34853

Abstract

Glutathion is a tripeptides produced by almost all living creatures, including marine fauna such as rotifers and shrimps. Judging from the important molecular role of glutathion in living cell,  the ability of glutathion as antiviral compound, has been assed by application of the AutodockTools to predict the ability of glutathion as ligand to bind receptors of several viral diseases such as  covid-19, malaria, and hepatitis B. Docking results of glutathion toward target receptor were shown by the parameter, Gibbs energy value, and supported by other data such as the amount of hidrogen bond, amino acid residues, and bond distance. Evidently, the glutathion could bind to  each receptor of covid-19, malaria and hepatitis B, with values of  Gibbs energy (∆G), -7,9, 7,1 and -7,1 kcal/mol, respectively. This proves that glutathion has ability to bind covid-19 receptor, even stronger than its ability to bind receptors of other viral diseases, and to the remdesevir. Between glutathion and covid-19 receptor, there were 11 hidrogen bonds, with distance ranged from 2,76 to 3,25 Å. These were stronger than those between glutathion and receptors of malaria and hepatitis B, only  8 and 4 Å hidrogen bonds, respectively.  To develop glutathion as antiviral drug, it is necessary to carry out in vitro and in vivo analyses, prior to clinical test. Therefore, it requires to explore the marine fauna species that produce glutathion and their cultivation.Keywords: molecular docking, glutathion, marine fauna, autodock vina, viral diseases, binding affinity
KARAKTERISTIK MORFOLOGI DAN AKTIVITAS ENZIM PROTEASE BAKTERI SIMBION NUDIBRANCH Sabrina I Hengkengbala; Rosita AJ Lintang; Deiske A sumilat; Remy EP Mangindaan; Elvy Like Ginting; Sipriana Tumembouw
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36672

Abstract

One type of enzyme whose application is very widespread is protease enzymes. Microorganisms that have not been widely explored as a source of protease enzymes are bacteria that are in conjunction with Nudibranch. The study aims to identify the morphological characteristics of bacteria, then determine the hydrolysis ability of protease enzymes from bacteria symbiont Nudibranch. Identification of morphological characteristics is done macroscopic, and microscopic with gram staining. Proteolytic activity tests are performed qualitatively by measuring the proteolytic index of bacteria grown on Skim Milk Agar medium. The results of macroscopic identification of symbiotic bacteria with Nudibranch are dominated by irregular colony shapes, undulated edges, growth of the entire colony has a flat height, milky white colony color, buttery texture and opaque bacterial colonies. Based on microscopic identification, two gram-negative isolates are obtained, namely RL.S14, S1.1.1, and gram positive isolates, namely AK.S00, AK.S01, AK.S02, AK.S03, S.PP1, RL.S6, S2.1.5, S2.2.1, S3.3.1, S3.3.4, RL.S10. Nudibranch symbiont Bacterial has colony cells were dominated by the diplobacillus form. The results of the protease activity test of Nudibranch symbiont bacteria showed that 12 positive isolates had proteolytic activity with the highest proteolytic index produced by isolate AK.S00, which was 2.5 mm. Keywords: Nudibranch, symbiotic bacteria, characteristics, protease
Co-Authors Deiske A Sumilat Elvy Like Ginting Gianfranco Montolalu Hein A Luntungan Henneke Pangkey Inneke FM Rumengan Inneke FM Rumengan Natalie D.C. Rumampuk Q Xaxa Alvin Kai Remy EP Mangindaan Renie Lucia Kreckhoff Sabrina I Hengkengbala Sipriana Tumembouw Stenly Wullur