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All Journal JURNAL PESISIR DAN LAUT TROPIS
Deiske A Sumilat
FPIK UNSRAT
Earth & Planetary Sciences

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ISOLASI JAMUR SIMBION ASCIDIA Schizophyllum commune YANG MEMILIKI AKTIVITAS ANTIBAKTERI Gianfranco Montolalu; Deiske A Sumilat; Natalie D.C. Rumampuk; Inneke FM Rumengan; Rosita AJ Lintang; Renie Lucia Kreckhoff
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33569

Abstract

Ascidian is a benthic invertebrate that produces secondary metabolites. The substances were produced by the ascidian as self-defense from many environmental factors. Several studies report the similar structure between the substances extracted from ascidian and symbiotic fungi with biological activities. This study aims to isolate fungi associated with ascidian Eudistoma sp., then observe its potency on inhibiting bacteria S. aureus dan A. hydrophila. PDA was used to isolate and culture fungal isolate, then extraction using solvent ethyl acetate. Antibacterial activity of this symbiotic fungi isolates extract was performed using the Kirby-Bauer disc diffusion method. Molecular identification of fungi results that the symbiotic fungi were Schizophyllum commune. The observing result showed that fungal extract has the ability on inhibiting the growth of S. aureus and with the inhibition zone 8 mm and A. hydrophila 8,5 mm.Keywords: Ascidian, Eudistoma sp., Schizophyllum commune, Symbiotic, Antibacterial
PENAMBATAN MOLEKUL GLUTATION FAUNA LAUT TERHADAP RESEPTOR DARI BEBERAPA PENYAKIT VIRUS Q Xaxa Alvin Kai; Inneke FM Rumengan; Rosita AJ Lintang; Stenly Wullur; Deiske A Sumilat; Henneke Pangkey; Hein A Luntungan
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 2 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.2.2021.34853

Abstract

Glutathion is a tripeptides produced by almost all living creatures, including marine fauna such as rotifers and shrimps. Judging from the important molecular role of glutathion in living cell,  the ability of glutathion as antiviral compound, has been assed by application of the AutodockTools to predict the ability of glutathion as ligand to bind receptors of several viral diseases such as  covid-19, malaria, and hepatitis B. Docking results of glutathion toward target receptor were shown by the parameter, Gibbs energy value, and supported by other data such as the amount of hidrogen bond, amino acid residues, and bond distance. Evidently, the glutathion could bind to  each receptor of covid-19, malaria and hepatitis B, with values of  Gibbs energy (∆G), -7,9, 7,1 and -7,1 kcal/mol, respectively. This proves that glutathion has ability to bind covid-19 receptor, even stronger than its ability to bind receptors of other viral diseases, and to the remdesevir. Between glutathion and covid-19 receptor, there were 11 hidrogen bonds, with distance ranged from 2,76 to 3,25 Å. These were stronger than those between glutathion and receptors of malaria and hepatitis B, only  8 and 4 Å hidrogen bonds, respectively.  To develop glutathion as antiviral drug, it is necessary to carry out in vitro and in vivo analyses, prior to clinical test. Therefore, it requires to explore the marine fauna species that produce glutathion and their cultivation.Keywords: molecular docking, glutathion, marine fauna, autodock vina, viral diseases, binding affinity
POTENSI BIOAKTIVITAS ANTI JAMUR DAN ANTI-UV DARI ISOLAT JAMUR SIMBION PADA ASCIDIA Eudistoma sp Enjelika Polimba Sahuma; Deiske A Sumilat; Veibe Warow; Fitje Losung; Esther D Angkouw; Okstan Kalesaran
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36661

Abstract

Ascidia Eudistoma sp. produce bioactive compounds that can inhibit the growth of microorganisms. Ascidians are also known for their potential secondary metabolites in the biomedical world. This study aimed to obtain isolates of the ascidia symbiont Eudistoma sp., determine the antifungal activity against the test fungus Candida albicans, and determine the anti-UV activity of the symbiont Eudistoma sp. The ascidia symbiont fungus was extracted using maceration method with ethyl acetate solvent and tested for antifungal activity using the test fungus Candida albicans. The antifungal activity test results showed that the diameter of the AFBN 5b inhibition zone of 9 mm. The symbiont fungus can be said to have potential as an antifungal drug.  Testing the anti-UV activity of fungi in symbiosis with ascidia Eudistoma sp. using a spectrophotometer showed that the extract sample of the ascida symbiont fungus could produce an absorption at UV-B (λ 290–320 nm) of 3.8 absorbance and when compared to UV-A (λ 370–400 nm) an absorption value of 0.38 was obtained and is still classified as moderate Keywords: Ascidia, Eudistoma sp., symbionts, Antifungal and Anti-UV
KARAKTERISTIK MORFOLOGI DAN AKTIVITAS ENZIM PROTEASE BAKTERI SIMBION NUDIBRANCH Sabrina I Hengkengbala; Rosita AJ Lintang; Deiske A sumilat; Remy EP Mangindaan; Elvy Like Ginting; Sipriana Tumembouw
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36672

Abstract

One type of enzyme whose application is very widespread is protease enzymes. Microorganisms that have not been widely explored as a source of protease enzymes are bacteria that are in conjunction with Nudibranch. The study aims to identify the morphological characteristics of bacteria, then determine the hydrolysis ability of protease enzymes from bacteria symbiont Nudibranch. Identification of morphological characteristics is done macroscopic, and microscopic with gram staining. Proteolytic activity tests are performed qualitatively by measuring the proteolytic index of bacteria grown on Skim Milk Agar medium. The results of macroscopic identification of symbiotic bacteria with Nudibranch are dominated by irregular colony shapes, undulated edges, growth of the entire colony has a flat height, milky white colony color, buttery texture and opaque bacterial colonies. Based on microscopic identification, two gram-negative isolates are obtained, namely RL.S14, S1.1.1, and gram positive isolates, namely AK.S00, AK.S01, AK.S02, AK.S03, S.PP1, RL.S6, S2.1.5, S2.2.1, S3.3.1, S3.3.4, RL.S10. Nudibranch symbiont Bacterial has colony cells were dominated by the diplobacillus form. The results of the protease activity test of Nudibranch symbiont bacteria showed that 12 positive isolates had proteolytic activity with the highest proteolytic index produced by isolate AK.S00, which was 2.5 mm. Keywords: Nudibranch, symbiotic bacteria, characteristics, protease
UJI AKTIVITAS ANTIBAKTERI DAN ANTI-UV DARI EKSTRAK ETIL ASETAT ISOLAT JAMUR AFBK 5c YANG BERSIMBION DENGAN ASCIDIA Sigilina sp. DARI PERAIRAN PULAU BANGKA Vesiy NM Tamburian; Deiske A Sumilat; Chatrien A Sinjal
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 3 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36679

Abstract

Bioactive compounds are toxic compounds produced by organisms for use in self-defense from a wide variety of predators residing in their environment. The aim of the study was to test antibacterial activity and prove the UV-preventing effectiveness of the AFBK 5c mushroom isolate ethyl acetate extract that is in conjunction with ascidia. This research was conducted for 1 month in two locations, namely for antibacterial testing conducted in the Laboratory of Molecular Biology and Marine Pharmaceuticals, FPIK and for anti-UV testing conducted at the Pharmaceutical Laboratory, FMIPA, UNSRAT. The results showed that; This type of fungus that has been extracted and used is a fungus with the isolate code AFBK 5c This mushroom is a fungus that is connected with sigilina sp type ascidia. The rough extract of ascidia symbiont mushrooms is obtained from the process of meseration using ethyl acetate, then antibacterial testing of S. aureus and E. coli test bacteria and showing results such as antibacterial activity with an average value in S. aureus 09.00 and E. coli 11.05 mm which shows that there is antibacterial activity of AFBK 5c fungus isolate against E. coli and S. aureus test bacteria so that it can be said that the symbiont fungus has the potential as an ingredient. manufacture of antibacterial drugs. Furthermore, anti-UV testing, and the results of testing anti-UV compounds using rough extracts of AFBK 5c fungus isolates can produce absorption in UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm) so that it has the potential to be used as an anti-UV material.Keywords: Bioactive Compound, Ascidia, Meceration, Antibacterial, Anti-UV
Co-Authors Chatrien A Sinjal Christian Palit Elvy Like Ginting Enjelika Polimba Sahuma Esther D Angkouw Fitje Losung Gianfranco Montolalu Hein A Luntungan Henneke Pangkey Inneke FM Rumengan Inneke FM Rumengan Mutiara Ratih Natalie D.C. Rumampuk Okstan Kalesaran Putra Musak Q Xaxa Alvin Kai Remy EP Mangindaan Renie Lucia Kreckhoff Rosita AJ Lintang Sabrina I Hengkengbala Sipriana Tumembouw Stenly Wullur Veibe Warow Vesiy NM Tamburian