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All Journal BERITA BIOLOGI Aquacultura Indonesiana
Zafran, Zafran
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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EFEKTIVITAS KOMBINASI VAKSIN BAKTERI POLIVALEN DENGAN VAKSIN ANTI GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) [The Effectiveness of Polyvalent Bacterial Vaccine combined with Anti Grouper Sleepy Disease Iridovirus (GSDIV)Vaccine in Tiger Grouper (Epinephelus fuscoguttatus)] Zafran, Zafran
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i1.2862

Abstract

One problem in mariculture is mortality caused by diseases. An experiment to evaluate the effectiveness of polyvalent bacterial vaccine combined with anti GSDIV vaccine was conducted in the Institute for Mariculture Research and Development, Gondol, Bali. Polyvalent bacterial vaccine was combined of three inactivated pathogens, i.e. Vibrio harveyi, V. alginolyticus, and Photobacterium leioghnathi. Polyvalent bacterial vaccine administered through bathing method whereas GSDIV vaccine given through intra-peritoneal injection on tiger grouper. The fish was reared for three months in concrete tanks equipped with filter and aeration systems. The fish were fed with dry pellet twice a day. The results showed that titer antibody and survival rate of vaccinated fish were higher than unvaccinated one. The highest titer antibody was obtained on fish vaccinated with GSDIV vaccine (128) followed by fish vaccinated with polyvalent bacterial vaccine (32-64) and combination of polyvalent bacterial vaccine with GSDIV vaccine (32-64), respectively. Relative Percentage Survival (RPS) of vaccinated fish following challenge with live bacteria and virus were ranged from 65-84%. It is suggested that vaccines effective to enhance immune protection of tiger grouper fish against bacterial and viral infections.
Life Cycle of Marine Leech from Cultured Cantik Hybrid Grouper (Ephinephelus sp.) and Their Susceptibility Against Chemicals Murwantoko, Murwantoko; Condro, Sri Laksono; Isnansetyo, Alim; Zafran, Zafran
Aquacultura Indonesiana Vol 18, No 2 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.881 KB) | DOI: 10.21534/ai.v18i2.91

Abstract

Grouper is an important fish species due to high price both in domestic and international market. Several hybrid groupers have been released and can be accepted by farmers. A major production constraint in grouper culture is mortality due to disease. Leech is an ectoparasite for grouper which may cause significant loss, so that controlling this parasite is important as one of aquaculture management tools. The objectives of this study was to know the life cycle of leech on hybrid grouper and determine the efficacy of chemicals to kill leech under laboratory condition with diffferent dossage and immersing time. Life cycle was observed by reared an adult leech, counted the fecundity and observed the development of eggs until reach adult stages. The efficacy of formalin, albendazole, oxfendazole, levamisole, H2O2, CuSO4, ivermectin, vermizyn and freshwater on several concentration were assessed to kill adult leeches. All chemicals tested (except for freshwater), are dissolved in saline water salinity of 35 ppt at concentrations of 1000, 500, 250, 125 and 62.5 ppm. During 3 days of rearing, the adult leech could deposit of 11 eggs in average, with 600 µm – 800 µm in diameter. Twelve days were needed for the new egg inside the cocoon to hatch and develop into larvae under 24-25 °C at 34 ppt of salinity. It took another 9 days for the leeches larvae to grow reach mature stage. Five chemicals were  able to kill leeches (Zeylanicobdela arugamensis), which were fresh water, formalin, levamisol, ivermectin, and CuSO4. Treatment by exposure leech to freshwater for 30 minutes shows effective to kill leech.  Treatment with formalin with a dose of 250 ppm was able to kill leech after 1 hour immersion. 
Profile of Gonad Development and Spawn Milkfish Broodstock, Chanos chanos Forskall G-1 with Hormonal Implantation Reared in The Controlled Tank Dharma, Tony Setia; Zafran, Zafran
Aquacultura Indonesiana Vol 18, No 1 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.792 KB) | DOI: 10.21534/ai.v17i2.61

Abstract

This research was conducted in Institute for Mariculture Research and Development (IMRAD) using on concrete tanks 150 m³. The objective of this research was to know the performance and gonad maturations development and spawn activities of first generation (G-1) broodstock from individual selection. The experiment test of milkfish broodstock with weight size of 3,891.67±344.99 g were reared on tank. Fish were reared at initial density of 60 individuals fish in 150 m³ concrete tanks. The treatments were: with hormone (A) and without hormone (B) implantation. The doze of LHRH-a implant hormone was 50 µg/kg. The broodstock were reared using of standard procedure operational (SOP) for spawning and gonad maturations. The result of the experiment showed that fish with hormone implantation gave the better results in gonad development on stage of reproduction than fish without hormon implantation. The gonad maturations and development on male and female broodstock diameter of oocyte and sperm were 450 µm and positive 2 and 3, respectively. The broodstock was spawn on the continue rear culture in the tank. The frequency of spawning were 8 times (with hormone) and 4 times (without hormone implantation) with total number of egg was 1,660,000 and 500,000 pc, respectively. The quality of eggs were 55-95% for fertilities and index activity survival (SAI) after hatching larvae was 4-5 days.
Co-Authors ALIM ISNANSETYO Condro, Sri Laksono Murwantoko . Tony Setia Dharma