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All Journal Jurnal Akuakultur Indonesia Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Aquacultura Indonesiana SIGANUS: Journal of Fisheries and Marine Science Journal of Multidisciplinary Applied Natural Science
ALIM ISNANSETYO
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Growth, Total Production and Feed Efficiency of Catfish (Clarias sp.) Orally Administered with Shrimp Waste Hydrolyzate Hariyadi, Dimas Rizky; Isnansetyo, Alim; Istiqomah, Indah; Hardaningsih, Ign.; Wahyudi, Wahyudi; Kim, Sung Sam
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.821 KB) | DOI: 10.21534/ai.v19i1.116

Abstract

Shrimp Waste Hydrolyzate (SWH) /Shrimp Soluble Extract (SSE) is a product produced from the enzymatic process of shrimp waste. SWH contains essential and non-essential amino acids which useful for fish. This study aimed to determine effect of SWH on the growth and total production of catfish. The parameters observed were average growth rate, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), total production and length weight relationship from beginning until the end of rearing period. Growth performance was observed by calculating average growth rate and SGR of catfish. The results of this study showed that oral administration of SWH significantly increased SGR of catfish length at 4th sampling period with value 1.54% body length/day. Administration of SWH did not affect on the AGR, FCR, PER and total production. The growth pattern of catfish is negative allometric.
Oral Administration of Alginate From A Tropical Brown Seaweed, Sargassum sp. To Enhance Non-Spesific Defense In Walking Catfish (Clarias sp.) Isnansetyo, Alim; Irpani, Husni Mubarok; Wulansari, Tyas Ayu; Kasanah, Noer
Aquacultura Indonesiana Vol 15, No 1 (2014): Volume 15 Issue 1 Year 2014
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.095 KB) | DOI: 10.21534/ai.v15i1.29

Abstract

Alginate that is usually extracted from brown seaweeds, is a bioactive substance with immunomodulator activity. The purpose of this research was to evaluate the effect of oral administration of alginate on the non-specific immune system of walking catfish (Clarias sp.). An experiment was conducted in a Completely Randomized Design with five treatments in triplicates. Alginate was supplemented in feed at doses of 0 (control treatment), 2, 4, 6 and 8 g/kg of feed. The walking catfish was fed twice daily at feeding rate of 5%. Non-specific defense parameters evaluated were Nitroblue Tetrazolium (NBT) activity, phagocytic activity (PA), phagocytic index (PI), leukocyte differentiation, hematocrit and leucocrit. The parameters were observed prior to be treated, 5th, 10th and 15th days after treatments. Oral administration of alginate at 4 g/kg of feed increased NBT and PA activities significantly (P<0.05) in 5 days, while alginate at a dose of 6 g/kg increased NBT activity and monocyte percentage significantly (P<0.05) in 5 days. These results suggested that oral administration of alginate at 4 g/kg effectively increased non-specific immune system of walking catfish.
Life Cycle of Marine Leech from Cultured Cantik Hybrid Grouper (Ephinephelus sp.) and Their Susceptibility Against Chemicals Murwantoko, Murwantoko; Condro, Sri Laksono; Isnansetyo, Alim; Zafran, Zafran
Aquacultura Indonesiana Vol 18, No 2 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.881 KB) | DOI: 10.21534/ai.v18i2.91

Abstract

Grouper is an important fish species due to high price both in domestic and international market. Several hybrid groupers have been released and can be accepted by farmers. A major production constraint in grouper culture is mortality due to disease. Leech is an ectoparasite for grouper which may cause significant loss, so that controlling this parasite is important as one of aquaculture management tools. The objectives of this study was to know the life cycle of leech on hybrid grouper and determine the efficacy of chemicals to kill leech under laboratory condition with diffferent dossage and immersing time. Life cycle was observed by reared an adult leech, counted the fecundity and observed the development of eggs until reach adult stages. The efficacy of formalin, albendazole, oxfendazole, levamisole, H2O2, CuSO4, ivermectin, vermizyn and freshwater on several concentration were assessed to kill adult leeches. All chemicals tested (except for freshwater), are dissolved in saline water salinity of 35 ppt at concentrations of 1000, 500, 250, 125 and 62.5 ppm. During 3 days of rearing, the adult leech could deposit of 11 eggs in average, with 600 µm – 800 µm in diameter. Twelve days were needed for the new egg inside the cocoon to hatch and develop into larvae under 24-25 °C at 34 ppt of salinity. It took another 9 days for the leeches larvae to grow reach mature stage. Five chemicals were  able to kill leeches (Zeylanicobdela arugamensis), which were fresh water, formalin, levamisol, ivermectin, and CuSO4. Treatment by exposure leech to freshwater for 30 minutes shows effective to kill leech.  Treatment with formalin with a dose of 250 ppm was able to kill leech after 1 hour immersion. 
Short-term Response in Molecular and Biochemical Adaptation of White Shrimp (Litopenaeus vannamei) Postlarvae Reared in A Biofloc System Yasa, Ngurah Sedana; Anshory, Lutfi; Triastutik, Gemi; Murwantoko, Murwantoko; Isnansetyo, Alim; Lusiana, Lusiana
Aquacultura Indonesiana Vol 20, No 2 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.262 KB) | DOI: 10.21534/ai.v20i2.155

Abstract

Up regulation of heat shock proteins (HSPs) in organisms can be detected in response to many kinds of stressor. Recently there were few studies have focused on the three kinds of heat shock proteins (HSPs) and antioxidant enzyme activity after biofloc stress. The objective of this study was to investigate the effect of different biofloc volume on the expressions of (LvHSP60, LvHSP70, and LvHSP90), the activity of superoxide dismutase (SOD) and phenoloxidase (PO) of the L. vannamei after biofloc addition. PL10 L. vannamei were divided into three groups: precondition, biofloc stress (5mL/L, 10mL/L, 15mL/L), and recovery. The gene expression results showed that the expressional levels of LvHSP60, LvHSP70, and LvHSP90 were increased significantly in 6h treatment and tend return to normal conditions after 48h treatment. Superoxide dismutase activity was reduced during treatment and phenoloxidase activity was elevated slightly after 12h to 24h and tend to pretreatment level at recovery periods. All of these HSPs expression reverted to normal levels 6h after the recovery period. The results indicated that different expression patterns of the three HSPs. HSP60 have a longer and higher protection expression after 12h treatment than HSP70 and HSP90. HSP90 was more sensitive in 6h at all treatments than HSP60 and HSP70. It is concluded that supplementation of biofloc with the volume of 5-15mL/L caused Hsp protection in L. vannamei PLs at the first 6h to 48h treatment and increased phenoloxidase activity at 24-48h treatment and reduced survival rate of the white shrimp. 
COMPARISON OF NUTRITIONAL AND PROTEASE ACTIVITY PROFILES OF TWO LIVE FEED CANDIDATES OF Pseudodiaptomus SPECIES Rina Puji Astuti; Alim Isnansetyo; Rarastoeti Pratiwi; Suwarno Hadisusanto
Indonesian Aquaculture Journal Vol 17, No 1 (2022): (June, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.1.2022.45-51

Abstract

Pseudodiaptomus species are one of the copepods species as a superior live feed to date due to their nutrition and digestive enzyme contents. Some of them have been used as natural for rearing marine fish larvae. The purposes of this study were to compare the nutritional and protease activity between two species of Pseudodiaptomus originated from Indonesian waters, and to determine more superior species to cultivate. Two different feeds i.e. Thalassiosira sp. and milk powder were used to grow the Pseudodiaptomus species. Analysis of amino acids (AAs) and fatty acids (FAs) profiles were carried out for both the Pseudodiaptomus species samples and the feeds, while the protease activity assay was carried out only for the Pseudodiaptomus species samples. Results indicated that the nutritional and protease activity profiles of Pseudodiaptomus were affected by the types of feed. Pseudodiaptomus code P61 was more superior to Pseudodiaptomus code P71. This code P61 species contained a wide variety of essential fatty acids and exhibited stabile protease activity under the different feeding treatments. However, P61 contained a lower total AA content than P71. Both of them could be cultivated because they were complementary in nutrients to each other.
FEED EFFICIENCY AND GROWTH OF CATFISH (Clarias sp.) FED WITH THE ADDITION OF IMMUNE-BOOSTING FERMENTED EARTHWORMS Mukhlisnah Djalil; Alim Isnansetyo; Triyanto Triyanto; Tito Arya Nugraha
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.157-163

Abstract

A fermented earthworm (FEW) is reported to be an alternative to an immune-modulator feed additive in catfish. However, the effects on growth and feed efficiency have not been reported yet. The present study aimed to evaluate the effects of fermented earthworms (FEW) on the growth and feed efficiency of catfish (Clarias sp.). A feeding trial was conducted in a completely randomized design with five treatments of diet in quadruplicate. The trial was conducted for 78 days. The observed parameters comprised of survival rate (SR), specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), protein efficiency ratio (PER), and total biomass. The results revealed that the FEW at up to 5% did not affect (P>0.05) the growth rate of catfish, but FEW gave a negative effect on the growth rate (P>0.05) at the addition rate higher than 5%. The highest growth rate was found at the addition rate of 2.5%. FEW also did not affect the survival rate (SR), FCR, PER, and total biomass (P>0.05). This study successfully confirmed that FEW could be used as an alternative to immuno-modulator ingredient without any negative impact on the growth of catfish when FEW was added to the feed at as high as 5%. These findings give a new perpective in utilizing FEW as a fuctional aqua-feed ingredient to increase immune response without alteration of the fish growth.
Toksisitas Daun Miana (Coleus scutellarioides (L) Benth) Sebagai Antibakteri Pada Udang Vannamei (Litopenaeus vannamei) Basir, Buana; Isnansetyo, Alim; Istiqomah, Indah; Jabbar, Firmansyah Bin Abd
SIGANUS: Journal of Fisheries and Marine Science Vol 1 No 2 (2020): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v1i2.653

Abstract

Tujuan Penelitian ini untuk menganalisis toksisitas daun miana sebagai antibakteri pada udang vanamei. Penelitian ini dilaksanakan pada Maret sampai Oktober 2019, di Laboratorium Sekolah Tinggi Teknologi Kelautan (STITEK) Makassar. Sampel daun miana berasal dari beberapa daerah di Sulawesi Selatan. Uji aktivitas antibakteri ekstrak daun miana dengan tiga fraksi, yaitu ethanol, n-hexane, dan chloroform menggunakan metode difusi kertas cakram dengan konsentrasi ekstrak 500 µg/mL dan 1000 µg/mL, sebanyak tiga kali ulangan. Uji toksisitas metode BST (LC70) dengan konsentrasi uji 2500, 1250, 625, 312.5, 156.25, 78.125 µg/mL. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa uji aktivitas ekstrak daun miana tertinggi pada fraksi chloroform, dengan diameter hambat 28 mm. Sementara pada antibiotik oxytetracycline diameter hambat sebesar 31 mm. Nilai toksisitas pada tingkat mortalitas 30% pada konsentrasi 1250 µg/mL. Ekstrak daun miana fraksi chloroform mampu menggantikan peran antibiotic oxytetracycline dengan penggunaan konsentrasi dibawah 1250 µg/mL.
Agarolytic Bacillus sp. FRAgK1 Screened from Gracilaria (Rhodophyta) Thallus as Probiotic Candidate for Abalone Abdullah, Faisal Zain; Istiqomah, Indah; Isnansetyo, Alim; Yasa, Ngurah Sedana; Ismail, Norshida
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.93662

Abstract

Agarolytic bacteria produce agarase, which may aid in the growth of cultured tropical abalone fed natural seaweed. Agarolytic bacteria can come from a variety of sources, such as seawater, abalone intestines, and dead seaweed. This study aimed to isolate, screen, describe, and identify agarolytic bacteria found in red macroalgae. Agarolytic bacteria isolated from Gracillaria segregated from the substrate at Drini Beach, Gunungkidul Regency, were qualitatively described using the agarolytic index, antibiotic susceptibility, acid resistance (pH 4), and safety test. We collected seven agarolytic isolates. FRAgK1 isolate had the highest agarolytic index, was sensitive to antibiotics, resistant to low pH conditions, and non-pathogenic to finfish, making it suitable for use as gut probiotics in abalone. The bacterium was short rod-shaped, Gram-positive, non-motile, lacked catalase and indol, and was unable to ferment lactose or sucrose. The 16S rRNA gene sequence of FRAgK1 was most similar to Bacillus subtilis, however only by 99.43%.
Pathogenicity test bacteria from Oreochromis niloticus and Clarias gariepinus aquaculture ponds Hardi, Esti Handayani; Halim, Aldi M.; Nugroho, Rudy Agung; Mawardi, Mira; Isnansetyo, Alim; Mariana Lusiastuti, Angela; Rahayu, Widianingsih; Niklani, Andi; Saptiani, Gina
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.97-105

Abstract

Present research aimed to examine the pathogenicity opportunities of some bacteria which were isolated from freshwater aquaculture ponds to some fish Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius. The bacteria were isolated from the water and feces of O. niloticus and C. gariepinus aquaculture in Samarinda, which were identified as Escherichia coli, Enterobacter cloacae, and Enterobacter amnigenus. An 0.1 mL-1 of each bacteria was intramuscular injection and evaluated the clinical signs, eksternal pathology, and fish mortality. The freshwater fish using in this research were Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius with size 10-15 g from Loa Kulu, Kutai Kartanegara Regency, East Kalimantan, Indonesia. The result showed that each bacteria caused mortality in fish: E. coli bacteria caused fish mortality 23.33-66.67%; E. cloacae 10.00 to 90.00%; and E. amnigenus by 3.33-56.67%. The average death time of E. coli and E. amnigenus in all three fish were under 48 hours, while E. cloacae caused mortality less than 48 hours in C. gariepinus and P. pangasius. In conclusion, E. coli, E. cloacae, and E. amnigenus are putative pathogenic bacterium in O. niloticus, C. gariepinus, and P. pangasius.
In Vitro Anti-Acne Activity and Bioactive Compound Analysis of Sargassum cristaefolium Ethanolic Extract from Teluk Awur Jepara Kristiani, Theresia Adven Dea; Husni, Amir; Isnansetyo, Alim
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.262

Abstract

Acne is a prevalent skin disorder, particularly among adolescents, causing significant physical and psychological impacts. Ongoing efforts are dedicated to acne treatment by developing therapeutic agents with enhanced effectiveness while minimizing side effects. In this study, we aimed to assess the potential of Sargassum cristaefolium sourced from Teluk Awur Jepara, extracted using ethanol, in combating acne through its in vitro antibacterial and antioxidant attributes. Utilizing the disk diffusion method, our findings demonstrated the extract's efficacy against Cutibacterium acne, Staphylococcus epidermidis, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration were determined to be 50 and >100 mg mL-1 against C. acnes, 25 and 100 mg mL-1 against S. epidermidis, and 25 and 100 mg mL-1 against S. aureus, respectively. Additionally, bacteriolytic assays confirmed the extract's ability to partially lyse bacterial cells, particularly S. aureus and S. epidermidis at 2× MIC, while C. acne exhibited partial lysis until the 6th hour, followed by an increase in absorbance, possibly due to cellular debris aggregation or bacterial persistence due to incomplete lysis. Furthermore, the extract exhibited notable free radical scavenging properties against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), with IC50 values of 645.35 ± 9.52 and 574 ± 33.52 µg mL-1, respectively. The S. cristaefolium ethanol extract exhibited a total phenol content of 14.17 ± 0.39 mg GAE g-1 and a sulfate content of 10.99 ± 0.45%. Liquid chromatography-mass spectrometry (LC-MS) analysis identified bioactive compounds, including carotenoids, terpenoids, steroids, flavonoids, chromenols, and fatty acids, all of which demonstrate substantial potential as antioxidants and antibacterial agents against acne-causing bacteria. Therefore, ethanolic extract of S. cristaefolium demonstrates potential as an anti-acne treatment due to its antibacterial and antioxidant properties.
Co-Authors Abdullah, Faisal Zain Amir Husni Angela Mariana Lusiastuti Anshory, Lutfi Buana Basir Condro, Sri Laksono Esti Handayani Hardi Gina Saptiani Halim, Aldi M. Hariyadi, Dimas Rizky Ign. Hardaningsih INDAH ISTIQOMAH Irpani, Husni Mubarok Ismail, Norshida Jabbar, Firmansyah Bin Abd Kim, Sung Sam Kristiani, Theresia Adven Dea Lusiana, Lusiana Mira Mawardi Mukhlisnah Djalil Murwantoko . Niklani, Andi Noer Kasanah Putra, Hemi Adi Rahayu, Widianingsih Rarastoeti Pratiwi Rina Puji Astuti Rudy Agung Nugroho SUWARNO HADISUSANTO Tito Arya Nugraha Triastutik, Gemi Triyanto Triyanto Wahyudi Wahyudi Wulansari, Tyas Ayu Yasa, Ngurah Sedana Yasa, Ngurah Sedana Zafran, Zafran