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All Journal Majalah Kedokteran Gigi Indonesia
Jansen, John
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Dentistry

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A histological evaluation of cellular response on bone regeneration scaffold Dewi, Anne Handrini; Listyarifah, Dyah; Ardhani, Retno; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77449

Abstract

Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
Anti-inflammatory loading of cinnamaldehyde on artificial bone scaffolds against the process of bone regeneration Dewi, Anne Handrini; Triawan, Andi; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.87347

Abstract

Cinnamaldehyde is an organic component in cinnamon and has anti-inflammatory, antibacterial, and osteogenic properties. Loading cinnamaldehyde with a concentration of 4% into an artificial scaffold from gypsum calcium carbonate hydrogel could reduce inflammation, accelerate healing, and promote new bone regeneration at bone implantation surgery. The aim of this study was to investigate the impact of cinnamaldehyde loaded on gypsum-hydrogel calcium carbonate on the process of wound healing and bone regeneration in rats. Implantation of bone scaffolding from gypsum hydrogel calcium carbonate with an addition of cinnamaldehyde was performed on 20 5-month-old Sprague-Dawley rats weighing 300-350 g in an artificial defect of condyle femoris bone dexter and sinister. Group A was a control with gypsum only, while groups B, C, and D were given gypsum and CaCO3 with a combination of cinnamaldehyde or dehydrothermal treatment (DHT). Euthanasia was performed after implantation at 1, 4 and 8 weeks for 4 groups (n = 3). Femoris condyle bone was cut, made into histological preparations by hematoxylin eosin (HE) staining, and analyzed descriptively. The results showed that the loading of cinnamaldehyde on the scaffold was effective in weeks 1 and 4, but after 8 weeks of implantation, cinnamaldehyde was observed to inhibit defect closure. The cinnamaldehyde group combined with dehydrothermal (DHT) treatment was found to be better than those without DHT.
Co-Authors Andi Triawan, Andi Anne Handrini Dewi Dyah Listyarifah Retno Ardhani