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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) The Indonesian Journal of Dental Research MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement)
Dyah Listyarifah
Department Of Dental Biomedical Sciences, Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Decision Sciences, Operations Research & Management Dentistry Health Professions Immunology & microbiology Neuroscience

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Histocompatibility evaluation of combination of gypsum with carbonated hydroxyapatite as bone substitutes in subcutaneous tissue Ika Dewi Ana, Dyah Listyarifah Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.98 KB)

Abstract

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Softtissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contactwith the soft tissues upon application and can cause severe inflammatory response. The aim of this study was toevaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks ofgypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histologicalsection were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for softtissueimplants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidenceinterval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on day 7, 14, and21. Moreover, there was no significant difference (p>0.05) of histomorphometrics score between both implants ineach implantation periods. It could be concluded that histocompatibility level of gypsum and combination of gypsum-CHA to soft tissue was the same until day 21 iof mplantation.Key words: gypsum - carbonated hydroxyapatite – biocompatibility – histocompatibility - subcutaneous implant
Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk; Utami, Trianna Wahyu; Dewi, Anne Handrini; Listyarifah, Dyah; Ruspita, Intan; Nur, Asikin; Susilowati, Heni
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

Abstract

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.
Remodeling Capacity of Femoral Bone Defect by POP-CHA Bone Substitute: A Study in Rats’ Osteoclast (First Series of POP-based Bone Graft Improvement) Steven Kumar; Anne Handrini Dewi; Dyah Listyarifah; Ika Dewi Ana
The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1340.876 KB) | DOI: 10.22146/theindjdentres.10008

Abstract

Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malformations can be very difficult to perform. Bone engineering offers an option to improve bone reconstruction procedures. This interdisciplinary field applies the principles of biology and engineering to the development of functional substitutes for damaged bone. Our research aimed to find the ideal scaffold for bone regeneration, focusing on Calcium and Phosphate combination. In this study, Plaster of Paris (POP) was combined with CHA and implanted in femoral condyles of rats. According to the experimental result, it can be concluded that there was no significant difference in response to the implantation of POP and POP-CHA in Sprague Dawley rat femur condyle (p<0.05). It can be stated that both POP-CHA and POP shows similar trait in bone healing.
Dealing with the high-risk potential of COVID-19 cross-infection in dental practice Heribertus Dedy Kusuma Yulianto; Nunuk Purwanti; Trianna Wahyu Utami; Anne Handrini Dewi; Dyah Listyarifah; Intan Ruspita; Asikin Nur; Heni Susilowati
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.56588

Abstract

The World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection.
Histocompatibility evaluation of combination of gypsum with carbonated hydroxyapatite as bone substitutes in subcutaneous tissue Dyah Listyarifah Rina Susilowati Ika Dewi Ana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.98 KB)

Abstract

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Softtissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contactwith the soft tissues upon application and can cause severe inflammatory response. The aim of this study was toevaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks ofgypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histologicalsection were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for softtissueimplants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidenceinterval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on day 7, 14, and21. Moreover, there was no significant difference (p>0.05) of histomorphometrics score between both implants ineach implantation periods. It could be concluded that histocompatibility level of gypsum and combination of gypsum-CHA to soft tissue was the same until day 21 iof mplantation.Key words: gypsum - carbonated hydroxyapatite – biocompatibility – histocompatibility - subcutaneous implant
Kompatibilitas bahan implan tulang hidroksiapatit dan karbonat hidroksiapatit di jaringan lunak Dyah Listyarifah; Gloria Fortuna; Ryan Christian Pramuditya; Anne Handrini Dewi; Retno Ardhani
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 8, No 3 (2022)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.83547

Abstract

Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih baik daripada hidroksiapatit (HA). Secara in vivo, CHA lebih mudah larut daripada HA serta dapat meningkatkan konsentrasi lokal ion kalsium dan fosfat yang diperlukan untuk proses pembentukan jaringan tulang baru. Tes biokompatibilitas jaringan lunak diperlukan untuk setiap bahan implan karena ketika diaplikasikan ke dalam tubuh yang akan kontak dengan jaringan lunak. Penelitian ini bertujuan untuk mengevaluasi biokompatibiltas CHA dan HA di jaringan lunak. CHA dan HA ditanamkan pada jaringan subkutan paravertebral dari 12 tikus Wistar jantan. Irisan histologis diwarnai dengan Hematoksilin Eosin. Kuantitas dan kualitas kapsul fibrosa serta jumlah sel raksasa benda asing (foreign body giant cells, FBGCs) dan nukleinya dievaluasi. Perbedaan modus kualitas dan kuantitas kapsul antara periode implantasi dianalisis dengan Kruskall-Wallis, sedangkan perbedaan modus antara bahan implan pada periode yang sama dianalisis dengan uji Wilcoxon. Rata-rata FBGCs dinilai dengan two-way ANOVA dengan interval kepercayaan 95% diikuti dengan uji-t berpasangan untuk menganalisis perbedaan rata-rata antara bahan dan uji-t sampel independen untuk menganalisis perbedaan rata-rata antara periode implantasi. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan dalam kuantitas dan kualitas kapsul setelah implantasi CHA dan HA pada periode yang sama dan antar periode (p ≥ 0,05). Jumlah FBGCs di CHA setelah 14, 21, 28 hari implantasi secara signifikan lebih rendah dibandingkan dengan HA, akan tetapi tidak ada perbedaan yang signifikan dalam jumlah nuklei FBGC antara implantasi HA dan CHA. Kesimpulan studi ini menunjukkan bahwa CHA lebih biokompatibel daripada HA, terutama pada fase subkronik.
A histological evaluation of cellular response on bone regeneration scaffold Dewi, Anne Handrini; Listyarifah, Dyah; Ardhani, Retno; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77449

Abstract

Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
Comparison Effect of Leaves and Bark Extract of Eucalyptus (Melaleuca leucadendra), Sappan (Caesalpinia sappan), and Cinnamon (Cinnamomum zeylanicum) to Reduce Streptococcus mutans Biofilm Formation Utami, Trianna Wahyu; Ekananda, Bernadetha Nathania; Anjani, Yasmin Regita; Listyarifah, Dyah; Nur, Asikin; Syahbudin, Atus
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.83283

Abstract

To evaluate the effect of eucalyptus, sappan, and cinnamon leaf and bark extract on the percentage of S. mutans biofilm formation. The test group was divided into a negative control (1% Dimethyl sulfoxide), a treatment group (eucalyptus, sappan, and cinnamon leaves and bark at concentrations of 50%, 25%, and 12.5%, respectively), and a positive control (0.2% chlorhexidine). Each concentration of eucalyptus, sappan, and cinnamon leaves and barks extract, Brain Heart Infusion Broth (BHI-B), bacteria according to the McFarland 0.5 standard, a positive control, and a negative control were added to a 96-well microplate. They were incubated at 37 °C for twenty-four hours before being rinsed with phosphate buffer saline (PBS) and stained with 0.1% crystal violet. The optical density was then measured using a microplate reader with a 540 nm wavelength. The absorbance value is then factored into the percentage of bacterial adhesion inhibition formula. A statistical test revealed a significant difference (p < 0.05) in the percentage of inhibition between the three extracts of leaves and bark and the negative control group (1% DMSO). There is a significant difference between all leaves and barks concentration and the positive control group except the 50% concentration of sappan leaf group and 50% concentration of cinnamon leaf group. The sappan leaf and cinnamon leaf at 50% concentration exhibit no significant difference (p > 0.05) with the positive control. Eucalyptus, sappan, cinnamon leaves and barks at a concentration of 50% demonstrated effectiveness of the extractant in inhibiting the formation of biofilm masses by S. mutans in comparison with the other group.
Rehabilitation of Toothless Conditions with Dental Implants to Improve the Nutritional Status and General Health of the Terban Village Community Rosanto, Yosaphat Bayu; Listyarifah, Dyah; Budi Nagari, Hanni Handayani
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 1 (2024): March
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.90557

Abstract

Terban Village is a village that has a significant number of residents who suffer from toothless conditions. This condition directly affects the village population’s nutritional status and general health. This community service aimed to rehabilitate the condition of toothlessness with dental implants to improve the nutritional status and general health of the people of Terban Village. The methods used in this community service were counseling, general health, mental and dental examinations, health utility levels, radiological examinations, and dental implant treatment for the residents. The output of this community service was a counseling session that involved 70 Terban Village residents. The DMFT index of Terban Village residents was 7.47 and was still above the WHO baseline. Five respondents were selected to undergo rehabilitation procedures with dental implants. Rehabilitation of edentulous conditions with dental implants will have a positive impact on improving the nutritional status and general health of the people of Terban Village. In addition, the general health of the population has also experienced improvements, such as reducing the number of cases of nutrition-related diseases and improving the overall quality of life.
Co-Authors Anjani, Yasmin Regita Anne Handrini Dewi Anne Handrini Dewi Asikin Nur Atus Syahbudin Budi Nagari, Hanni Handayani Ekananda, Bernadetha Nathania Gloria Fortuna Heni Susilowati Heni Susilowati Heribertus Dedy Kusuma Yulianto Ika Dewi Ana Intan Ruspita Intan Ruspita, Intan Jansen, John Nunuk Purwanti Retno Ardhani Ryan Christian Pramuditya Steven Kumar Trianna Wahyu Utami Yosaphat Bayu Rosanto