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All Journal Jurnal Kebijakan Kesehatan Indonesia Jurnal Teknosains Indonesian Journal of Cancer Chemoprevention MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi)
Retno Ardhani
Department Of Dental Biomedical Sciences, Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Health Professions Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Public Health

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SERI METHODS IN MOLECULAR BIOLOGY Retno Ardhani
Jurnal Teknosains Vol 7, No 1 (2017): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.36989

Abstract

Sejarah telah mencatat andil penelitian dalam menciptakan kehidupan yang lebih baik. Salah satunya penemuan antibiotik pada 1940an yang diawali dari pengamatan Alexander Fleming pada 1928 terhadap fenomena kematian bakteri Staphylococcus dalam cawan petri yang juga ditumbuhi jamur. Sebelum ditemukan antibiotik, tidak ada obat untuk pneumonia, gonorrhea atau demam rematik sehingga rumah sakit dipenuhi pasien sedangkan tidak banyak yang dapat dilakukan dokter untuk menolong. Tidak mengherankan jika kemudian Alexander Fleming bersama Howard Florey, Ernst Chain yang merupakan tim penemu antibiotik penicillin, dianugerahi Nobel Prize untuk Fisiologi dan Kedokteran pada 1945.
Konvergensi Kebijakan Riset dan Inovasi untuk Resiliensi Industri Alat Kesehatan di Indonesia Hargo Utomo; Dian Kesumapramudya Nurputra; Retno Ardhani
Jurnal Kebijakan Kesehatan Indonesia Vol 12, No 1 (2023)
Publisher : Center for Health Policy and Management

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkki.81408

Abstract

Latar Belakang: Konvergensi kebijakan riset dan inovasi diharapkan menjadi katalisator resiliensi melalui penguatan kapasitas inovasi industri dan relasi kebaruan pengetahuan penyokong inovasi. Tujuan: Studi kebijakan ini bertujuan menjawab pertanyaan tentang bagaimana riset dan inovasi di Indonesia bergerak sejalan dengan arah kebutuhan resiliensi industri alat kesehatan di era perubahan. Metode: Pendekatan deskriptif-analitik dipergunakan untuk mengeksplorasi fenomena faktual dan menjawab pertanyaan penelitian. Data primer dikumpulkan melalui observasi lapang ke pelaku industri alat kesehatan dan Focus Group Discussion yang melibatkan sejumlah pemangku kepentingan. Data sekunder dari berbagai bersumber selanjutnya dipergunakan untuk memperkuat argumen dalam analisis kebijakan. Hasil: Hasil studi menyimpulkan bahwa ketahanan atau resiliensi industri alat kesehatan ditentukan oleh faktor penguatan kapasitas inovasi dan keterkaitan pengetahuan baru untuk pemunculan inovasi. Kesimpulan: Kebijakan afirmatif ditempuh sebagai opsi keberpihakan terhadap ketahanan industri melalui kesinambungan rantai pasok, penyebaran inovasi, dan keterjangkauan hasil inovasi industri bagi masyarakat.
Kompatibilitas bahan implan tulang hidroksiapatit dan karbonat hidroksiapatit di jaringan lunak Dyah Listyarifah; Gloria Fortuna; Ryan Christian Pramuditya; Anne Handrini Dewi; Retno Ardhani
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 8, No 3 (2022)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.83547

Abstract

Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih baik daripada hidroksiapatit (HA). Secara in vivo, CHA lebih mudah larut daripada HA serta dapat meningkatkan konsentrasi lokal ion kalsium dan fosfat yang diperlukan untuk proses pembentukan jaringan tulang baru. Tes biokompatibilitas jaringan lunak diperlukan untuk setiap bahan implan karena ketika diaplikasikan ke dalam tubuh yang akan kontak dengan jaringan lunak. Penelitian ini bertujuan untuk mengevaluasi biokompatibiltas CHA dan HA di jaringan lunak. CHA dan HA ditanamkan pada jaringan subkutan paravertebral dari 12 tikus Wistar jantan. Irisan histologis diwarnai dengan Hematoksilin Eosin. Kuantitas dan kualitas kapsul fibrosa serta jumlah sel raksasa benda asing (foreign body giant cells, FBGCs) dan nukleinya dievaluasi. Perbedaan modus kualitas dan kuantitas kapsul antara periode implantasi dianalisis dengan Kruskall-Wallis, sedangkan perbedaan modus antara bahan implan pada periode yang sama dianalisis dengan uji Wilcoxon. Rata-rata FBGCs dinilai dengan two-way ANOVA dengan interval kepercayaan 95% diikuti dengan uji-t berpasangan untuk menganalisis perbedaan rata-rata antara bahan dan uji-t sampel independen untuk menganalisis perbedaan rata-rata antara periode implantasi. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan dalam kuantitas dan kualitas kapsul setelah implantasi CHA dan HA pada periode yang sama dan antar periode (p ≥ 0,05). Jumlah FBGCs di CHA setelah 14, 21, 28 hari implantasi secara signifikan lebih rendah dibandingkan dengan HA, akan tetapi tidak ada perbedaan yang signifikan dalam jumlah nuklei FBGC antara implantasi HA dan CHA. Kesimpulan studi ini menunjukkan bahwa CHA lebih biokompatibel daripada HA, terutama pada fase subkronik.
A histological evaluation of cellular response on bone regeneration scaffold Dewi, Anne Handrini; Listyarifah, Dyah; Ardhani, Retno; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77449

Abstract

Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
Confirmation of the potential mechanism of pentagamavunon-0 against temporomandibular arthritis using bioinformatic approaches Robin, Dwi Merry Christmarini; Ardhani, Retno; Novitasari, Dhania; Kusumawardani, Banun; Aulia Rahman, Faaza; Meiyanto, Edy; Purwanti, Nunuk
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 4 (2025): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i4.p367-375

Abstract

Background: Non-steroidal anti-inflammatory drugs (NSAID) are widely used in temporomandibular joint osteoarthritis management. However, the side effects of NSAIDs on multiple organs need to be anticipated. Curcumin is known for its anti-inflammatory and analgesic potential, comparable to that of NSAIDs. In a previous study, structurally modified curcumin increased the pharmacological effect and simultaneously reduced the toxicity and side effects of curcumin. Pentagamavunon-0 (PGV-0) is one of the active components synthesized by the structure modification of curcumin. Purpose: In this study, we identify the potential target of PGV-0 on the pathogenesis of temporomandibular arthritis characterized by inflammation. Methods: We used a bioinformatics approach to compare the PGV-0 target with curcumin and diclofenac sodium as controls. We identified overlapping gene targets of bioactive compounds (PGV-0, curcumin, or diclofenac sodium) retrieved from the SwissTargetPrediction and GeneCards platforms, specifically for temporomandibular arthritis. An interaction model among targets was developed using the STRING database and Gene Ontology Panther to expound on the bioactive compound’s function on the key signaling pathway. Finally, we formulated a molecular docking prediction between the bioactive compound and the target protein marker derived from the previous analysis using Molecular Operating Environment tools. Results: This study found that curcumin and PGV-0 targeted different molecular pathways in temporomandibular arthritis compared to diclofenac sodium. Curcumin and PGV-0 shared a similar pathway to curcumin by modulating metalloproteinases (MMPs), especially MMP-9 and MMP-13. Moreover, diclofenac sodium influenced cyclooxygenase metabolism. Conclusion: In this study, PGV-0 targeted metalloproteinase in temporomandibular arthritis pathogenesis. This finding underlines the PGV-0 advantage in preventing metalloproteinase-related tissue damage in temporomandibular arthritis.
Analgesic, Anti-Inflammatory and Anti-Biofilm-Forming Activity of Potato (Solanum tuberosum L.) Peel Extract Wahyudi, Ivan Arie; Ramadhan, Fahri Reza; Wijaya, Rama Insan Kusuma; Ardhani, Retno; Utami, Trianna Wahyu
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp30-35

Abstract

The utilization of natural resources, one of which is plants, has been researched as an alternative to synthetic drugs because of their natural content. Potato (Solanum tuberosum L.) peels, the parts of potatoes that are often cut off and discarded, have been reported to have some phenolic compounds and flavonoids in their composition. The extract of potato peels was investigated for its analgesic, anti-inflammatory, and anti-biofilm-forming properties. A hot plate test was conducted to assess the analgesic activity in treatment doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg with paracetamol as the reference drug and distilled water as the negative control, while carrageenan-induced paw edema was used to assess anti-inflammatory activity in treatment doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg with diclofenac as the reference drug and distilled water as the negative control. Anti-biofilm-forming activity was tested by using the crystal violet assay. The results showed that, compared with the negative control, treatment doses of 100 mg/kg and 200 mg/kg significantly (p < 0.05) reduced pain stimuli, whereas a treatment dose of 100 mg/kg, 200 mg/kg, and 400 mg/kg significantly (p < 0.05) reduced the edema volume increment. However, compared with the positive control, paracetamol and diclofenac were associated with the least pain stimulus and the least edema volume increment, respectively. Potato peel extract against S. mutans biofilm formation demonstrated effectiveness (p < 0.05). Based on these data, it can be concluded that potato peel extract has analgesic, anti-inflammatory, and anti-biofilm-forming activities, as demonstrated in this study.
Co-Authors Anne Handrini Dewi Anne Handrini Dewi Aulia Rahman, Faaza Banun Kusumawardani Dian Kesumapramudya Nurputra Dwi Merry Christmarini Robin Dyah Listyarifah Edy Meiyanto Gloria Fortuna Hargo Utomo Ivan Arie Wahyudi Jansen, John Novitasari, Dhania Nunuk Purwanti Ramadhan, Fahri Reza Ryan Christian Pramuditya Trianna Wahyu Utami Wijaya, Rama Insan Kusuma