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All Journal Jurnal Biologi Tropis Jurnal Farmasi Medica (Pharmacy Medical Journal) PMJ Benzena Pharmaceutical Scientific Journal
Sa'ad, Muhammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Public Health

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A PENGUJIAN KADAR SENYAWA FLAVONOID EKSTRAK TERPURIFIKASI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS Sukmasnastiti, Messty; Suyono Saputri, Alip desi; Sa'ad, Muhammad
Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ) Vol. 7 No. 1 (2024): Volume 7, No 1, Tahun 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pmj.v7i1.48144

Abstract

Masyarakat Indonesi banyak yang mengonsumsi buah naga merah (Hylocereus polyrhizus) karena rasanya yang enak dan bermanfaat untuk kesehatan serta harganya yang ekonomis. Kulit buah naga merah (Hylocereus polyrhiz) memiliki banyak khasiat antara lain sebagai antioksidan dan imunomodulator. Salah satu kandungan senyawa sekunder yang terdapat pada kulit buah naga adalah flavonoid. Tujuan penelitian ini yaitu mempurifikasi ekstrak kental etanol 96% dan melakukan penetapan kadar. Metode yang digunakan dalam penelitian ini yaitu metode maserasi dengan etanol 96% yang dilakukan selama 5 hari, purifikasi ekstrak kental hasil dari maserasi dengan pelarut etil asetat. Kemuadian dilakukan analisis kuantitatif dengan spektrofotometri UV-Vis. Analisis kuantitatif penetapan kadar flavonoid total ekstrak terpurifikasi menggunakan spektrofotometri UV-Vis diperoleh panjang gelombang 430 nm dan Operating Time 27 menit dengan menggunakan baku kuersetin. Berdasarkan hasil penelitian penetapan kadar flavonoid total ekstrak terpurifikasi kulit buah naga merah (Hylocereus polyrhiz) yaitu sebesar 69,164 ± 0,0403 mgQE/g ekstrak (6,92 ± 0,01%). Kata kunci: Kulit buah naga merah, ekstrak purifikasi, spektrofotometri UV-Vis, kadar flavonoid
In Vivo Antidiabetic Potential Test of Suruhan’s (Peperomia pellucida) Purified Extract Using Alloxan-Induced Wistar Rats Sa'ad, Muhammad; Kusuma, Eka Wisnu; Susanti, Ira; Handayani, Dewi Uthamy
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7607

Abstract

Natural-based antidiabetic drugs need to be developed as an alternative to synthetic antidiabetic drugs to minimize side effects. Suruhan extract (Peperomia pellucida) is one of the natural ingredients that has antidiabetic activity. In vitro studies show that ethanol extract, hexane extract and purified ethanol extract have antidiabetic effectiveness. This study was conducted to test the antidiabetic activity of purified suruhan extract on alloxan-induced Wistar rats to confirm antidiabetic activity in vivo. A total of 8 test groups, each consisting of 3 rats induced by alloxan 125mg/KgBW and non-fasting blood glucose was checked at 0; 30; 60; 90; 120; and 150 minutes using a glucometer, then the percentage of decrease in blood glucose levels was calculated. Positive control using glibenclamide 0.45mg/KgBW, negative control using CMC-Na 0.5%. The treatment groups consisted of: Extract 20mg/KgBW (E20); Extract 40mg/KgBW (E40); Extract 80mg/KgBW (E80); Purified Extract 20mg/KgBW (P20); Purified Extract 40mg/KgBW (P40); and Purified Extract 80mg/KgBW (P80). Results showed the percentage decrease in blood glucose levels of E20; E40; E80; P20; P40; and P80 respectively: 39.93%; 42.29%; 46.93%; 38.34%; 55.34%; and 66.40%. The percentage decrease in blood glucose levels of the positive control group was 53.71%. The Purified Extract treatment groups of 40mg/KgBW and 80mg/KgBW showed the percentage decrease in blood glucose levels equivalent to and better than the positive control of glibenclamide 0.45mg/KgBW (p<0.05%). The purified extract was shown to have antidiabetic effects in vivo and is promising for use as an alternative antidiabetic drug.
PENETAPAN KADAR FLAVONOID REBUSAN DAN SEDUHAN DAUN TAPAK LIMAN (Elephantopus scaber) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Multi, Bryan Dwi; Saputri, Alip Desi Suyono; Sa'ad, Muhammad
BENZENA Pharmaceutical Scientific Journal Vol 3 No 01 (2024): BENZENA PHARMACEUTICAL SCIENTIFIC JOURNAL
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/benzena.v3i01.4425

Abstract

Latar bekalang :  Banyak penduduk Indonesia yang menggunakan tanaman obat atau jamu baik untuk menjaga kesehatan maupun untuk pengobatan karena sakit. Salah satu tanaman obat yang sering dikonsumsi oleh masyarakat yaitu tanaman tapak liman (Elephantopus scaber). Tanaman Tapak liman (Elephantopus scaber) diketahui mempunyai kandungan flavonoid yang cukup tinggi. Masyarakat Indonesia dalam mengkonsumsi tanaman obat menggunakan cara direbus dan diseduh.Tujuan:Tujuan dari penelitian ini adalah untuk mengetahui kadar flavonoid tertinggi pada dua metode ekstraksi (rebusan dan seduhan) menggunakan Spektrofotometri UV-Vis.Metode:Metode penelitian ini berupa metode eksperimental. Uji kualitatif dilakukan dengan uji KLT dan menghasilkan bercak dibandingkan dengan standar kuersetin. Uji kuantitatif dilakukan dengan penetapan kadar flavonoid total rebusan dan seduhan simplisia daun tapak liman dengan Spektrofotometri UV-Vis.Hasil: Hasil penetapan kadar flavonoid total didapatkan sebesar 2,448 ± 0,1597 mgQE/100ml dengan %KV 0,65 pada metode rebusan. Pada seduhan simplisia daun tapak liman kadar yang diperoleh sebesar 0,531 ± 0,0096 mgQE/100ml dengan %KV 1,81. Uji statistik menunjukkan nilai signifikansi dari perbedaan kadar flavonoid (p value 0,000 < 0,05).Kesimpulan: Kesimpulan pada penelitian ini terdapat perbedaan kadar flavonoid yang signifikan antara rebusan dan seduhan daun tapak liman. Metode rebusan memiliki kandungan flavonoid lebih besar dibanding metode seduhan.
POTENSI ANTIOKSIDAN REBUSAN DAN SEDUHAN TEMU MANGGA (Curcuma mangga) DENGAN METODE DPPH Halimah, Nur; Sa'ad, Muhammad
BENZENA Pharmaceutical Scientific Journal Vol 4 No 01 (2025): BENZENA PHARMACEUTICAL SCIENTIFIC JOURNAL
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/benzena.v4i01.6697

Abstract

Background : Free radicals are unstable molecules that cause oxidative stress and various chronic diseases. Antioxidant compounds can play a role in neutralizing free radicals. Plants as natural ingredients of traditional medicine contain secondary metabolite compounds that act as antioxidants. One of the plants used as an antioxidant is temu mangga (Curcuma mangga). The use of natural ingredients as medicine by the people in Indonesia mostly uses the boiling and steeping methods. Objective : This study aims to determine the antioxidant activity of boiled and brewed temu mangga rhizome (Curcuma mangga) compared to quercetin. And to determine the content of secondary metabolites that are responsible for antioxidant activity. Method : Phytochemical screening using color reagents to determine the content of secondary metabolites including: alkaloids, tannins, saponins, and flavonoids. Antioxidant activity was measured using the DPPH method using the parameter of the Inhibitory Concentration 50% (IC50) value with quercetin as a positive control. Result : The results showed that both methods have secondary metabolite compounds that are responsible for antioxidant activity, namely: alkaloids, tannins, saponins, and flavonoids. Both methods showed very strong antioxidant activity, with IC50 quercetin, boiling and brewing method respectively of 5.62 ppm; 5.75 ppm; and 5.53 ppm. The brewing method was slightly more effective, possibly because the temperature used was lower, thus maintaining the stability of thermolabile antioxidant compounds such as flavonoids. Conclusion : Extraction method has significant effect on antioxidant activity. The brewing method can be a more optimal traditional method to maintain antioxidant properties of Curcuma mangga.
Co-Authors Handayani, Dewi Uthamy Ira Susanti Kusuma, Eka Wisnu Multi, Bryan Dwi Nur Halimah Saputri, Alip Desi Suyono Sukmasnastiti, Messty Suyono Saputri, Alip desi