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All Journal Jurnal Biologi Tropis Jurnal Farmasi Medica (Pharmacy Medical Journal) PMJ
Sa'ad, Muhammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health

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A PENGUJIAN KADAR SENYAWA FLAVONOID EKSTRAK TERPURIFIKASI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS Sukmasnastiti, Messty; Suyono Saputri, Alip desi; Sa'ad, Muhammad
Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ) Vol. 7 No. 1 (2024): Volume 7, No 1, Tahun 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pmj.v7i1.48144

Abstract

Masyarakat Indonesi banyak yang mengonsumsi buah naga merah (Hylocereus polyrhizus) karena rasanya yang enak dan bermanfaat untuk kesehatan serta harganya yang ekonomis. Kulit buah naga merah (Hylocereus polyrhiz) memiliki banyak khasiat antara lain sebagai antioksidan dan imunomodulator. Salah satu kandungan senyawa sekunder yang terdapat pada kulit buah naga adalah flavonoid. Tujuan penelitian ini yaitu mempurifikasi ekstrak kental etanol 96% dan melakukan penetapan kadar. Metode yang digunakan dalam penelitian ini yaitu metode maserasi dengan etanol 96% yang dilakukan selama 5 hari, purifikasi ekstrak kental hasil dari maserasi dengan pelarut etil asetat. Kemuadian dilakukan analisis kuantitatif dengan spektrofotometri UV-Vis. Analisis kuantitatif penetapan kadar flavonoid total ekstrak terpurifikasi menggunakan spektrofotometri UV-Vis diperoleh panjang gelombang 430 nm dan Operating Time 27 menit dengan menggunakan baku kuersetin. Berdasarkan hasil penelitian penetapan kadar flavonoid total ekstrak terpurifikasi kulit buah naga merah (Hylocereus polyrhiz) yaitu sebesar 69,164 ± 0,0403 mgQE/g ekstrak (6,92 ± 0,01%). Kata kunci: Kulit buah naga merah, ekstrak purifikasi, spektrofotometri UV-Vis, kadar flavonoid
In Vivo Antidiabetic Potential Test of Suruhan’s (Peperomia pellucida) Purified Extract Using Alloxan-Induced Wistar Rats Sa'ad, Muhammad; Kusuma, Eka Wisnu; Susanti, Ira; Handayani, Dewi Uthamy
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7607

Abstract

Natural-based antidiabetic drugs need to be developed as an alternative to synthetic antidiabetic drugs to minimize side effects. Suruhan extract (Peperomia pellucida) is one of the natural ingredients that has antidiabetic activity. In vitro studies show that ethanol extract, hexane extract and purified ethanol extract have antidiabetic effectiveness. This study was conducted to test the antidiabetic activity of purified suruhan extract on alloxan-induced Wistar rats to confirm antidiabetic activity in vivo. A total of 8 test groups, each consisting of 3 rats induced by alloxan 125mg/KgBW and non-fasting blood glucose was checked at 0; 30; 60; 90; 120; and 150 minutes using a glucometer, then the percentage of decrease in blood glucose levels was calculated. Positive control using glibenclamide 0.45mg/KgBW, negative control using CMC-Na 0.5%. The treatment groups consisted of: Extract 20mg/KgBW (E20); Extract 40mg/KgBW (E40); Extract 80mg/KgBW (E80); Purified Extract 20mg/KgBW (P20); Purified Extract 40mg/KgBW (P40); and Purified Extract 80mg/KgBW (P80). Results showed the percentage decrease in blood glucose levels of E20; E40; E80; P20; P40; and P80 respectively: 39.93%; 42.29%; 46.93%; 38.34%; 55.34%; and 66.40%. The percentage decrease in blood glucose levels of the positive control group was 53.71%. The Purified Extract treatment groups of 40mg/KgBW and 80mg/KgBW showed the percentage decrease in blood glucose levels equivalent to and better than the positive control of glibenclamide 0.45mg/KgBW (p<0.05%). The purified extract was shown to have antidiabetic effects in vivo and is promising for use as an alternative antidiabetic drug.
Co-Authors Handayani, Dewi Uthamy Ira Susanti Kusuma, Eka Wisnu Sukmasnastiti, Messty Suyono Saputri, Alip desi