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All Journal E-Jurnal Medika Udayana Biology, Medicine, & Natural Product Chemistry
Santosa, Didi Nugroho
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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EFFECT OF DEHUMIDIFICATION ON Trigona spp. HONEY AS AN ANTIBACTERIAL AGAINST Streptococcus mutans Pratiwi, Deviyanti; Saristi, Salsabila Shafa; Santosa, Didi Nugroho; Rejeki, Putri
E-Jurnal Medika Udayana Vol 13 No 8 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i08.P02

Abstract

Honey comes from Trigona spp. has been known for its various health benefits and has the potential to be a natural ingredient that can be used as a preventive measure to inhibit and kill the main bacteria in dental caries (Streptococcus mutans). The dehumidification process using a humidifier is carried out to absorb honey water vapor so that the water content of the honey is reduced and the quality is better. The aim of this research is to determine the effect of dehumidification on Trigona spp. honey as an antibacterial for S.mutans. This research is a laboratory experiment with a post-test only control group design in vitro using the dilution method. Dehumidification is carried out on raw trigona honey to obtain dehumid trigona honey and honey water. As a control, 0.2% chlorhexidine and distilled water were used. The antibacterial activity test was carried out by calculating the total bacterial colonies using the total bacterial colony formula. The results of the study showed that the antibacterial activity of raw Trigona spp. honey had a significant difference against S.mutans with a total number of colonies of 0.4x106 CFU/mL compared to Trigona spp. dehum honey and honey water. Kruskal-Wallis analysis showed that there was a significant difference between the control group and the treatment group in inhibiting S.mutans. The conclusion is that raw Trigona spp. honey, Trigona spp. dehum honey, and honey water show differences in the antibacterial activity of S.mutans so that their use as a preventive measure in inhibiting S.mutans bacteria needs to be considered. Keywords : dehumidification, Trigona spp. honey, antibacterial, Streptococcus mutans
The Effect of Nano Chitosan Xylotrupes Gideon on Fibroblast Proliferation and Collagen Deposition in the Oral Mucosa of Rattus norvegicus Komariah, Komariah; Roeslan, Muhammad Orliando; Anggraeni, Rezky; Santosa, Didi Nugroho; Rennata, Aubrey Kanya
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.23-30

Abstract

Lacerations of the oral mucosa are a common clinical problem and require an efficient healing process. The wound healing process involves the haemostasis, inflammation, proliferation, and maturation phases. During the proliferation phase, fibroblasts play an important role in migration and proliferation to produce collagen, which connects the wound's edges and provides strength and stability to the scar tissue that forms. Chitosan from the horned beetle (Xylotruphes gideon) contains active compounds that are thought to accelerate the healing process by increasing the activity of inflammatory cells and fibroblast proliferation. Physical modification into nanoparticles facilitates penetration into the mucosal layer, thereby accelerating collagen production and re-epithelialization. This study aimed to determine the effect of X. gideon nanokitosan administration on increasing fibroblast proliferation and collagen formation in vivo. The study was divided into five groups: positive control, negative control, and nano chitosan treatment at 3000, 1500, and 750µg/mL. All groups of experimental animals were previously given an injury to the right cheek mucosa with a No. 12 scalpel blade, and nano chitosan was administered topically. The increase in fibroblasts and collagen deposits was determined by HE and Masson's Trichrome staining on days 3, 7, and 14, which were observed microscopically and using ImageJ. The results showed a significant increase in fibroblasts on day 3 and collagen fibre deposition on day 14 in all treatment groups. The group with 750 ppm nano chitosan showed the best results in accelerating wound healing regarding fibroblast proliferation and collagen deposition. Conclusion: Nano chitosan X. gideon at a concentration of 750 ppm effectively accelerates wound healing in the cheek mucosa of mice by increasing fibroblast proliferation and collagen deposition.
Co-Authors Anggraeni, Rezky Deviyanti Pratiwi Komariah Komariah Putri Rejeki Rennata, Aubrey Kanya Roeslan, Muhammad Orliando Saristi, Salsabila Shafa