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All Journal Jambura Journal of Chemistry KNOWLEDGE: Jurnal Inovasi Hasil Penelitian dan Pengembangan
Tukan, Maria Magdalena Nona Motu
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Arts Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Physics Other

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Antioxidant Activity and Inhibition of Α-Glucosidase from Yellow Root Extract (Fatuoa Pilosa Gaudich) In Vitro Tukan, Maria Magdalena Nona Motu; Falah, Samsul; Andrianto, Dimas; Najmah, Najmah
Jambura Journal of Chemistry Vol 5, No 2 (2023): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v5i2.20503

Abstract

Yellow root (Fatoua pilosa Gaudich) is a type of plant which is found on lowland mostly in Japan, China and usually grow in the dry bushes. Traditionally, the yellow root (Fatuoa pilosa Gaudich) is used to treat the disease of diabetes mellitus and as antiinflamation. One of the causes of diabetes disease is oxidative stress. Oxidative stress can be reduced by using antioxidants. This research aims to explore antioxidant activity and inhibition of α-glucosidase enzyme from yellow root extract (Fatoua pilosa Gaudich.). Quantitative determination of total phenolic by using folin-ciocalteu method declared as gallic acid equivalents (GAE) per gram of extract, levels of total flavonoids with AlCl3 method declared as Quercetin equivalen (QE), and antioxidant activity in fertilization with the DPPH (2, 2-diphenyl-1- picrylhydrazyl) stated in the IC50 (concentration inhibition). The Results showed that the highest levels of total flavonoids was in extract ethanol 96% 199.44 g QE/g extract, while the total phenolic levels highest in 70% ethanol extracts 211.54 mg GAE/g extract. The highest IC50 obtained from antioxidant test of yellow root extrat was 70% ethanol extract 14.62 μg/mL and the most active fraction that can inhibit α-glucosidase enzyme was of ethyl acetate fraction with IC50 values 680.54 μg/mL.
AKTIVITAS ANTIOKSIDAN PADA TINTA GURITA (Octopoda sp) DI KABUPATEN FLORES TIMUR Prada, Maria Noralista; Tukan, Maria Magdalena Nona Motu; Peni, Donata
KNOWLEDGE: Jurnal Inovasi Hasil Penelitian dan Pengembangan Vol. 5 No. 3 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia (P4I)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/knowledge.v5i3.7010

Abstract

Octopuses are mollusks that live on coral reefs. Octopuses (Octopoda sp.) have a unique characteristic: an ink sac that produces a thick, black liquid that serves as a defense and resistance against enemy attack. This study aims to determine the antioxidant activity of octopus ink in East Flores Regency. The implementation of this research began with taking octopus ink samples at PT. Biru Muda Perkasa then prepared in the Fisheries Product Technology processing room of the Larantuka Teacher Training and Technology Institute and extracted using 70% Ethanol solvent. The extraction results were then tested for antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method which was expressed as an IC50 value (the concentration at which the sample can inhibit DPPH by 50%). The results showed that the antioxidant activity obtained in the 70% ethanol extract sample was 435.8 ppm, this result was obtained through calculations using a linear regression equation, namely y = 0.1031x + 5.0682. The results above indicate that the activity of the octopus ink ethanol extract is classified as weak activity. ABSTRAKGurita merupakan hewan mollusca yang habitatnya pada terumbu karang. Gurita (Octopoda sp) memiliki karakteristik yang unik yaitu adanya kantung tinta yang menghasilkan tinta pekat berupa cairan berwana hitam  yang berfungsi sebagai benteng pertahanan dan perlawanan ketika diserang musuh. Penelitian ini bertujuan untuk  untuk mengetahui aktivitas antioksidan pada tinta gurita di Kabupaten Flores Timur. Pelaksanaan penelitian ini diawali dengan pengambilan sampel tinta gurita di PT. Biru Muda Perkasa kemudian di preparasi diruang pengolahan Teknologi Hasi Perikanan Institut Keguruan dan Teknologi Larantuka dan diekstraksi menggunakan pelarut Etanol 70%. Hasil ekstrasi selanjurnya diuji aktivitas antioksidan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang dinyatakan dengan nilai IC50 (konsentrasi dimana sampel dapat menghambat DPPH sebanyak 50%). Hasil penelitian menunjukan bahwa aktivitas antioksidan yang diperoleh pada sampel ekstrak etanol 70% adalah 435,8 ppm, hasil ini di peroleh melalui perhitungan yang menggunakan persamaan regresi linear yaitu y = 0,1031x + 5,0682. Hasil diatas menunjukan aktivitas ektrak etanol tinta gurita tergolong dalam aktivitas lemah.
Co-Authors DIMAS ANDRIANTO Falah, Samsul Najmah, Najmah Peni, Donata Prada, Maria Noralista