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Uji Aktivitas Enzim Selulase Baglog Jamur Pleurotus cystidiosus, Pleurotus ostreatus dan Auricularia auricula dengan Pelarut Berbeda Zulfa, Astri; Qonitah, Tsabitah Athifah; Ragamustari, Safendrri K; Prasasty, Vivitri Dewi
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Cellulase enzyme activity is essential for the breakdown of cellulose, a major component of plant cell walls. This enzyme is produced by a variety of microorganisms, including fungi such as mushrooms. This study aimed to evaluate the cellulase enzyme activity extracted from the baglogs of three different mushrooms: Pleurotus cystidiosus (brown oyster mushroom), Pleurotus ostreatus (white oyster mushroom), and Auricularia auricula (wood ear mushroom). The extraction was conducted using three different solvents: aquadest (distilled water), saline solution, and citrate buffer at pH 4. The cellulase activity was assessed under optimal conditions of 60 °C incubation temperature and 60 minutes incubation time. The results indicated that the lowest cellulase activity was found in the baglogs of Pleurotus cystidiosus extracted with aquadest, measuring at 0.0253 IU/mL. Conversely, the highest cellulase activity was observed in the baglogs of Pleurotus ostreatus extracted with aquadest, with an activity level of 0.0728 IU/mL. The glucose concentration, which serves as an indicator of the enzymatic hydrolysis of carboxymethyl cellulose (CMC), was highest in Pleurotus ostreatus extracted with aquadest at 393.043 ppm. These findings suggest that Pleurotus ostreatus is the most effective species for cellulase production among the tested mushrooms when extracted with aquadest. The optimal extraction and assay conditions determined in this study can guide future research and industrial applications in enhancing cellulase enzyme production from mushroom baglogs, particularly in bioconversion processes and bioethanol production.
Water Quality Index Analysis of Situ Cilodong, Depok, West Java Using Storet Method Zulfa, Astri; Apriansyah, Farhan; Qonitah, Tsabitah Athifah; Vansha, Fahriza Kemal; Utami, Marcelita Putri; Bakri, Hendrawan; Hoiroh, Ziyadatul; Kamila, Afifah Intan
Journal of Tropical Biodiversity Vol 4 No 1 (2023): December 2023
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v4i1.205

Abstract

Situ Cilodong is located in Kalibaru, Cilodong, Depok. Situ Cilodong served as a beautiful, strategic area and thus served as a local water tourism. However, the presence of a human activity causes it to contain household wastes and other fillings that leak into the body of the water. The study was intended to determine the quality of the water and was held by storet methods. Storets method work by comparing data on water quality with water quality adjusted to it in order to determine water quality status. The parameters used in this study are the physical parameters in the form of temperature and brightness, also chemical in the form of pH and DO, all parameters was measure insitu in the morning. We also do identyfication of plankton. Data retrieval times occurred periodically each week of November 2021. Based on its designation, Situ Cilodong is included in class 2. The sampling station point being used is at the inlet station, center and outlet. The result of research is that the waters at Situ Cilodong was classified as a light polluted lake based on stroret's methods score on the inlet -10 station, -8 and -10 outlets. The most frequently identified type of planton species is from the phytoplanton group.
Phytochemistry and Potential of Sargassum binderi, Sargassum cinereum, Padina australis, and Turbinaria conoides as Medicinal Ingredients Priamanatha , Dandy; Handayani, Sri; Zulfa, Astri
Journal of Tropical Biodiversity Vol 5 No 3 (2025): August 2025
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v5i3.294

Abstract

This study investigated the phytochemicals and potential from macroalgae Sargassum binderi, Sargassum cinereum, Padina australis and Turbinaria conoides as material medicine. Samples in the study are taken from the sea around Kepulauan Seribu. Qualitative and quantitative tests conducted in the laboratory Nasional University Chemistry and Research Center Laboratory Plant Spices and Medicines. Research focuses on composition of phytochemicals consisting of groups of alkaloids, tannins, flavonoids, and saponins, then see its potential as a material drug through scientific studies. The results found in the research on every sample found contain all group phytochemicals in a way qualitative. Apart from that, levels of phytochemicals obtained are different. Sargassum binderies own high levels of flavonoids and saponins so that potential become drug as antioxidant, antiatherosclerotic, anti-inflammatory, antithrombogenic, antitumor, antiosteoporotic , antiviral, antibacterial, antifungal, and antiparasitic. While level tannin highest owned by Turbinaria conoides so that potential become material drug as antioxidant, antibacterial, antiviral, anticarcinogenic, anti-inflammatory, antiallergic, and vasodilator agents. Padina australis and Sargassum cinereum in a way sequentially own level highest from flavonoid and saponin groups, so that potential become material drug as antioxidant, antiatherosclerotic, anti-inflammatory, antithrombogenic, antitumor, antiosteoporotic, antiviral, antibacterial, antifungal, antiparasitic, and has hemolytic. All samples in a way qualitative contain alkaloids, so that has the potential to also become a material drug as anticancer, antibacterial, antiviral, antifungal, cytotoxic to herbivorous, analgesic, and relaxant muscle. Research more carry on about activity pharmacology, compounds phytochemicals specific, alkaloid levels, and dosage best can open knowledge about material drug from more macroalgae complete