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All Journal Pro-Life Journal of Tropical Biodiversity
Qonitah, Tsabitah Athifah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Immunology & microbiology

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Uji Aktivitas Enzim Selulase Baglog Jamur Pleurotus cystidiosus, Pleurotus ostreatus dan Auricularia auricula dengan Pelarut Berbeda Zulfa, Astri; Qonitah, Tsabitah Athifah; Ragamustari, Safendrri K; Prasasty, Vivitri Dewi
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Cellulase enzyme activity is essential for the breakdown of cellulose, a major component of plant cell walls. This enzyme is produced by a variety of microorganisms, including fungi such as mushrooms. This study aimed to evaluate the cellulase enzyme activity extracted from the baglogs of three different mushrooms: Pleurotus cystidiosus (brown oyster mushroom), Pleurotus ostreatus (white oyster mushroom), and Auricularia auricula (wood ear mushroom). The extraction was conducted using three different solvents: aquadest (distilled water), saline solution, and citrate buffer at pH 4. The cellulase activity was assessed under optimal conditions of 60 °C incubation temperature and 60 minutes incubation time. The results indicated that the lowest cellulase activity was found in the baglogs of Pleurotus cystidiosus extracted with aquadest, measuring at 0.0253 IU/mL. Conversely, the highest cellulase activity was observed in the baglogs of Pleurotus ostreatus extracted with aquadest, with an activity level of 0.0728 IU/mL. The glucose concentration, which serves as an indicator of the enzymatic hydrolysis of carboxymethyl cellulose (CMC), was highest in Pleurotus ostreatus extracted with aquadest at 393.043 ppm. These findings suggest that Pleurotus ostreatus is the most effective species for cellulase production among the tested mushrooms when extracted with aquadest. The optimal extraction and assay conditions determined in this study can guide future research and industrial applications in enhancing cellulase enzyme production from mushroom baglogs, particularly in bioconversion processes and bioethanol production.
Edible Mushrooms in The Surroundings of Bangbayang Village, Situraja, Sumedang, West Java Qonitah, Tsabitah Athifah; Aryyanto, Muhammad Raul Alfadri; Suteja, Hardiansyah; Murti, Alena Puspa; Noverita, Noverita
Journal of Tropical Biodiversity Vol 4 No 1 (2023): December 2023
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v4i1.203

Abstract

Bangbayang is one of the villages located in the Situraja Subdistrict, Sumedang Regency, West Java. The natural conditions around the area are very cool with various types of plants, which also make it likely to find mushrooms, especially macro mushrooms that have the potential as food ingredients, but have not been reported. This study aims to inventory macro mushrooms with the potential as food ingredients in Bangbayang Village, Situraja Subdistrict, Sumedang Regency, West Java. The research method used is exploratory, and each sample of macro mushrooms obtained is observed based on the macroscopic characteristics of its fruiting body. The results of the study obtained a total of 12 species of macro mushrooms with the potential as food ingredients, namely; Agaricus campestris, Auricularia auricula, A. cornea, Boletus edulis, Cortinarius caperatus, Filoboletus manipularis, Lentinus sajor-caju, Lepista nuda, Russula rosea, Sarcomyxa edulis, Termitomyces clypeatus, and Tricholoma fraticum.
Water Quality Index Analysis of Situ Cilodong, Depok, West Java Using Storet Method Zulfa, Astri; Apriansyah, Farhan; Qonitah, Tsabitah Athifah; Vansha, Fahriza Kemal; Utami, Marcelita Putri; Bakri, Hendrawan; Hoiroh, Ziyadatul; Kamila, Afifah Intan
Journal of Tropical Biodiversity Vol 4 No 1 (2023): December 2023
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v4i1.205

Abstract

Situ Cilodong is located in Kalibaru, Cilodong, Depok. Situ Cilodong served as a beautiful, strategic area and thus served as a local water tourism. However, the presence of a human activity causes it to contain household wastes and other fillings that leak into the body of the water. The study was intended to determine the quality of the water and was held by storet methods. Storets method work by comparing data on water quality with water quality adjusted to it in order to determine water quality status. The parameters used in this study are the physical parameters in the form of temperature and brightness, also chemical in the form of pH and DO, all parameters was measure insitu in the morning. We also do identyfication of plankton. Data retrieval times occurred periodically each week of November 2021. Based on its designation, Situ Cilodong is included in class 2. The sampling station point being used is at the inlet station, center and outlet. The result of research is that the waters at Situ Cilodong was classified as a light polluted lake based on stroret's methods score on the inlet -10 station, -8 and -10 outlets. The most frequently identified type of planton species is from the phytoplanton group.
Co-Authors Apriansyah, Farhan Aryyanto, Muhammad Raul Alfadri Bakri, Hendrawan Hoiroh, Ziyadatul Kamila, Afifah Intan Murti, Alena Puspa Noverita Ragamustari, Safendrri K Suteja, Hardiansyah Utami, Marcelita Putri Vansha, Fahriza Kemal Vivitri Dewi Prasasty Zulfa, Astri