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Immunophenotyping vs. Bone Marrow Aspiration in Pediatric Acute Leukemia: A Comparative Analysis Satria Utomo; Yulia, Dwi; Elfira Yusri; Rismawati Yaswir; Husni; Desywar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1289

Background: Acute leukemia is a significant global health concern, with increasing prevalence worldwide and in Indonesia. Accurate diagnosis and classification of acute leukemia subtypes, primarily acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML), are crucial for effective treatment. Immunophenotyping and bone marrow aspiration (BMA) are commonly used diagnostic methods, each with its strengths and limitations. This study aimed to analyze the concordance between immunophenotyping and BMA in diagnosing and classifying acute leukemia in children. Methods: A retrospective cross-sectional study was conducted on 46 children diagnosed with acute leukemia at Dr. M. Djamil General Hospital Padang from January 2022 to July 2023. Data were collected from medical records, including patient demographics, immunophenotyping results, and BMA findings. Concordance between the two diagnostic methods was analyzed using Fisher's exact test. Results: The study population consisted of 30 (65.2%) males and 16 (34.8%) females, with a median age of 4 years. Immunophenotyping identified 24 (52.2%) cases as ALL and 22 (47.8%) as AML. BMA classified 26 (56.5%) cases as ALL and 20 (43.5%) as AML. There was a high concordance between the two methods, with only 2 (4.3%) cases showing discordant results. These two cases were classified as AML by immunophenotyping but as ALL by BMA. Conclusion: Immunophenotyping and BMA demonstrate a high level of concordance in diagnosing and classifying acute leukemia in children. The few discordant cases highlight the importance of considering both methods, especially in challenging cases, to ensure accurate diagnosis and appropriate treatment.

Differences in Cerebrospinal Fluid Glucose Levels in Meningitis Patients Based on Examination Time: A Single Center Observational Study at Dr. M. Djamil General Hospital, Padang, Indonesia Haryando, Herpika Septi; Elfira Yusri; Desywar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 9 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i9.1063

Background: Cerebrospinal fluid (CSF) is a vital fluid in the central nervous system that can provide important diagnostic information, especially in cases of meningitis. CSF glucose is one of the key parameters that can help in the diagnosis and monitoring of the patient's condition. However, the stability of glucose in the CSF after sampling is of concern due to the potential for levels to decrease over time. Methods: This cross-sectional analytical observational study was conducted at Dr. M. Djamil General Hospital Padang between May and August 2023. CSF samples from patients with suspected meningitis were checked for glucose levels at three time points: the first 1 hour, 2 hours, and 4 hours after sample collection. Patients with puncture trauma and late delivery of samples (more than 30 minutes) were excluded. Results: This study involved [number of patients] CSF samples that met the inclusion criteria. The mean decrease in CSF glucose levels between the 1-hour and 2-hour examination was 5.667 mg/dL (±0.75 mg/dL), while between 1 hour and 4 hours, it was 12.183 mg/dL (±1.549 mg/dL). Statistical analysis showed significant differences (p < 0.001) between the three examination time points. Conclusion: Glucose levels in the CSF of meningitis patients decreased significantly over time after sampling. This emphasizes the importance of checking CSF glucose as soon as possible to obtain accurate results and avoid misinterpretation in the diagnosis and treatment of meningitis.

Differences in D-Dimer Levels in Acute Ischemic and Hemorrhagic Stroke: Observational Study in the Emergency Department of Dr. M. Djamil General Hospital, Padang, Indonesia Yunus, Dyniyah; Elfira Yusri; Desywar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 8 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i8.1056

Background: Stroke is the second largest cause of death worldwide with a high morbidity rate of up to 50% of survivors get chronic disability. Rapid diagnosis in patients with suspected acute ischemic or hemorrhagic stroke is very important to determine management and prognosis. D-dimer is an indirect marker of fibrinolysis which functions as a significant marker of activation of coagulation and fibrinolysis. This study aims to determine the differences in D-dimer levels in ischemic and acute hemorrhagic stroke patients in the emergency room (ER) of Dr. M. Djamil General Hospital Padang. Methods: Analytical observational research by cross-sectional design was carried out on 56 samples consisting of 28 acute ischemic and 28 acute hemorrhagic stroke samples for the period December 2022-June 2023. D-dimer levels and CTscan checked on each group and analysis is carried out. Results: The most common characteristics of research subjects were men, namely 35 patients (62.5%) and aged 51-60 years (32.1%). The mean D-dimer levels for ischemic and hemorrhagic strokes were 794.33 ng/mL (±2.63) and 1288.25 ng/mL (±2.51) with a p-value = 0.055. Conclusion: The mean D-dimer in acute hemorrhagic stroke was higher than in acute ischemic stroke but there was no statistically significant difference. The D-dimer examination cannot differentiate the type of stroke that occurred.

Differences in Cerebrospinal Fluid Glucose Levels in Meningitis Patients Based on Examination Time: A Single Center Observational Study at Dr. M. Djamil General Hospital, Padang, Indonesia Haryando, Herpika Septi; Elfira Yusri; Desywar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 9 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i9.1063

Background: Cerebrospinal fluid (CSF) is a vital fluid in the central nervous system that can provide important diagnostic information, especially in cases of meningitis. CSF glucose is one of the key parameters that can help in the diagnosis and monitoring of the patient's condition. However, the stability of glucose in the CSF after sampling is of concern due to the potential for levels to decrease over time. Methods: This cross-sectional analytical observational study was conducted at Dr. M. Djamil General Hospital Padang between May and August 2023. CSF samples from patients with suspected meningitis were checked for glucose levels at three time points: the first 1 hour, 2 hours, and 4 hours after sample collection. Patients with puncture trauma and late delivery of samples (more than 30 minutes) were excluded. Results: This study involved [number of patients] CSF samples that met the inclusion criteria. The mean decrease in CSF glucose levels between the 1-hour and 2-hour examination was 5.667 mg/dL (±0.75 mg/dL), while between 1 hour and 4 hours, it was 12.183 mg/dL (±1.549 mg/dL). Statistical analysis showed significant differences (p < 0.001) between the three examination time points. Conclusion: Glucose levels in the CSF of meningitis patients decreased significantly over time after sampling. This emphasizes the importance of checking CSF glucose as soon as possible to obtain accurate results and avoid misinterpretation in the diagnosis and treatment of meningitis.

The Role of Ferritin Levels on Vitamin D Status in Pregnant Women: An Observational Single Center Study at Hermina Hospital, Padang, Indonesia Hidayat, Hedo; Efrida; Elfira Yusri
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 9 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i9.1070

Background: Vitamin D deficiency during pregnancy is associated with poor pregnancy outcomes. Research shows a complex interaction between iron and vitamin D. Pregnant women are susceptible to iron deficiency due to increased iron requirements during pregnancy. Ferritin reflects body iron stores and may decrease before serum iron. This study aims to analyze the relationship between ferritin levels and vitamin D status in pregnant women. Methods: This retrospective comparative cross-sectional analytical observational study involved pregnant women in the 1st, 2nd, and 3rd trimesters who underwent antenatal care at Hermina Hospital Padang from February to August 2023. Vitamin D (25(OH)D3) and ferritin were measured using enzyme-linked fluorescence assay (ELFA). Univariate and bivariate analysis used the Chi-square and odds ratio (OR) tests, with a significance of p<0.05. Results: Of 163 pregnant women (mean age 30.4 years), median ferritin levels were 25.85 ng/mL and 25(OH)D3 15.5 ng/mL. Low ferritin was found in 63.80% of subjects and sufficient vitamin D in 31.90%, insufficiency in 33.74%, and deficiency in 34.36%. There was no correlation between low ferritin and vitamin D insufficiency (OR=2.04; 95% CI 0.94-4.42; p=0.700). However, there was a significant correlation between low ferritin and vitamin D deficiency (OR=6.59; 95% CI 2.68-16.18; p=0.000). Conclusion: Pregnant women with low ferritin have a 6.59 times higher risk of experiencing vitamin D deficiency.

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