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DNA Barcoding of Brevibora cheeya from Bumang River, Bangka Island Lestari, Eva; Almagribi, Siti PNIK; Lindiatika, Lindiatika; Khanati, Olivia; Donalista, Donalista; Anjani, Tiara P; Kurniawan, Andri; Syarif, Ahmad F; Kurniawan, Ardiansyah
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 13 No. 1 (2024): JLSO
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jlso.13.1.1024.671

Abstract

Brevibora cheeya is a cyprinid fish found in Bangka, Belitung, Sumatra, and Kalimantan. Bangka locals call this fish Seluang. This species is often considered Brevibora dorsiocellata due to its similar morphology. Molecular methods that have never been used for this species on Bangka Island can be used to confirm this species. This study uses COI genes (DNA barcoding) to molecularly identify Brevibora cheeya fish from Bangka Island and analyze their relationships. The sampling process was conducted in Bumang River, Bangka Island, in February 2023, and identification was conducted at the University of Bangka Belitung Biological Laboratory. NCBI BLAST and MEGA 11 were used for data analysis. The results showed that fish samples from Bangka Island have intraspecific genetic relatedness to B. cheeya from Belitung Island, Bangka Island, Central Kalimantan, and South Sumatra, with genetic distances ranging from 0% to 0.9%. The closest genetic relationship to Rasbora dorsiocellata is 3.2%. The phylogenetic tree showed a bootstrap value of 91 for relationships with the Bangka, Belitung, and Central Kalimantan databases and a bootstrap value of 95 for relationships with the Bangka and South Sumatra databases.
RESPON IKAN NILA (OREOCHROMIS NILOTICUS) DAN IKAN LELE (CLARIAS GARIEPINUS) YANG DIINFEKSI AEROMONAS HYDROPHILLA DENGAN EMPAT TINGKAT PASASE ANGGI, ANGGI; PASARIBU, TANIA A; HUTABARAT, NAOMI; DAMAYANTI, SILVI M; KRISTIN, ESTER P; FAKHRI, MUHAMMAD; ANJANI, TIARA P; KURNIAWAN, ARDIANSYAH
GANEC SWARA Vol 17, No 4 (2023): Desember 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i4.637

Abstract

Tilapia and catfish are some of the freshwater fisheries commodities that occupy the top rank in terms of the amount of production produced. Disease attacks in Tilapia and Catfish cultivation such as Motile Aeromonas Septicemia (MAS) can be detrimental to fish farmers. Motile Aeromonas Septicemia (MAS) disease is caused by the bacteria Aeromonas hydrophilla. This research aims to determine the differences in the physical condition of Tilapia and Catfish which were injected with Aeromonas hydrophilla bacteria and had been treated using the Koch Postulate method four times to reach 50% fish mortality. In this study, the Tilapia and Catfish samples used were 6-8 cm in size with a bacterial infection dose of 0.1ml/fish. Tilapia showed clinical symptoms of thin fins and the injection site began to become inflamed, while catfish experienced bleeding and wounds. Catfish tend to experience more serious injuries after exposure to A. hydrophilla compared to Tilapia due to differences in response to disease symptoms. Tilapia fish show symptoms of loose scales due to bacterial infections such as Aeromonas dhakensis, Pseudomonas mosselii, and Microbacterium paraoxydans.