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The Potential Use of Red Ginger (Zingiber officinale Roscoe) Dregs as Analgesic Febriani, Yessi; Riasari, Hesti; Winingsih, Wiwin; Aulifa, Diah Lia; Permatasari, Ayu
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Red ginger (Zingiber officinale Roscoe) had been used as excipient in jamu for most people in Indo- nesia. Based on recent research, ethanol extract of red ginger had analgesics activity. The purpose of this research to determine analgesic activity content in water extract of the fresh and dry red ginger and ethanol extract of fresh and dry of red ginger dregs. Fresh and dry of red ginger extracted using decoc- tion method, and the dregs of fresh and dry red ginger extracted using soxhletation method in ethanol 96% at 400C. Red ginger water extract and red ginger dregs extract had been examined for analgesic by witkin method. The results showed, fresh and dry red ginger water extract had analgesic activity for 25 minutes but the activity reduced in 30 minutes. Fresh and dry red ginger dregs ethanol extract still had analgesic activity until 30 minutes. TLC chromatogram was sprayed with FeCl3 and formed two spots with grey colour for both extracts. After sprayed with vanillin sulfate, it formed violet and blue spots from both extracts. From the retention factor (Rf) , possibility shogaol and gingerol contained in extract that have analgesic activity. Key words: Analgesic, dregs, phenolic, red ginger (Zingiber o cinale Roscoe), Witkin method.

PEMBUATAN DAN KARAKTERISASI NATRIUM KURKUMIN Winingsih, Wiwin; Husein, Sri Gustini; Sundalian, Melvia
JURNAL FARMASI GALENIKA Vol 5 No 1 (2018): Jurnal Farmasi Galenika Volume 5 No. 1, 2018
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.604 KB)

Kunyit (Curcuma domestica L.) termasuk family Zingiberaceae yang banyak terdapat di daerah tropis dan subtropis termasuk Indonesia. Kunyit telah banyak digunakan sebagai pewarna makanan, penambah rasa, dan obat tradisional. Peningkatan kelarutan kurkumin telah terbukti dapat meningkatkan bioavaibilitas dan juga efek farmakologinya. Pembuatan garam kurkumin larut air dilakukan dengan cara reaksi penggaraman dengan menggunakan natrium metoksida sehingga menghasilkan natrium kurkumin yang diharapkan dapat meningkat kelarutannya. Selain itu pada penelitian ini juga dilakukan karakterisasi garam kurkumin dengan spektrofotometri UV-Vis, FTIR, LC-MS, HPLC, dan KLT. Garam natrium Kurkumin dengan perbandingan molnatrium : kurkumin ( 1:1 ) dapat meningkatkan kelarutan kurkumin dalam air sebesar 620 kali. Hasil karakteristik garam kurkumindengan spektrofotometri menunjukann adanya perubahan  panjang gelombang maksimum (λ maks) dari 450 nm menjadi 350 nm. Garam natrium kurkumin menunjukkan adanya substitusi atom H oleh natrium yang mengakibatkan turunnya intensitas puncak pada daerah 3500-3000 cm-1. Hasil ujikarakteristik dengan KLT garam kurkumin dan kurkumin memiliki sedikit perbedaan nilai Rf. Garam natrium kurkumin dapat dibuat dengan mereaksikan natrium metoksida dan kurkumin.

The Potential Use of Red Ginger (Zingiber officinale Roscoe) Dregs as Analgesic Yessi Febriani; Hesti Riasari; Wiwin Winingsih; Diah Lia Aulifa; Ayu Permatasari
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Red ginger (Zingiber officinale Roscoe) had been used as excipient in jamu for most people in Indo- nesia. Based on recent research, ethanol extract of red ginger had analgesics activity. The purpose of this research to determine analgesic activity content in water extract of the fresh and dry red ginger and ethanol extract of fresh and dry of red ginger dregs. Fresh and dry of red ginger extracted using decoc- tion method, and the dregs of fresh and dry red ginger extracted using soxhletation method in ethanol 96% at 400C. Red ginger water extract and red ginger dregs extract had been examined for analgesic by witkin method. The results showed, fresh and dry red ginger water extract had analgesic activity for 25 minutes but the activity reduced in 30 minutes. Fresh and dry red ginger dregs ethanol extract still had analgesic activity until 30 minutes. TLC chromatogram was sprayed with FeCl3 and formed two spots with grey colour for both extracts. After sprayed with vanillin sulfate, it formed violet and blue spots from both extracts. From the retention factor (Rf) , possibility shogaol and gingerol contained in extract that have analgesic activity. Key words: Analgesic, dregs, phenolic, red ginger (Zingiber o cinale Roscoe), Witkin method.

Pengaruh Tempat Tumbuh Terhadap Kadar Alfa Tokoferol Pada Buah Mengkudu (Morinda Citrifolia L.): The Effect of Location on Alfa Tocopherol Levels in Noni Fruit (Morinda Citrifolia L.) Sri Gustini Husein; Wiwin Winingsih; Nurdianti Nurdianti; Achmad Zainuddin
Proceeding of Mulawarman Pharmaceuticals Conferences Vol. 13 (2021): Proc. Mul. Pharm. Conf.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Alpha tocopherol (?-tocopherol) contained in noni fruit (Morinda citrifolia L.) is one of the antioxidants that contribute to pharmacological effects. The noni plant is a tropical plant that has broad growing requirements, so that this plant can grow anywhere. Although easy to find, the environmental conditions where noni grows will have an influence on the amount of ?-tocopherol it contains, one of which is the difference in where it grows. The purpose of this study was to determine the levels of ?-tocopherol in the n-hexane fraction of noni fruit, which grows in three different growing places. Analysis for ?-tocopherol by High Performance Liquid Chromatography (HPLC) with a validated analytical method. The results of system suitability test HPLC System for ?- tocopherol analysis were as follows isocratic elution system of a mobile phase 100% methanol, uv detector at a wavelength of 292 nm using the column C18 (150 ? 4, 6mm, 5?m) flow rate of 1 ml / min. The method had linear concentration range from 1-25 ppm, with R2 0.9967. The accuracy of this method that represented by recovery was 98.02-99.252%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 0.262% and has good specificity. From the analysis, it was found that the content of ?-tocopherol in noni fruit was different and had a positive correlation with the height where it grew, namely in Pameungpeuk (18 m asl) of 0.0316%; Kiaracondong (173 m asl) of 0.0637%; and Lembang (1200 m asl) at 1.0853%.

PENINGKATAN KELARUTAN EKSTRAK LADA (Piper nigrum L.) DALAM AIR DAN KARAKTERISASINYA Alaris Darasito Damanik; Ricky Johanes Hutagaol; Fitriyani Fitriyani; Adang Firmansyah; Wiwin Winingsih
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 9, No 1 (2020)
Publisher : Sekolah Tinggi Farmasi Indonesia

Ekstrak lada mengandung banyak senyawa kimia yang memiliki aktivitas farmakologi, salahsatu senyawa dominannya yaitu piperin. Pemanfaatan ekstrak lada sebagai obat terkendalamasalah kelarutannya yang rendah dalam air. Penelitian ini bertujuan untuk meningkatkankelarutan ekstrak lada dalam air dengan metode penggaraman menggunakan NaOCH3 atauNaOH dengan variasi perbandingan mol. Hasil sintesis diuji kelarutan dengan spektrofotometerUV-Vis dan gravimetri, uji stabilitas terhadap asam dan basa dan lama penyimpanan sertakarakterisasi dengan KLT, Spektrofotometer UV-Vis, dan FTIR. Ekstrak lada yang disintesismenggunakan NaOCH3 memiliki rendemen yang lebih baik daripada menggunakan NaOH yaitu14,8 %. Hasil sintesis dengan NaOCH3 dengan perbandingan mol 2:1 memiliki rendemen yangpaling baik yaitu 19,15%. Ekstrak lada yang disintesis dengan NaOCH3 dengan perbandinganmol 2:1 mengalami peningkatan kelarutan sebanyak 6,8 kali dan memiliki stabilitas yang baikpada kondisi asam dan basa serta lama penyimpanan. Hasil karakterisasi dengan KLT danSpektrofotometri UV-Vis menunjukkan polaritas garam hasil sintesis meningkat dan terjadinyahipsokromik. Hasil karakterisasi dengan FTIR menunjukkan hilangnya gugus fungsi –CHalkana, –C=C dan terbentuknya gugus fungsi –C=O pada hasil sintesis.Kata kunci : ekstrak lada, piperin, kromatografi lapis tipis, spektrofotometri uv-vis, fouriertransform infrared.

PENGGUNAAN FTIR-ATR ZnSe (FOURIER TRANSFORM INFRA RED) UNTUK PENETAPAN KADAR KUERSETIN DALAM TEH HITAM (Camellia sinensis L.) Wiwin - Winingsih; Mursyida - Ulfa; O - Suprijana
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 5, No 1 (2016)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakKuersetin adalah senyawa yang memiliki efek antioksidan dan terdapat pada berbagai macam tumbuhan, salah satunya teh hitam. Pada penelitian ini kuersetin pada teh ditetapkan kadarnya dengan cara FTIR ATR ZnSe. Metode FTIR ATR (attenuated total reflectance) merupakan teknik FTIR sederhana dan dapat digunakan pada pengukuran sampel dalam bentuk padat dan cairan. ATR merupakan teknik yang sangat cepat dan hanya memerlukan sampel dalam jumlah yang sedikit. Sampel teh diekstraksi dengan pelarut etanol 96% secara sonikasi dan dihidrolisis menggunakan HCl 1N. Analisis kuantitatif didasarkan pada nilai absorbansi pada bilangan gelombang 1508 cm-1- 1513 cm-1yang merupakan nilai serapan gugus C=C pada kuersetin. Pembuatan kurva kalibrasi dilakukan dengan menggunakan metode penambahan standar kuersetin dengan lima variasi konsentrasi, yaitu 0,1%, 0,2%, 0,3%, 0,4% dan 0,5% ke dalam 50 mg sampel. Berdasarkan hasil perhitungan kurva kalibrasi diperoleh kadar kuersetin dalam sampel yaitu 0,186%. Kinerja analitis yang dilakukan diantaranya akurasi, presisi dan linearitas. Uji akurasi dilakukan pada konsentrasi teh yaitu 80% (40 mg sampel + 10 mg KBr), 100% (50 mg sampel), 120% (50 mg sampel + 10 mg kuersetin standar), ketiganya memenuhi parameter akurasi yang dipersyaratkan yaitu pada rentang 98% - 102%. Presisi dilakukan dengan menghitung RSD yaitu 1,72 memenuhi rentang nilai RSD ≤ 2,5. Uji linearitas dilakukan terhadap standar kuersetin dengan lima variasi konsentrasi, yaitu 0,1%, 0,2%, 0,3%, 0,4% dan 0,5% memiliki nilai koefisien korelasi (r) 0,93 dengan rentang konsentrasi 0,1 – 0,5%. Analisis kuersetin dengan metode FTIR-ATR ZnSe menunjukkan kinerja analitik yang baik dengan nilai akurasi dan presisi memenuhi standar validasi. Kata Kunci: Kuersetin, Teh Hitam, FTIR-ATR ZnSe AbstractQuercetin is an antioxidant agent that widely produced by plant, one of it is tea. In this research the determination of quercetin from black tea was carried out by FTIR ATR ZnSe method. FTIR ATR ZnSe techniques are simple and can be used in the measurement of the sample in the form of solids and liquids. ATR is a technique that is very fast and requires only small amounts of sample. The sample was extracted by sonication using ethanol 96% as the solvent and hydrolyzed by HCl 1N. The quantitative analysis was based on the absorbance value at wave number 1508 cm-1 to 1513 cm-1which was the absorbance of C = C group in quercetin. Calibration curve was made by standard addition method. The concentration of standard quercetin used was 0,1%, 0,2%, 0,3%, 0,4% and 0,5% in 50 mg of sample. The concentration of quercetin in the sample was calculated from the calibration curve and the result was o,186%. the performance of analytical method was determined by measuring accuracy, precision and linearity. Accuracy test carried out by using the concentration of tea 80% (40 mg sample + 10 mg KBr), 100% (50 mg sample), 120% (50 mg sample +10 mg standard quercetin), the result met the parameters required accuracy which is in the range of 98% - 102%. Precision was determined by calculating the RSD the result was 1,72,that was fulfill the required RSD value, which is RSD ≤ 2,5. Linearity test performed on quercetin standard with five variations of concentration, namely 0,1%, 0,2%, 0,3%, 0,4% and 0,5% which had the correlation coefficient (r) 0,93 in the concentration range of 0,1 – 0,5%. Analysis of quercetin with FTIR-ATR method showed good analytical performance based on the value of accuracy and precision that met validation standards requirement. Keywords: Quercetin, Black tea, FTIR-ATR ZnSe

PRODUKSI ASTAXANTHIN DARI MIKROALGA Haematococcus pluvialis MENGGUNAKAN EKSTRAKSI KARBON DIOKSIDA SUPERKRITIKAL YANG DIMODIFIKASI Adang - Firmansyah; Wiwin - Winingsih; Zenith Virgina Ababiel; Nella - Nurmeilasari; Ati - Setiasih
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 8, No 2 (2019)
Publisher : Sekolah Tinggi Farmasi Indonesia

Astaxanthin adalah pigmen karotenoid yang memiliki aktivitas antioksidan lebih tinggi dibandingkan dengan turunan β-karoten lainnya dan vitamin E. Sumber utama astaxanthin alami terdapat dalam mikroalga Haematococcus pluvialis yang dapat ditemukan di berbagai wilayah perairan di Indonesia. Astaxanthin alami dari H. pluvialis dapat diperoleh melalui proses isolasi. Sebagian besar metode isolasi yang telah diteliti menggunakan ekstraksi pelarut yang memiliki efek buruk pada lingkungan dan kesehatan, berbeda dengan ekstraksi superkritis CO2 yang memiliki efek baik pada lingkungan dan kesehatan. Pada penelitian ini, H. pluvialis dikultivasi, diinduksi dengan mengoptimalkan berbagai metode induksi stres, kemudian diisolasi dengan ekstraksi superkritis CO2 yang dimodifikasi menggunakan dry ice CO2 sebagai pelarut. Hasil penelitian menunjukkan bahwa H. pluvialis menghasilkan astaxanthin pada induksi cahaya matahari dengan panjang gelombang maksimum sesuai dengan produk standar yaitu 474 nm. Pengujian terhadap skema isolasi dan uji kesesuaian sistem menunjukkan bahwa pembentukan cairan superkritis dari dry ice CO2 ditentukan oleh massa, volume, dan suhu. Hasil dari uji pendahuluan kemudian dirancang sebuah ekstraktor dari bahan stainless steel dan hasil pengujian menunjukkan bahwa ekstraktor masih perlu dikembangkan, adapun isolasi yang dilakukan pada chamber simulasi menunjukkan tidak terjadi pemisahan.Kata Kunci: Astaxanthin, Haematococcus pluvialis, Ekstraksi CO2 Superkritis.

KAJIAN IN SILICO INTERAKSI ANTARA PIPERIN, MANGOSTIN, DAN KURKUMIN DENGAN KARAGENAN Wiwin Winingsih; Pebry Purnamasari
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 12, No 1 (2023)
Publisher : Sekolah Tinggi Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58327/jstfi.v12i1.175

Mangosteen, curcumin and piperine are compounds that have various pharmacological activities but have low solubility in water. To increase their solubility, several methods were available, one of it was inclusion complex formation. Inclusion complex consist of guest molecule and host molecule as complexing agent. Complexing agents that have been widely used was cyclodextrin, the use of carrageenan as a host for mangosteen, curcumin and piperine has never been reported . This research aimed to study the interaction between carrageenan with mangosteen, curcumin and piperine and compare it with cyclodextrins by in silico method. This research can be used to design inclusion complex of those three compounds with carrageenan. This study was carried out by molecular docking. USCF Chimera® 1.12 and Autodock® Vina software were used to study the interaction between mangosteen, curcumin and piperine as ligands with kappa carrageenan, iota carrageenan and beta-cyclodextrin as receptors. Docking results showed that these compound had good interaction with carrageenan as well as cyclodextrin by the formation hydrogen bonds, van der walls and ionic bonds.

Nutritional value and contamination testing assistance for the improvement of the processed food products quality Syarif Hamdani; Adang Firmansyah; Muhammad Ismail Faruqi; Dewi Astriany; Sri Gustini; Wiwin Winingsih; Melvia Sundalian; Nur Asni Setiani; Putri Afiyanti
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 8, No 4 (2023): November 2023
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v8i4.11295

Micro, Small, and Medium Enterprises (MSMEs) are one of the business sectors driving the country's economy. MSMEs produce many quality products that can compete in the market, where one of the products that is marketed quite a lot is food products. Several MSMEs in the Purwakarta Regency area have not included information on nutritional value and contamination on labels or packaging so these products cannot be widely marketed or traded in official stores and can only be marketed in their production areas. The Community Service activities carried out aim to carry out nutritional value and contamination tests, followed by improving product quality through workshops and mentoring. The food products targeted are simping and instant turmeric drinks produced by MSMEs in Pondoksalam District, Purwakarta Regency. Laboratory test results show that instant turmeric does not meet several SNI standard parameters, so quality improvement is still needed to be able to adapt it to become a product that meets standards. The results of the questionnaire show that several MSME owners in the Purwakarta area have a poor understanding of food product quality standards and ways to improve them. The coaching program for community service activities shows an increase in knowledge and understanding so that food products from Purwakarta MSMEs are expected to meet product quality standards in the future.

Analysis of Ethyl p-Methoxycinnamate from Kaempferia galanga L. Extract by High Performance Liquid Chromatography Wiwin Winingsih; Sri Gustini Husein; Rozalia Putri Neno Ramdhani
Journal of Tropical Pharmacy and Chemistry Vol. 5 No. 4 (2021): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v5i4.331

Ethyl para-methoxycinamate (EPMS) is a major compound of Kaempferia galanga L that has anti-inflammatory effect. The purpose of this study was to determine of EPMS in Kaempferiae galanga L rhizome extract by High Performance Liquid Chromatography (HPLC) and evaluated the performance of the analysis. This study included determination of system suitability, accuracy, precision, linearity and range, limit of detection (LOD) and Limit of quantitation (LOQ) and selectivity. The results of system suitability test HPLC System for EPMS analysis were as follows isocratic elution system of a mobile phase mixture of methanol: water (70:30) containing 0.1% TFA, uv detector at a wavelength of 308 nm using column C18 (150 × 4, 6mm, 5?m) flow rate 1 ml / min. From the analysis, it was found that the average EPMS content was 78.74%. Then method had linear concentration range from 5-360 ppm, with R ² = 0.9999. The LOD and LOQ were 7.0722 ppm and 21.4311 ppm respectively. The accuracy of this method that represented by % recovery was 98.02% - 101.26%. The precision of this method that expressed by Relative Standard Deviation (RSD) was 1.57%. The selectivity of this method that showed by resolution value was 2.6. Based on the results of the system suitability test and analysis performance evaluation,all parameters met the requirements.

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