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All Journal Jurnal Kesehatan Terpadu (Integrated Health Journal) Jurnal Info Kesehatan
Martsiningsih, Martha Atik
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology

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Liofilisasi Hemolisat Packed Red Cell Limbah Darah Palang Merah Indonesia dengan Penambahan Lioprotektan sebagai Alternatif Standar Hemoglobin Supriyanta, Bambang; Rahmawati, Ullya; Martsiningsih, Martha Atik; Pudyastuti, Rita Rena
Jurnal Kesehatan Terpadu (Integrated Health Journal) Vol 14 No 2 (2023): Jurnal Kesehatan Terpadu (Integrated Health Journal) November 2023
Publisher : Poltekkes Kemenkes Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32695/jkt.v14i2.509

Abstract

Lyophilization of PRC waste added with 20% sucrose as lyoprotectant. This study aimed to create an alternative Hb standard, replacing the manufacturer's Hb standard. This study used an experimental with pre and posttest without control group design. PMI blood waste PRC was processed into hemolysate, added with lyoprotectant, lyophilized, checked Hb levels using the Cyanmethemoglobin method as a grouppre test, stored at 4OC for six months, checked Hb levels with the Cyanmethemoglobin method as a grouppost test. Homogeneity Test and Stability Test were carried out according to ISO 13528:2015. The average Hb level of PRC lyophilisate before storage (Xr)=15.06 g/dl, obtained Ss≤0.3σpt, so that it was declared homogeneous according to the homogeneous acceptability value according to ISO 13528: 2015. After being stored for six months at 4OC, the average level of Hb lyophilisate PRC (Yr) =14,68 g/dl, obtained |Xr-Yr| ≤ 0.3σpt so that it was declared stable according to the stable acceptability value according to ISO 13528: 2015. Hb levels of PRC lyophilisate were stated to be homogeneous and stable for six months. Hb standard from PRC lyophilisate can be used as an alternative to commercial Hb standard.
Air Conditioner Condensate and Mineral Water as Alternative Solvents for Potato Dextrose Agar: Comparative Growth of Trichophyton rubrum Martsiningsih, Martha Atik; Yusmiarti, Yusmiarti; Nuryati, Anik; Azizah, Siti Nur; Kasiyati, Menik; Budi Setiawan
JURNAL INFO KESEHATAN Vol 24 No 1 (2026): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol24.Iss1.2239

Abstract

Trichophyton rubrum is the predominant cause of dermatophytosis worldwide, particularly in tropical countries such as Indonesia. Potato Dextrose Agar (PDA) is a standard medium for fungal culture, conventionally prepared with distilled water; however, its limited availability and high cost in resource-constrained laboratories necessitate alternative solvents. This study aimed to evaluate the growth of T. rubrum on PDA prepared with distilled water, air conditioner (AC) condensate, and bottled mineral water by comparing colony diameter and morphology. A quasi-experimental study with a post-test-only non-equivalent control group design was conducted. PDA was prepared using the three solvents, inoculated by the single-dot method, and incubated at a fixed temperature of 28°C for 7 days. Colony diameter and macroscopic and microscopic morphology were assessed. Statistical analyses included the Shapiro–Wilk test, One-Way ANOVA, and Independent Sample T-test (p < 0.05). All media supported typical T. rubrum growth. Colony diameters obtained using AC condensate were not significantly different from those obtained using distilled water, whereas mineral water resulted in significantly smaller colonies. These findings indicate that AC condensate can serve as an effective and economical substitute for distilled water in PDA preparation, while the use of mineral water should be limited due to variability in composition and lack of standardization.      
Co-Authors Anik Nuryati, Anik Bambang Supriyanta, Bambang Budi Setiawan Kasiyati, Menik Pudyastuti, Rita Rena Siti Nur Azizah, Siti Nur Ullya Rahmawati Yusmiarti, Yusmiarti