Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran dan Kesehatan Indonesian Journal of Biomedicine and Clinical Sciences
Perdana, Taufik Mulya
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Pengaruh Enzim Taq Polimerase dan Suhu Annealing terhadap Amplifikasi Gen Tropomyosin Sarcoptes scabiei Perdana, Taufik Mulya; Dwiputro, Alfin Harjuno; Wijaya, Yogik Onky Silvana; Satoto, Tri Baskoro Tunggul
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.138-145

Abstract

Skabies adalah penyakit kulit yang menjadi masalah kesehatan masyarakat, terutama di negara berkembang. Hingga saat ini, belum ada alat bantu diagnostik yang dapat digunakan untuk menunjang diagnosis skabies. Salah satu kandidat alat diagnostik yang gencar dikembangkan adalah uji imonologis yang mendeteksi antibodi anti-protein struktural dari Sarcoptes scabiei seperti tropomyosin. Selama ini, pembuatan tropomyosin rekombinan menggunakan sumber berupa copy DNA (cDNA) yang cenderung mahal. Untuk menekan beban produksi, digunakanlah genomic DNA (gDNA) sebagai sumber materi genetik. Akan tetapi, pembuatan tropomyosin rekombinan dari gDNA belum pernah dikerjakan. Oleh karena itu, dilakukanlah studi optimisasi polymerase chain reaction (PCR) ini sebagai langkah awal pengembangan alat bantu diagnostik tersebut. Penelitian ini bertujuan untuk mengetahui kondisi amplifikasi gen tropomyosin yang optimal dengan mempelajari efek penggunaan MyTaqTM HS Red Mix (MeridianBioscience) and GoTaq® Green Master Mix. (Promega) serta suhu annealing yang berbeda pada amplifikasi gen tropomiosin S. scabiei. Hasil PCR dengan MyTaqTM HS Red Mix pada suhu annealing 57.1°C, 60.9°C, 63.4°C, dan 65°C menghasilkan pita yang terlihat jelas. PCR dengan menggunakan GoTaq® Green Master Mix tidak menghasilkan amplifikasi DNA. Oleh karena itu, amplifikasi gen tropomiosin S. scabiei paling baik dilakukan dengan menggunakan MyTaqTM HS Red Mix, dengan suhu annealing 65°C.
Genotyping F1534C mutation on dried Aedes aegypti preparation through direct PCR method: a proof of concept Perdana, Taufik Mulya; Wijaya, Yogik Onky Silvana; Dwiputro, Alfin Harjuno; Najla, Aesha; Taftazani, Muhammad Rifqi; Insani, Dini Aura; Pangesti, Rachma Widya; Satoto, Tri Baskoro Tunggul
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.17975

Abstract

Aedes aegypti (Ae. aegypti) is the primary vector of dengue hemorrhagic fever (DHF). Various control strategies have been utilized to control its population, including the use of pyrethroid-based insecticides. However, the presence of mutations, such as the F1534C mutation, that confers resistance to pyrethroids has been increasingly reported. The increase of resistance-conferring mutation in Ae. aegypti population could potentially hinder DHF control measures. As such, monitoring the genotype of Ae. aegypti population is crucial. Mosquito rearing, DNA extraction, and PCR examination are usually employed to monitor the circulation of F1534C mutations. To simplify this process, we proposed a direct PCR workflow utilizing dried mosquito samples preserved on an in-house filter paper. To demonstrate the utility of our proposed workflow, we performed direct allele-specific PCR (AS-PCR) on 46 dried adult Ae. aegypti. As a comparator, conventional PCR was performed on 8 DNA extract from Ae. aegypti. Our results showed that direct AS-PCR successfully identified both wild-type (F allele) and mutant (C allele) genotypes from dried mosquitos with a success rate of 93.48%. These findings provide preliminary evidence supporting the use of cellulose-based in-house filter paper for genotyping insecticide-resistant mosquitoes. However, field testing must be performed before its implementation in real-world epidemiological and surveillance applications.
Co-Authors Dwiputro, Alfin Harjuno Insani, Dini Aura Najla, Aesha Pangesti, Rachma Widya Taftazani, Muhammad Rifqi Tri Baskoro Tunggul Satoto Yogik Onky Silvana Wijaya