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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kedokteran dan Kesehatan Indonesian Journal of Biomedicine and Clinical Sciences Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna (Parikesit)
Dwiputro, Alfin Harjuno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Prediction model of dengue hemorrhagic fever transmission to enhance early warning system in Gergunung Village, Klaten District, Central Java Satoto, Tri Baskoro Tunggul; Dwiputro, Alfin Harjuno; Risdwiyanto, Rifa Nadhifa; Hakim, A. Ulil Fadli; Pascawati, Nur Alvira; Diptyanusa, Ajib
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.788 KB) | DOI: 10.19106/JMedSci005103201909

Abstract

The dengue virus that causes dengue hemorrhagic fever (DHF) in principle is transmitted to humans through the bites of Aedes sp. In Indonesia, the disease is endemic in most provinces, including in Gergunung Village in Klaten District, Central Java. The village has shown the highest incidence of DHF for the last 5 years. Changes in demographical conditions, environment, and climate condition are predictors of dengue fever. This study aimed to demonstrate the association among human behavioral variables, physical environmental factors, and climate elements with DHF transmission to develop active surveillance model of DHF outbreak by the analysis of potential predictors. The research was an observational analytic study with case control design. Study population was selected from households with DHF case in 2016 through 2017 and the controls with ratio of 1:2. In total, 34 households were labeled as case and 68 households were labeled as control. Data collection was performed by observations, direct measurements, and interviews. Data were analyzed using appropriate statistical analysis with probability value of p<0.05. The result showed that insecticide use, proper waste management, livestock breeding, presence of plastered floor, water-resistant walls, bedroom windows, doors, gutters, and open drainage system, all did not show association with DHF case occurrence (p>0.05). In contrast, houses closer to each other tended to have more DHF cases (p<0.05; OR: 2.96; 95% CI: 1.01–8.67). Physical environmental factors and climate elements did not demonstrate significant associations with DHF case occurrence in this study. Human behavioral variables, physical environmental factors, and climate elements may serve as potential predictors of DHF outbreak, hence should be put into the model to enhance early warning system.
Pengaruh Enzim Taq Polimerase dan Suhu Annealing terhadap Amplifikasi Gen Tropomyosin Sarcoptes scabiei Perdana, Taufik Mulya; Dwiputro, Alfin Harjuno; Wijaya, Yogik Onky Silvana; Satoto, Tri Baskoro Tunggul
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.138-145

Abstract

Skabies adalah penyakit kulit yang menjadi masalah kesehatan masyarakat, terutama di negara berkembang. Hingga saat ini, belum ada alat bantu diagnostik yang dapat digunakan untuk menunjang diagnosis skabies. Salah satu kandidat alat diagnostik yang gencar dikembangkan adalah uji imonologis yang mendeteksi antibodi anti-protein struktural dari Sarcoptes scabiei seperti tropomyosin. Selama ini, pembuatan tropomyosin rekombinan menggunakan sumber berupa copy DNA (cDNA) yang cenderung mahal. Untuk menekan beban produksi, digunakanlah genomic DNA (gDNA) sebagai sumber materi genetik. Akan tetapi, pembuatan tropomyosin rekombinan dari gDNA belum pernah dikerjakan. Oleh karena itu, dilakukanlah studi optimisasi polymerase chain reaction (PCR) ini sebagai langkah awal pengembangan alat bantu diagnostik tersebut. Penelitian ini bertujuan untuk mengetahui kondisi amplifikasi gen tropomyosin yang optimal dengan mempelajari efek penggunaan MyTaqTM HS Red Mix (MeridianBioscience) and GoTaq® Green Master Mix. (Promega) serta suhu annealing yang berbeda pada amplifikasi gen tropomiosin S. scabiei. Hasil PCR dengan MyTaqTM HS Red Mix pada suhu annealing 57.1°C, 60.9°C, 63.4°C, dan 65°C menghasilkan pita yang terlihat jelas. PCR dengan menggunakan GoTaq® Green Master Mix tidak menghasilkan amplifikasi DNA. Oleh karena itu, amplifikasi gen tropomiosin S. scabiei paling baik dilakukan dengan menggunakan MyTaqTM HS Red Mix, dengan suhu annealing 65°C.
Peningkatan Pengetahuan dan Persepsi terhadap Penyakit Demam Berdarah Dengue pada Santri di Pondok Pesantren dengan Metode Ceramah Edukasi Tri Baskoro Tunggul Satoto; Dwiputro, Alfin Harjuno
Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna Vol 1 No 2 (2023): November
Publisher : Direktorat Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/parikesit.v1i2.9662

Abstract

Dengue hemorrhagic fever (DHF) is a vector-borne disease which posses a great threat for public health sector in many countries, including Indonesia. It is estimated more than 390 million cases are reported annually worldwide. Indonesia is one of the countries with the highest number of dengue cases in the world, with 95,000 cases recorded in 2018. Vector control is the main strategy for preventing the transmission of Dengue infection. Vector control practices are influenced by the level of public knowledge and perception. This study aimed to determine and improve the level of knowledge and perceptions of Islamic boarding school students regarding DHF transmission. This was a quasi-experimental research with one group pretest & posttest. The interventions carried out were in the form of health promotion with educational lectures on the prevention and transmission of DHF. The level of knowledge and perception was measured before and after the intervention using a questionnaire. The number of respondents in this study were 166 respondents, with most of them are female (50.6%). There was an increase in the average posttest scores compared to the pretest with respective averages of 63.84 ± 12.02 and 75.74 ± 11.78 (p = 0.0001). The existence of health promotion in the santri group could increase knowledge and perception of DHF as a vector-borne disease.
Genotyping F1534C mutation on dried Aedes aegypti preparation through direct PCR method: a proof of concept Perdana, Taufik Mulya; Wijaya, Yogik Onky Silvana; Dwiputro, Alfin Harjuno; Najla, Aesha; Taftazani, Muhammad Rifqi; Insani, Dini Aura; Pangesti, Rachma Widya; Satoto, Tri Baskoro Tunggul
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.17975

Abstract

Aedes aegypti (Ae. aegypti) is the primary vector of dengue hemorrhagic fever (DHF). Various control strategies have been utilized to control its population, including the use of pyrethroid-based insecticides. However, the presence of mutations, such as the F1534C mutation, that confers resistance to pyrethroids has been increasingly reported. The increase of resistance-conferring mutation in Ae. aegypti population could potentially hinder DHF control measures. As such, monitoring the genotype of Ae. aegypti population is crucial. Mosquito rearing, DNA extraction, and PCR examination are usually employed to monitor the circulation of F1534C mutations. To simplify this process, we proposed a direct PCR workflow utilizing dried mosquito samples preserved on an in-house filter paper. To demonstrate the utility of our proposed workflow, we performed direct allele-specific PCR (AS-PCR) on 46 dried adult Ae. aegypti. As a comparator, conventional PCR was performed on 8 DNA extract from Ae. aegypti. Our results showed that direct AS-PCR successfully identified both wild-type (F allele) and mutant (C allele) genotypes from dried mosquitos with a success rate of 93.48%. These findings provide preliminary evidence supporting the use of cellulose-based in-house filter paper for genotyping insecticide-resistant mosquitoes. However, field testing must be performed before its implementation in real-world epidemiological and surveillance applications.
Co-Authors Diptyanusa, Ajib Hakim, A. Ulil Fadli Insani, Dini Aura Najla, Aesha Pangesti, Rachma Widya Pascawati, Nur Alvira Perdana, Taufik Mulya Risdwiyanto, Rifa Nadhifa Taftazani, Muhammad Rifqi Tri Baskoro Tunggul Satoto Yogik Onky Silvana Wijaya