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Nutrition and Mental Health Empowerment Project for Counseling Teachers and Students of Senior High Schools in Yogyakarta Risky Oktriani; Dianandha Septiana Rubi; Yogik Onky Silvana Wijaya; Bira Arumndari Nurrahma; Mohamad Fay Faizal; Sarair Maulidya; Brilliant Alendra Adianfra; Irwan Supriyanto; Edilburga Wulan Saptandari; Arta Farmawati; Sunarti Sunarti
Journal of Community Empowerment for Health Vol 6, No 3 (2023)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.81891

Background: Mental disorders can affect people of any age, including teenagers of school age, which can disrupt their productivity and quality of life if not managed properly. There is growing evidence that nutrition is vital in mental health, which helps manage mental disorders. Simple interventions for mental disorders among teenagers through health promotion, education, and nutritional interventions are needed to prevent mental disorders or provide early treatment for those in need.Objective: Our project aimed to train counseling teachers and students of senior high schools in Yogyakarta to have the capability of performing simple management of mental disorders and promoting a healthy nutritional status.Methods: Our project consisted of three phases; phase I (webinar and workshop), phase II (practice and implementation at schools), and phase III (monitoring and evaluation). We used pre- and post-tests to assess respondents’ knowledge and questionnaires to assess their perceptions of the project.Results: Twenty counseling teachers and forty students from 20 senior high schools in Yogyakarta participated in this project. Our project slightly improved students' knowledge of the importance of nutrition and mental health but not the teachers’ knowledge. All participants welcomed our project positively. Most participants agreed they learned new and important information and stated that the project helped combat nutritional problems and mental disorders.Conclusion: Our project was perceived positively and improved participants’ skills and knowledge of nutrition and mental health issues. This suggests that the project has empowered them to perform basic management of nutritional problems and mental disorders among teenagers at their schools. Additionally, more projects are still needed to widen and maximize the coverage of these important topics.

Pengaruh Enzim Taq Polimerase dan Suhu Annealing terhadap Amplifikasi Gen Tropomyosin Sarcoptes scabiei Perdana, Taufik Mulya; Dwiputro, Alfin Harjuno; Wijaya, Yogik Onky Silvana; Satoto, Tri Baskoro Tunggul
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.138-145

Skabies adalah penyakit kulit yang menjadi masalah kesehatan masyarakat, terutama di negara berkembang. Hingga saat ini, belum ada alat bantu diagnostik yang dapat digunakan untuk menunjang diagnosis skabies. Salah satu kandidat alat diagnostik yang gencar dikembangkan adalah uji imonologis yang mendeteksi antibodi anti-protein struktural dari Sarcoptes scabiei seperti tropomyosin. Selama ini, pembuatan tropomyosin rekombinan menggunakan sumber berupa copy DNA (cDNA) yang cenderung mahal. Untuk menekan beban produksi, digunakanlah genomic DNA (gDNA) sebagai sumber materi genetik. Akan tetapi, pembuatan tropomyosin rekombinan dari gDNA belum pernah dikerjakan. Oleh karena itu, dilakukanlah studi optimisasi polymerase chain reaction (PCR) ini sebagai langkah awal pengembangan alat bantu diagnostik tersebut. Penelitian ini bertujuan untuk mengetahui kondisi amplifikasi gen tropomyosin yang optimal dengan mempelajari efek penggunaan MyTaqTM HS Red Mix (MeridianBioscience) and GoTaq® Green Master Mix. (Promega) serta suhu annealing yang berbeda pada amplifikasi gen tropomiosin S. scabiei. Hasil PCR dengan MyTaqTM HS Red Mix pada suhu annealing 57.1°C, 60.9°C, 63.4°C, dan 65°C menghasilkan pita yang terlihat jelas. PCR dengan menggunakan GoTaq® Green Master Mix tidak menghasilkan amplifikasi DNA. Oleh karena itu, amplifikasi gen tropomiosin S. scabiei paling baik dilakukan dengan menggunakan MyTaqTM HS Red Mix, dengan suhu annealing 65°C.

Kajian Manfaat Diet Mediterania: Analisis Loury Priskila; Tengku Arief Buana Perkasa; Yogik Onky Silvana Wijaya
Cermin Dunia Kedokteran Vol 51 No 11 (2024): Kedokteran Umum
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v51i11.1265

The rapid growth of nutrigenomics and nutrigenetics has greatly contributed to understanding the importance of genetic, nutrition, and environmental interaction in maintaining human health. Healthy lifestyles such as adequate physical activity and healthy diet patterns have been widely reported may reduce the risk of various diseases. One of the health diet patterns that has been widely studied is the Mediterranean diet (MedDiet). MedDiet is a plant-based diet pattern high in fiber, high in unsaturated fatty acids, and low in meat and dairy products. This literature study aims to summarize the health benefits of the MedDiet pattern and its biomechanisms.

HUBUNGAN POLIMORFISME MTHFR c.677C>T DENGAN FUNGSI KOGNITIF PADA PASIEN STROKE ISKEMIK AKUT DI RSUP DR. SARDJITO Haq, Arinal; Gofir, Abdul; Ar Rochmah, Mawaddah; Amelia Nur Vidyanti; Yogik Onky Silvana Wijaya, Yogik Onky Silvana Wijaya
Majalah Kedokteran Neurosains Perhimpunan Dokter Spesialis Saraf Indonesia Vol 40 No 2 (2024): Vol 40 No 2 (2024): Volume 40, No 2 - Maret 2024
Publisher : PERDOSNI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52386/neurona.v40i2.511

Introduction: Ischemic stroke is an acute cerebrovascular event with cognitive impairment presents as its prevalent manifestation and complication. Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme in folate metabolism through an integral process of cellular metabolism in DNA, RNA and protein methylation. MTHFR c.677C>T polymorphism is considered an important genetic risk factor for stroke and cognitive dysfunction in some populations. Aim: This study aimed to investigate the association between the MTHFR c.677C>T polymorphism and cognitive function in acute ischemic stroke patients in Dr. Sardjito General Hospital Yogyakarta. Methods: We performed a cross-sectional study in 42 consecutive acute ischemic stroke patients. PCR R-FLP was used to examine MTHFR c.677C>T polymorphism. Cognitive function was determined using MoCA-Ina within 24 hours of each patientÃ¢â‚¬â„¢s admission, with score 24 is the cut off for cognitive impairment. Results: Of 42 patients, 12 patients (28.6%) showed MTHFR c.677C>T variant. There were 3 patients (25%) with homozygous variant of MTHFR c.677C>T. Cognitive dysfunction was found in 7 patients (16.7%) with MTHFR c.677C>T variant and 18 patients (42.9%) with wild type MTHFR. However, no significant association was found between MTHFR c.677C>T with cognitive function in acute ischemic stroke patients (p=0.921). Discussion: The frequency of MTHFR c.677C>T polymorphism in this study was 28.6% with a quarter of them showing homozygous variant. There was no association between MTHFR c.677C>T polymorphism with cognitive function in acute ischemic stroke patients.

Genotyping F1534C mutation on dried Aedes aegypti preparation through direct PCR method: a proof of concept Perdana, Taufik Mulya; Wijaya, Yogik Onky Silvana; Dwiputro, Alfin Harjuno; Najla, Aesha; Taftazani, Muhammad Rifqi; Insani, Dini Aura; Pangesti, Rachma Widya; Satoto, Tri Baskoro Tunggul
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.17975

Aedes aegypti (Ae. aegypti) is the primary vector of dengue hemorrhagic fever (DHF). Various control strategies have been utilized to control its population, including the use of pyrethroid-based insecticides. However, the presence of mutations, such as the F1534C mutation, that confers resistance to pyrethroids has been increasingly reported. The increase of resistance-conferring mutation in Ae. aegypti population could potentially hinder DHF control measures. As such, monitoring the genotype of Ae. aegypti population is crucial. Mosquito rearing, DNA extraction, and PCR examination are usually employed to monitor the circulation of F1534C mutations. To simplify this process, we proposed a direct PCR workflow utilizing dried mosquito samples preserved on an in-house filter paper. To demonstrate the utility of our proposed workflow, we performed direct allele-specific PCR (AS-PCR) on 46 dried adult Ae. aegypti. As a comparator, conventional PCR was performed on 8 DNA extract from Ae. aegypti. Our results showed that direct AS-PCR successfully identified both wild-type (F allele) and mutant (C allele) genotypes from dried mosquitos with a success rate of 93.48%. These findings provide preliminary evidence supporting the use of cellulose-based in-house filter paper for genotyping insecticide-resistant mosquitoes. However, field testing must be performed before its implementation in real-world epidemiological and surveillance applications.

Kajian Manfaat Diet Mediterania: Analisis Priskila, Loury; Perkasa, Tengku Arief Buana; Wijaya, Yogik Onky Silvana
Cermin Dunia Kedokteran Vol 51 No 11 (2024): Kedokteran Umum
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v51i11.1265

The rapid growth of nutrigenomics and nutrigenetics has greatly contributed to understanding the importance of genetic, nutrition, and environmental interaction in maintaining human health. Healthy lifestyles such as adequate physical activity and healthy diet patterns have been widely reported may reduce the risk of various diseases. One of the health diet patterns that has been widely studied is the Mediterranean diet (MedDiet). MedDiet is a plant-based diet pattern high in fiber, high in unsaturated fatty acids, and low in meat and dairy products. This literature study aims to summarize the health benefits of the MedDiet pattern and its biomechanisms.

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