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All Journal SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Mercyska Suryandari, Mercyska
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Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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DETERMINATION OF TOTAL PHENOL CONTENT IN METHANOL EXTRACT OF ROSEMARY (Rosmarinus officinalis L.) LEAVES BY DIGETION METHODS USING UV-VIS SPECTROFOTOMETRY METHODS Mercyska Suryandari, Mercyska; Dwi Rahayu, Risma; Syafika Rani, Noor
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 3 No. 2 (2024): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62018/sitawa.v3i2.94

Abstract

The methanol extract of resemary leaves contains terpenoids, alkanoids, flavonoids, tannins, steroids, saponins and polyphenols. The aim of this research was to determine the total phenol of methanol extract of rosemary (Rosmarinus officinalis L.) by digestion method using the UV-Vis spectrophotometric method. In this study, methanol extract of rosemary leaves extracted using the digestion method was used as a sample and gallic acid as a standard. A standard solution of 200 ppm gallic acid was prepared to determine the operating time at 100 minutes and the maximum wavelength at 747 nm. Next, a comparison solution of gallic acid was made with a concentration range of 100, 125, 150, 175, 200 ppm and produced a linear regression equation, namely y = 0.0039× - 0.1146 with a correlation coefficient (r) of 0.9842. Determination of total phenol content was replicated 3 times and the absorption value was observed. The research results showed that the average total phenol content was 57.0317 mgGAE/g extract.
PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT 96% BLACK EAR MUSHROOM (Auricularia nigricans) BY SOXLETATION METHOD Mercyska Suryandari, Mercyska; Andhika Dwi Aristyawan, Andika; Fiska Yuliarni, Floreta; Ari Anggraini, Nony
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 3 No. 2 (2024): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Black ear mushroom (Auricularia nigricans) contains useful compounds, traditionally used as medicinal ingredients and food sources. The purpose of this study was to determine the secondary metabolites are contained in the extract of black ear mushroom (Auricularia nigricans). The extraction method used in this study is soxletation method. The sample used is 200 g of black ear mushroom (Auricularia nigricans) was extracted using soxhletation method with 1 l ethanol 96 % solvent. The phytochemical screenings test uses standard reagent by observing color changes, sediment, and foam. The result of the study that the extract of black ear mushroom (Auricularia nigricans) showed a red color after being given Dragendorrf reagent, terpenoid reagent test, flavonoid reagent test, gave a green color change after being given phenolic and tannin reagent test, did not give a red color in the steroid reagent test and did not produce foam in the saponin test. The conclusion of the study that the extract of black ear mushroom (Auricularia nigricans) contained secondary metabolite compounds tannins, terpenoids, flavonoids, phenolics, and alkaloids.
EFFECT OF EXTRACTION METHOD ON DETERMINING TOTAL PHENOL CONTENT OF ROSEMARY EXTRACT Mercyska Suryandari, Mercyska; Dwi Aristyawan, Andhika; Wefa, Ali; Ngurah Rio Juliana, I Made
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 4 No. 1 (2025): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62018/sitawa.v4i1.120

Abstract

Rosemary is a unique herb that is commercially available for use as an antioxidant. On the other hand, it can also be used as a food preservative, as it prevents oxidation and microbial contamination. Therefore, rosemary can be useful for replacing or even reducing synthetic antioxidants in food. Antioxidants are compounds that can inhibit oxidation reactions, by binding free radicals and molecules very effectively. As a result, cell damage will be inhibited. Known sources of antioxidants can be synthetic antioxidants and natural antioxidants originating from compounds contained in plants, one of which is phenolic compounds. To obtain phenolic compounds, extraction is carried out using maceration and digestion methods. The solvent choice here uses 80% ethanol solvent. The results obtained in this study were that 80% ethanol extract of rosemary was extracted using the maceration method, with a total phenol content of 11.84 mg GAE/g extract, while using digestion the total phenol content was 9.61 mg GAE/g extract. It can be concluded that the extraction method can influence the results of total phenol content with a significant difference with a p value <0.05
Co-Authors Ari Anggraini, Nony Aristyawan, Andhika Dwi Dwi Rahayu, Risma Ngurah Rio Juliana, I Made Syafika Rani, Noor Wefa, Ali Yuliarni, Floreta Fiska