Garuda - Garba Rujukan Digital

EKSTRAKSI JAMUR AURICULARIA DENGAN MENGGUNAKAN PELARUT ETANOL DAN METANOL Yuliarni, Floreta Fiska; Ayu Puji Lestari, Kinanti; Kun Arisawati, Diah; Dwi Winda Sari, Ratna; Ratna K., Kharisma
JURNAL TEKNOLOGI TECHNOSCIENTIA Technoscientia Vol 14 No 2 Maret 2022
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM), IST AKPRIND Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34151/technoscientia.v14i2.3637

The purpose of this study was to determine the characteristics of Auricularia extract which was extracted using 96% ethanol (sample E) and methanol (sample M). Auricularia was powdered before extraction. Extraction of mushrooms was carried out using the maceration method for three days. The solvents used for extraction were 96% ethanol and methanol. The extract that has been obtained is calculated by weight yield, tested for solubility, pH, and organoleptic tests. The yield weight of sample E was 1.47% higher than sample M, which was 1.22%. In the solubility test, sample E dissolved in water had a higher solubility of 60% compared to 96% ethanol, which was 55%, on the other hand, sample M dissolved in ethanol 96% had a higher solubility of 55% compared to 40% in water. Samples E and M dissolved in water had the same pH of 5, as well as those dissolved in 96% ethanol, which was 6. The organoleptic test on samples E and M had similarities in smell and shape, the difference was the color. The conclusion is samples E and M have almost the same characteristics.

EKSTRAKSI JAMUR AURICULARIA DENGAN MENGGUNAKAN PELARUT ETANOL DAN METANOL Yuliarni, Floreta Fiska; Ayu Puji Lestari, Kinanti; Kun Arisawati, Diah; Dwi Winda Sari, Ratna; Ratna K., Kharisma
JURNAL TEKNOLOGI TECHNOSCIENTIA Technoscientia Vol 14 No 2 Maret 2022
Publisher : Lembaga Penelitian & Pengabdian Kepada Masyarakat (LPPM), IST AKPRIND Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34151/technoscientia.v14i2.3637

The purpose of this study was to determine the characteristics of Auricularia extract which was extracted using 96% ethanol (sample E) and methanol (sample M). Auricularia was powdered before extraction. Extraction of mushrooms was carried out using the maceration method for three days. The solvents used for extraction were 96% ethanol and methanol. The extract that has been obtained is calculated by weight yield, tested for solubility, pH, and organoleptic tests. The yield weight of sample E was 1.47% higher than sample M, which was 1.22%. In the solubility test, sample E dissolved in water had a higher solubility of 60% compared to 96% ethanol, which was 55%, on the other hand, sample M dissolved in ethanol 96% had a higher solubility of 55% compared to 40% in water. Samples E and M dissolved in water had the same pH of 5, as well as those dissolved in 96% ethanol, which was 6. The organoleptic test on samples E and M had similarities in smell and shape, the difference was the color. The conclusion is samples E and M have almost the same characteristics.

Uji Antimikroba Daging Buah (Carica pubescens) Matang Terhadap Bakteri Staphylococcus aureus Metode Kirby Bauer Secara In Vitro Umarudin Umarudin; Floreta Fiska Yuliarni
SIMBIOSA Vol 8, No 2 (2019): JURNAL SIMBIOSA
Publisher : Universitas Riau Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33373/sim-bio.v8i2.2043

Staphylococcus aureus dapat menyebabkan infeksi kulit seperti bisul atau abses dan jerawat. Pengunaan antibiotik yang tidak tepat menyebabkan munculnya S. aureus yang resisten terhadap antibiotik karena adanya perubahan genetik. Permasalahan yang disebabkan akibat penggunaan antibiotik yang tidak tepat tersebut menimbulkan upaya eksplorasi senyawa antibakteri yang lebih efektif dengan dampak yang minimal. Salah satu tanaman yang bersifat sebagai antibakteri adalah daging buah karika (Carica pubescens). Penelitian ini dilakukan secara true eksperimental. S. aureus diperoleh dari laboratorium Universitas Airlangga dan diidentifikasi berdasarkan sifat biakan, pewarnaan Gram, uji biokimiawi dan uji gula-gula. Isolat selanjutnya diuji antimikroba terhadap ekstrak daging buah C. pubescens. Hasil penelitian menunjukkan bahwa bakteri yang tumbuh, dapat memfermentasi plat mannitol salt agar, sel berbentuk bulat bergerombol, bersifat Gram +, dapat memfermentasi maltosa dan laktosa, dapat menkoagulasi plasma kelinci dan bereaksi positif terhadap uji clumping faktor dan uji Voges Proskouer. Pada penelitian ini, hasil uji menunjukkan bahwa ekstrak daging buah Carica pubescens dengan konsentrasi 20%, 40%, dan 60% memiliki daya hambat terhadap Staphylococcus aureus. Daya hambat terbesar terdapat pada konsentrasi 60%. Semakin kecil konsentrasi ekstrak C. pubescens, semakin kecil daya hambatnya terhadap S. aureus.

Antioxidant Activity Test of Ubiquinone Microemulsion with DPPH (1,1-Diphenyl-2-picrylhydrazyl) Method : Uji Aktivitas Antioksidan Mikroemulsi Ubiquinone dengan Metode DPPH (1,1-Difenil-2-pikrilhidrazil) Silvi Ayu Wulansari; Umarudin; Floreta Fiska Yuliarni
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 7 No. 2 (2022): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v7i2.286

Ubiquinone is an antioxidant formulated in cosmetic preparations. It is necessary to test the atioxidant activity to ensure the quality of the preparation. The purpose of this study was to determine the antioxidant activity of the ubiquinone formulated in microemulsion preparations. The Ubiquinone were formulated into mikroemultion with cocentrations 0.25%, 0.5%, 0.75% and 1%, separately. The DPPH method was selected as the atioxidant activity test.. The data analysis in this study was descriptive to determine the IC50 value of ubiquinone microemulsion preparations F1, F2, F3 and F4 and ubiquinone as a comparison. The result shows that the antioxidant value of IC50 ubiquinone microemulsion of F1, F2, F3, and F4 are 56.48 ppm, 49.92 ppm, 32.76 ppm, and 20.71 ppm, respectively. The average value of ubiquinone microemulsion is 59.16 ppm. This research concludes that the microemulsion formula F1-F4 of ubiquinon has a very strong antioxidant activity compared to ubiquinone.

Antifungal Activity of Auricularia nigricans Methanol Extract against Candida parapsilosis using The Well Method : Aktivitas Antifungi Ekstrak Metanol Auricularia nigricans Terhadap Candida parapsilosis dengan Metode Sumuran Septiani Resika Dewi; Floreta Fiska Yuliarni
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 1 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i1.300

This study aims to determine the ability of the methanol extract of the fungus Auricularia nigricans to inhibit the growth of Candida parapsilosis. The extraction method used was maceration with the antifungal test method, namely the well method, the concentrations used were 0.2g/mL, 0.3g/mL, 0.4g/mL and 10% DMSO as a negative control. The results of the study showed that all concentrations used did not form inhibition zones. This result is included in the inactive category. So that Auricularia nigricans does not have antifungal activity against Candida parapsilosis.

Comparison and Evaluation of Fermentation Results of Probiotic Products by Acenobacter xylinum: Komparasi dan Evaluasi Hasil Fermentasi Produk Probiotik dengan Kultur Acenobacter xylinum Kinanti Lestari; Floreta Fiska Yuliarni; Lailatus Sa’diyah; Silvi Ayu Wulansari; Selvyronica Eka Agustine; Fatma Ariska Trisnawati; Silfiana Nisa Permatasari
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 1 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i1.334

products with Acetobacter xylinum starter included Nata and Kombucha. The large number of studies on probiotic product innovation should be followed by a survey on public acceptance of the products. This research aim to compare of the yield of probiotic products after fermentation process which was evaluated from a physical perspective, pH and public acceptance. This research is an experimental study to compared the fermented results of two types of probiotic products, nata and kombucha which are differentiated based on their raw ingredients. The nata products sample which had the largest thickness and weight of cellulose biofilm was NDC, while the kombucha products sample which had the largest thickness and weight of cellulose biofilm was KGF. pH of all samples, both nata and kombucha, decreased during the fermentation process. The samples of nata products that were most accepted by the panelists were NDC and NDM in terms of color, smell and taste. The samples of kombucha products that were most accepted by the panelists were KAF and KMF in terms of color, smell and taste.

Physical and Chemical Study of Manalagi Apple Peel (Malus sylvestris) Kombucha on 7 Days Fermentation Time: Studi Fisik dan Kimia Kombucha Kulit Apel Manalagi (Malus sylvestris) dengan Waktu Fermentasi 7 hari Kinanti Ayu Puji Lestari; Floreta Fiska Yuliarni; Widya Dara Anindya
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 2 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i2.358

KKombucha was one of the millennial beverage with a lot of benefits. Kombucha was a subject of research that was always interesting to discuss because of the various indicators of its subject. One of them was the Manalagi apple peel kombucha which was very rare on reviewed. This research focuses on the physical and chemical aspects of Manalagi apple peel kombucha. Manalagi apple peel kombucha is made in three different formulations namely FKA, FKB and FKC and fermented for 7 days. Each kombucha then went through a physical test which included thickness, wet weight, dry weight, moisture content and chemical evaluation as seen from changes in the pH of the kombucha using universal pH paper. The results showed that the physical evaluation of Manalagi apple peel kombucha with the FKC formulation had the highest thickness, wet weight, dry weight and yield, while the FKB formulation had the highest water content. While the pH of each formulation of kombucha apple skin Manalagi decreased and there were differences in values on the surface, middle and bottom of each formulation from pH 5 to pH 4 and 3.

Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families Yuliarni, Floreta Fiska; Sjamsuridzal, Wellyzar; Hidayat, Iman
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of Cercospora spp. infecting the plant families of Asteraceae, Cucurbitaceae, and Solanaceae were determined in this study. The sequences of 30 strains of Cercospora spp. infecting the plant families of Fabaceae, Amaranthaceae, Apiaceae, Plumbaginaceae, Malvaceae, Cistaceae, Plantaginaceae, Lamiaceae, and Poaceae were obtained from GenBank. The molecular phylogenetic analysis revealed that the majority of Cercospora species lack host specificity, and only C. zinniicola, C. zeina, C. zeae-maydis, C. cocciniae, and C. mikaniicola were found to be host-specific. Closely related species of Cercospora could not be distinguished using molecular analyses of ITS, EF, and CAL gene regions. The topology of the phylogenetic tree based on the CAL gene showed a better topology and Cercospora species separation than the trees developed based on the ITS rDNA region or the EF gene.

Pemanfaatan Air Leri dan Air Mikroorganisme Lokal Sebagai Pengendali Rayap Tanah Surahmaida, Surahmaida; Yuliarni, Floreta Fiska
Jurnal Teknologi Lingkungan Lahan Basah Vol 12, No 1 (2024): Januari 2024
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jtllb.v12i1.73006

Termites (Coptotermes curvignathus) are considered more dangerous than other termite species as they can cause significant damage to plantation and agricultural crops, with widespread distribution. The use of chemical pesticides to control this pest can lead to resistance and have negative impacts on the environment and human health. An environmentally friendly solution involves utilizing natural plants/substances as botanical insecticides, such as rice washing water and local microorganism (MOL) water. The aim of this research is to evaluate the effects of these two substances on termite population control and determine the optimal formulation that can act as a termite repellent. and to determine the optimal formulation as an anti-termite. The research methodology involves determining formulations of botanical insecticides, namely 100% rice washing water, 100% MOL water, 50% rice washing water : 50% MOL water, 75% rice washing water : 25% MOL water, 25% rice washing water : 75% MOL water. Each test box is filled 5 soldier termites and 20 worker termites, then provided with test papers that have been soaked in the predetermined formulation. Each treatment is repeated three times and observed every 24 hours for 72 hours. The research results indicate that all formulations uses did not have a significant impact on termite mortality. It can be concluded that the use of rice washing water and MOL water in the tested formulations is not effective as an insecticide to control termite.

PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT 96% BLACK EAR MUSHROOM (Auricularia nigricans) BY SOXLETATION METHOD Mercyska Suryandari, Mercyska; Andhika Dwi Aristyawan, Andika; Fiska Yuliarni, Floreta; Ari Anggraini, Nony
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 3 No. 2 (2024): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Black ear mushroom (Auricularia nigricans) contains useful compounds, traditionally used as medicinal ingredients and food sources. The purpose of this study was to determine the secondary metabolites are contained in the extract of black ear mushroom (Auricularia nigricans). The extraction method used in this study is soxletation method. The sample used is 200 g of black ear mushroom (Auricularia nigricans) was extracted using soxhletation method with 1 l ethanol 96 % solvent. The phytochemical screenings test uses standard reagent by observing color changes, sediment, and foam. The result of the study that the extract of black ear mushroom (Auricularia nigricans) showed a red color after being given Dragendorrf reagent, terpenoid reagent test, flavonoid reagent test, gave a green color change after being given phenolic and tannin reagent test, did not give a red color in the steroid reagent test and did not produce foam in the saponin test. The conclusion of the study that the extract of black ear mushroom (Auricularia nigricans) contained secondary metabolite compounds tannins, terpenoids, flavonoids, phenolics, and alkaloids.

Title

Found 16 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 16 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 16 Documents
Search