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All Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Hasil Hutan
Amatullah, Lutfiana Hary
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Materials Science & Nanotechnology

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Isolation and Molecular Identification of Proteolytic Bacteria from Rusip an Indonesian Fermented Food Amatullah, Lutfiana Hary; Afifah, Diana Nur; Jannah, Siti Nur
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.42237

Abstract

Processing fish with the fermentation method can increase the nutritional content, bring out a distinctive taste, and make the fish last longer. Rusip is a type of complementary food from Bangka Belitung in the form of fish sauce that has been rotten or fermented. Rusip is made from fermented anchovy (Clupeoides borneensis). The concept of fermentation in Rusip is the breakdown of complex protein molecules by proteolytic bacteria into simple amino acid components.The purpose of this study was to determine the presence of proteolytic bacteria that play a role in rusip fermentation.The media used in screening proteolytic bacteria is skim milk agar (SMA) media. Then it was incubated at 37oCfor 24 hours. Bacterial colonies that form a clear zone on the media indicate that these bacteria can degrade protein. After the isolation process and isolates were obtained, the bacteria were characterized by Gram staining method and molecular identification.The results showed that there were proteolytic bacteria in Rusip food, and the 3 best isolates that produced protease enzymes were identified as Bacillus cereus.
Potensi Asap Cair Tempurung Kelapa Sebagai Antijamur Fusarium Foetens, Fusarium Moniliforme, Dan Colletotrichum Capsici Purwantisari, Susiana; Sari, Dyah Maharani Sisya Puspita; Risnanda, Meizulfa Ayu; Khanifah, Neni Nur; Amatullah, Lutfiana Hary; Mahardhika, Wahyu Aji
Jurnal Penelitian Hasil Hutan Vol. 41 No. 2 (2023): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jphh.2023.998

Abstract

Fusarium and Colletotrichum are pathogenic fungi that attack potato and chili plants. Farmers often use synthetic fungicides to control the disease, while the continuous use of synthetic fungicides can cause negative impact to environment. This study aimed to test the effectiveness of plant disease control by using coconut shell liquid smoke. Inluded rejuvenation of isolates, characterization of tested isolates, and liquid smoke antifungal tests using the well diffusion method, and data analysis. Five formula concentrations were applied namely 0% (negative control), 10%, 20%, and 30% concentration, and antracol as positive control. The measurement of inhibition (mm) was carried out after 7 days of incubation. The result of in vitro effectiveness test showed that the liquid smoke had the ability to inhibit the growth of pathogenic fungi Fusarium foetens, F. moniliforme, and Colletotrichum capsici with the best concentration of 30%.
Co-Authors Diana Nur Afifah, Diana Nur Khanifah, Neni Nur Mahardhika, Wahyu Aji Risnanda, Meizulfa Ayu Sari, Dyah Maharani Sisya Puspita Siti Nur Jannah Susiana Purwantisari