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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Biologi Universitas Andalas Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Riset Biologi dan Aplikasinya Sciscitatio : Journal for Biological Science Jurnal Jeumpa Jurnal Penelitian Hasil Hutan Indonesian Journal of Science and Education
Mahardhika, Wahyu Aji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Catatan Jamur di Sekitar Hutan Danau Situgede (Bogor) dan Potensinya sebagai Sumber Pangan Mayra, Mahreza Tadsa; Siagian, Silsilia Lolabella Severina; Putri, Adisti Nursifa; Khairani, Dini; Santoso, Geitsha Zahira Shifa; Abdurrahman, Naufal Hafiz; Rhama, Mega Dwi; Mahardhika, Wahyu Aji; Sari, Okta Yulia; Putra, Ivan Permana
Jurnal Jeumpa Vol 11 No 2 (2024): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v11i2.10150

Abstract

Jamur merujuk pada semua jenis fungi yang berukuran makroskopis dan menghasilkan tubuh buah. Jamur umumnya memilih substrat yang bernutrisi sebab sifat hidupnya yang heterotrof. Kondisi lingkungan yang lembap dan intensitas cahaya matahari yang cukup dapat mendukung pertumbuhan jamurPenelitian ini bertujuan menentukan keanekaragaman dan potensi edibilitas jamur di sekitar Hutan Danau Situgede. Teknik pengumpulan data dalam penelitian ini menggunakan metode jelajah dengan pengambilan sampel menggunakan teknik sampling opportunis. Sampel segar kemudian diobservasi di lapangan untuk mengamati karakter makroskopis dan di laboratorium untuk mengamati karakteristik mikroskopik lanjutan. Karakter makroskopis dan mikroskopis kemudian dijadikan acuan dalam menentukan nama spesies berdasakan kunci identifikasi. Hasil penelitian didapatkan sebanyak empat jamur edible yaitu Auricularia auricula, Termitomyces microcarpus, Pleurotus ostreatus, dan Lentinus sp. serta satu jamur non-edible yaitu Microporus xanthopus. Seluruh jamur yang ditemukan berasal dari filum yang sama yaitu Basidiomycota. Hasil penelitian ini dapat berpotensi sebagai pilihan sumber pangan alternatif masyarakat dengan gizi yang tinggi dan manfaat farmakologi lainnya.
Biodiversity of Costus speciosus Phylloplane Fungi in Baturaden Botanical Gardens and Antagonist Testing against Fusarium oxysporum Aulia, Muhammad Hilman; Rukmi, Isworo; Suprihadi, Agung; Mahardhika, Wahyu Aji
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p41-50

Abstract

Indonesia has an abundant biodiversity of natural resources, one of which is phylloplane fungi. This abundant phylloplane mold needs further investigation to utilize its potential as an antagonistic agent. This study aims to prove the existence and to determine the types of phylloplane fungi species found on the leaf surface of pacing tawar (sweet ginger or crepe-ginger) plants, to analyze and determine the level of diversity and dominance of phylloplane, and to determine the types of phylloplane fungi species that have the antagonistic ability of the phytopathogenic mold Fusarium oxysporum. This research method includes isolating crepe-ginger phylloplane fungi using a contact plate, isolates purification, macromorphological and micromorphological identification, diversity and dominance index calculation, and antagonist test using a dual culture method. This study used a descriptive analysis research design. The isolation results obtained 57 species of crepe-ginger plants leaf phylloplane molds consisting of eight genera (Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Mucor, Penicillium, and Trichoderma). The results of the diversity index calculation with a value of 2.02 showed that the diversity of phylloplane fungi was moderate. The result of the dominance index calculation, with a value of 0.1521, shows no tendency for phylloplane fungi species to dominate. The results of the antagonist test showed that 15 isolates could antagonize the F. oxysporum with an inhibitory proportion of up to 82% obtained from T. viride isolates with code FD5B3.2
Identifikasi Morfologi Beberapa Jamur Liar di Kawasan Kampus IPB University, Bogor, Indonesia Harahap, Israwati; Mahardhika, Wahyu Aji; Putra, Ivan Permana
SCISCITATIO Vol. 5 No. 2 (2024): Volume 5 Number 2, January 2024 (Online First)
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.52.186

Abstract

Indonesia diketahui sebagai salah satu negara megabiodiversitas yang memiliki keberagaman tinggi termasuk jamur. Namun, sebagian besar publikasi terkait keragaman jamur di Indonesia tidak dilengkapi dengan deskripsi morfologi tubuh buah yang ditemukan. Eksplorasi dan deskripsi jamur sangat penting untuk mendata keberagaman jamur di Indonesia, tetapi data mengenai keanekaragaman jamur dengan data yang komperhensif masih sangat minim dilaporkan di Indonesia. Oleh karena itu, diperlukan eksplorasi jenis jamur di berbagai tempat, salah satunya adalah kawasan kampus IPB. Tujuan dari penelitian ini adalah untuk mengeksplorasi jamur yang terdapat di sekitar Kawasan kampus IPB guna menambah informasi keragaman jamur Indonesia. Eksplorasi jamur mengacu pada sampling opportunistik yang dilakukan dengan menjelajahi halaman Masjid Al-Huriyah dan hutan di sekitar perumahan dosen IPB berlokasi di Kecamatan Dramaga, Jawa Barat. Identifikasi jamur dilakukan dengan melakukan pengamatan secara makroskopis (dimensi dankarakter tubuh buah) dan mikroskopis (pileipellis, basidia, spora, hifa). Sebanyak 3 genus jamur berhasil diperoleh, diidentifikasi, dan dideskripsikan pada penelitian ini yaitu Geastrum sp., Auricularia sp., dan Psilocybe sp. Seluruh jamur tersebut merupakan anggota dari filum Basidiomycota dan kelas Agaricomycetes. Deskripsi Auricularia dan Psilocybe pada penelitian ini merupakan catatan baru untuk ragam jamur IPB dan berkontribusi pada pangkalan data jamur Indonesia.
Potensi Asap Cair Tempurung Kelapa Sebagai Antijamur Fusarium Foetens, Fusarium Moniliforme, Dan Colletotrichum Capsici Purwantisari, Susiana; Sari, Dyah Maharani Sisya Puspita; Risnanda, Meizulfa Ayu; Khanifah, Neni Nur; Amatullah, Lutfiana Hary; Mahardhika, Wahyu Aji
Jurnal Penelitian Hasil Hutan Vol. 41 No. 2 (2023): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jphh.2023.998

Abstract

Fusarium and Colletotrichum are pathogenic fungi that attack potato and chili plants. Farmers often use synthetic fungicides to control the disease, while the continuous use of synthetic fungicides can cause negative impact to environment. This study aimed to test the effectiveness of plant disease control by using coconut shell liquid smoke. Inluded rejuvenation of isolates, characterization of tested isolates, and liquid smoke antifungal tests using the well diffusion method, and data analysis. Five formula concentrations were applied namely 0% (negative control), 10%, 20%, and 30% concentration, and antracol as positive control. The measurement of inhibition (mm) was carried out after 7 days of incubation. The result of in vitro effectiveness test showed that the liquid smoke had the ability to inhibit the growth of pathogenic fungi Fusarium foetens, F. moniliforme, and Colletotrichum capsici with the best concentration of 30%.
Isolation and Identification of Entomopathogenic Fungi from Teak Leaf Little Soil and Paddy Soil with Various Isolation Methods Mahardhika, Wahyu Aji; Pitaloka, Gendis Angger; Nurcahyo, Rizky; Rukmi, Isworo; Suprihadi, Agung
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.228

Abstract

Abstrak Abstract Kapang entomopatogen merupakan kapang yang memiliki kemampuan untuk menginfeksi serangga. Kapang tersebut dapat menginfeksi dengan cara penembusan hifa ke tubuh serangga inang melalui kulit, saluran pencernaan, spirakel dan lubang lainnya. Tujuan dari penelitian ini yaitu untuk mengisolasi dan mengidentifkasi kapang entomopatogen pada tanah serasah daun jati dan tanah sawah dengan metode isolasi yang berbeda. Metode isolasi kapang entomopatogen menggunakan metode insect bait, insect bait dengan pengayakan tanah, dan metode pengenceran. Serangga yang digunakan untuk uji larva Tenebrio molitor.  Isolasi tanah sawah berhasil menumbuhkan kapang entomopatogen pada tubuh larva Tenebrio molitor ditandai dengan adanya miselium kapang menutupi tubuh larva. Hasil isolasi didapatkan 6 isolat yaitu  S2A, S2B, S2C, S3A, S3B, S3C. Keenam isolat tersebut merupakan kapang Fusarium sp.   Kata kunci: Fusarium sp., identifikasi, isolasi, kapang Entomopatogen, T. molitor Entomopathogenic fungi are molds that have the ability to infect insects. The mold can infect by penetrating the hyphae into the host insect's body through the skin, digestive tract, spiracles, and other holes. The purpose of this study was to isolat and identified entomopathogenic molds in teak leaf litter and paddy fields with different isolation methods. The isolation method of entomopathogenic molds used the insect bait method, insect bait with soil sieving, and the dilution method—insects used for the larval test of Tenebrio molitor. Isolation of paddy fields succeeded in growing entomopathogenic molds on the larval body of Tenebrio molitor, which was indicated by the presence of mold mycelium covering the larval body. The isolation results obtained 6 isolats, namely S2A, S2B, S2C, S3A, S3B, S3C. The six isolats were Fusarium sp.   Keywords: Entomopathogenic fungi, Fusarium sp., Identification, Isolation, T. molitor
Taxonomic Study and Bioprospecting Indonesian Termitomyces eurrhizus Ayu, Siti Maulyda; Mahardhika, Wahyu Aji; Putra, Ivan Permana; Nurhayat, Oktan Dwi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.289-297

Abstract

Termitomyces are known as wild edible mushrooms that are commonly consumed in Africa and Asia. T. eurrhizus can be easily found in many areas in Indonesia. However, the recent collection and proper documentation of this species has not been done in Indonesia. In addition, the potential utilization of this species needs to be revealed. This research aims to identify, investigate the proximate value, and test the antibacterial activity of T. eurrhizus. The exploration was carried out at the IPB University campus forest using an opportunistic sampling method. Fresh basidiomata were used for morphological identification. Proximate analysis was done following AOAC method. Mushroom extract was subjected to antibacterial test against Enteropathogenic Escherichia coli. Our specimen was identified as T. eurrhizus based on pileus characters (macroscopically) and spore features under an electron microscope (microscopically). The proximate result showed that the moisture content was 13,70 %, the ash 13,66 %, total fat content 2,05 %, total protein content 27,73 % and fiber content 8,49 %. The investigated antibacterial was evaluated based on size of antibacterial zone for concentration 200 mg/ml extract (3,5 mm), 100 mg/ml (6,5 mm), 50 mg/ml (3,83 mm), 25 mg/ml (2,5 mm), and 12.5 mg/ml 6,33 mm. Our result showed the potential utilization of T. eurrhizus as nutraceutical food and antibacterial. Further research is expected to collaborate with various cultivation or product development fields.
Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro Khanifa, Riskia; Ulya, Aida Himmatul; Darmawan, Ahmad Edi; Mahsunah, Anis H.; Mahardhika, Wahyu Aji
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.9655

Abstract

Whiteleg shrimp (Litopenaeus vannamei) is one of the most widely farmed shrimp species; however, its cultivation is frequently threatened by vibriosis caused by Vibrio parahaemolyticus. To date, the potential of antimicrobial peptides (AMPs) derived from Black Soldier Fly (BSF) larvae as natural antimicrobial agents has not been reported. This study aimed to evaluate the effectiveness of BSF larval AMPs against V. parahaemolyticus, the causative agent of vibriosis in L. vannamei. Data were analyzed using a quantitative descriptive approach. The results showed that BSF larval AMPs contained protein levels ranging from 5.2–12.03% in the first trial and 9.19–10.82% in the second trial, while the water content varied between 4.77% and 77.89%. In vitro testing demonstrated that BSF larval AMPs inhibited V. parahaemolyticus growth with a 9 mm inhibition zone in all treatments. Further in vivo studies and optimization are necessary to develop BSF larval AMPs as sustainable, eco-friendly antimicrobial agents.
Morphological Analysis for Identification and Classification of Fungal Species Diversity in Situ Gede Area, Dramaga, Bogor Ramadhani, Raihan; Mutmainah, Cintami Dewi; Putri, Nurlaila; Zahira, Saffana Restu; Mahardhika, Wahyu Aji; Putra, Ivan Permana
Indonesian Journal of Science and Education Vol. 8 No. 2 (2024): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijose.v8i2.1527

Abstract

Mushrooms are cosmopolitan organisms with very high biodiversity. Macro fungi, or mushrooms, are most commonly found in various regions of the tropical Earth. However, mushrooms in Indonesia have not been extensively explored and identified. Therefore, this research aims to identify several mushroom species found in the Situ Gede area, Bogor, West Java. Mushroom identification is carried out by collecting and measuring samples with a scale comparison in their natural habitat. The samples are then dried as a dry herbarium or soaked in alcohol as a wet herbarium. Four mushrooms were successfully identified, three of which belong to the phylum Basidiomycota and one belongs to the phylum Ascomycota. Some of these mushrooms include Lentinus arcularius, Lentinus sp., and Termitomyces microcarpus, which belong to the phylum Basidiomycota, while Xylaria polymorpha belongs to the phylum Ascomycota. Some fungi can be used as a source of food and also as a source of medicine.
Exploration of Pilobolus sp. Fungi from Various Livestock Manures in Kedung Pacul Village, Klaten Mahardhika, Wahyu Aji; Akmaliyah, Rizna; Diana, Milsa Solva; Listyowati, Sri
Bioma : Berkala Ilmiah Biologi Vol. 26, No 1, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.55874

Abstract

Pilobolus is a coprophilous fungus belonging to the Zygomycota. Pilobolus sp. is called a coprophilous fungus because it can live in animal feces. The uniqueness of this fungus is that it can shoot its spores, so Pilobolus sp. is called a shotgun fungus. Pilobolus sp. exhibits a phototropism mechanism in which the sporangium shoots spores in the direction of light. Pilobolus has a unique living habitat, namely in the manure of herbivores such as cows, goats, sheep, etc. This habit of life may seem terrible to us, but fungi like Pilobolus sp. are very important in life because they are a type of decomposer, capable of breaking down organic matter from dead living things. So the purpose of this study was to observe the spores produced by the fungus Pilobolus sp.  (a fungus in animal waste). The method used was to cultivate the fungus Pilobolus sp. for seven days on various animal wastes placed in jam bottles. The results showed that the Pilobolus sp. fungus grew on various media of animal feces, such as horses, cows, goats, and pigs, with different growth times, and there were also spores shot on the glass walls that had been perforated.
Eksplorasi Jamur Desa Tulung Agung, Lampung Timur dan Potensi Pemanfaatannya Sari, Okta Yulia; Mahardhika, Wahyu Aji; Afsari, Nadhila Mutia; Putra, Ivan Permana
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.105-119

Abstract

Mushrooms an be found in various places, such as litter, rotten wood, trees, and soil around residential areas. Tulung Agung Village in East Lampung Regency has various kinds of vegetation and several other abiotic factors that support the growth of mushroom. The study of mushroom’s exploration could elaborate the knowledge about mushroom diversity in resdential areas. This study aims to explore the types of mushrooms based on the description of their fruiting bodies from the residential location of Tulung Agung Village, District Pekalongan, East Lampung Regency, Lampung. The methods used were purposive sampling technique in a shady area or under the shade of a tree, documenting the mushroom fruiting bodies hat were found, and presenting mushrooms with fruiting bodies characters. After the exploration, 18 species from 14 genera of mushrooms were found, including Auricularia auricula-judae, Collybia sp., Conocybe sp., Daldinia sp., Gymnopilus sp., Marasmius sp., Marasmius haematocephalus, Mycena sp., Lepiota sp., Parasola sp., Pycnoporus sp., Trametes sp., Tremellodendron sp., and Xylaria sp. The various potentials of each type of mushrooms that were found are for food sources, traditional medicines, biodegradation of contaminants, sources of bioluminescence, and sources of various bioactive compounds. The Mushroom that were found need molecular identification to get the correct species so that the potential of Mushroom can be utilized optimally by residents based on more specific type information.
Co-Authors Abdurrahman, Naufal Hafiz Afsari, Nadhila Mutia Akmaliyah, Rizna Amatullah, Lutfiana Hary Arina Tri Lunggani Aulia, Muhammad Hilman Ayu, Siti Maulyda Chatarina Umbul Wahyuni Darmawan, Ahmad Edi Diana, Milsa Solva Hapzi Ali ISRAWATI HARAHAP Ivan Permana Putra Khairani, Dini Khanifa, Riskia Khanifah, Neni Nur Listyowati, Sri Mahsunah, Anis H. Mayra, Mahreza Tadsa MG Isworo Rukmi Mutmainah, Cintami Dewi Nurcahyo, Rizky Nurhayat, Oktan Dwi Pitaloka, Gendis Angger Putri, Adisti Nursifa Putri, Nurlaila Ramadhani, Raihan Ramadhany, Warih Rhama, Mega Dwi Risnanda, Meizulfa Ayu Santoso, Geitsha Zahira Shifa Sari, Dyah Maharani Sisya Puspita Sari, Okta Yulia Siagian, Silsilia Lolabella Severina Susiana Purwantisari Ulya, Aida Himmatul Zahira, Saffana Restu