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All Journal Jurnal Analis Kesehatan Medicra (Journal of Medical Laboratory Science/Technology)
Telleng, Prisca Audra
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Health Professions Immunology & microbiology Public Health

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Comparison of HCG Levels in Reagents Temperature 25°C and 8°C Using ELISA Method : Perbandingan Kadar HCG Pada Reagen Suhu 25°C dan 8°C Menggunakan Metode ELISA Telleng, Prisca Audra; Santosa, Budi; Kartika, Aprilia Indra
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1709

Abstract

HCG hormone is a hormone produced by the placenta in early pregnancy with the aim of maintaining pregnancy. The Elisa method is a method for examining HCG levels which has the principle of specific antigen and antibody reactions. The work stage in the laboratory that has the biggest error is in the pre-analytic stage reaching 60%, one of which is the reagent temperature. The ELISA method recommends the reagent temperature to be stored at 2-8°C and when used at 25°C. Examination reagents Elisa method uses reagents that use enzymes in the examination. Enzymes are very sensitive to temperature. This study aims to determine the ratio of HCG levels at reagent temperatures of 25°C & 8° using the ELISA method. This type of research is analytic supported by experiments and literature studies. Serum samples of 6 pregnant women were taken from the Kedungmundu Public Health Center using a limited population count formula. The examination was carried out by comparing the reagents at a temperature of 25°C and 8°C using 6 serums of pregnant women and in duplicate. Data analysis only used primary data and statistical tests were carried out using normality, homogeneity and T-Test tests. The average result at a temperature of 25°C was 380.851 and the average temperature of 8°C was 321.99 and the T-test statistic test showed p value = 0.469 and 0.470. Based on the results of the study, it can be concluded that there is an insignificant difference in the concentration of HCG by comparing the reagents at 25°C and 8°C.
Pemeriksaan Kadar hCG Metode ELISA pada Serum suhu 4°C dan 25°C Anggraini, Herlisa; Lestari, Erma; Kartika, Aprilia Indra; Kakiay, Grevanmoy Febriani; Telleng, Prisca Audra
Jurnal Analis Kesehatan Vol. 12 No. 2 (2023): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i2.4180

Abstract

Suhu serum pada uji ELISA merupakan faktor pra analitik yang dapat mempengaruhi kadar parameter yang diukur. Serum yang tidak diperiksa secara langsung akan disimpan pada suhu 4°C, sedangkan pemeriksaan ELISA menggunakan spesimen serum suhu ruangan (25°C). Masa tunggu suhu serum menjadi 4°C hingga 25°C seringkali tidak tepat sehingga pemeriksaan tetap dilakukan meskipun serum masih berada pada suhu 4°C. Untuk mengetahui perbedaan suhu serum pada suhu 4°C dan 25°C pada uji ELISA maka dilakukan penelitian dengan menggunakan parameter Human Chorionic Gonadrotopin (hCG). hCG adalah salah satu parameter untuk mendiagnosis kehamilan. Tujuan dari penelitian ini adalah untuk mengidentifikasi apakah terdapat perbedaan kadar hCG serum pada suhu 4°C dan 25°C menggunakan metode ELISA. Sampel yang digunakan adalah ibu hamil trimester II sebanyak 10 orang yang akan diperiksa secara duplo. Masing masing 10 spesimen akan diperiksa kadar hCG menggunakan metode ELISA dalam kondisi suhu serum 4°C dan 25°C. Hasil penelitian menunjukkan bahwa rata-rata hCG serum yang diperiksa pada suhu 25°C (385.677 IU/L) lebih tinggi dibandingkan dengan serum pada suhu 4°C (381.912 IU/L), namun hasil statistik menunjukkan tidak terdapat perbedaan yang signifikan antara kadar HCG diperiksa pada suhu 4°C dan 25°C (p >0,05).
Co-Authors Aprilia Indra Kartika, Aprilia Indra Budi Santosa Budi Santosa ERMA WIDYA LESTARI, ERMA Herlisa Anggraini Kakiay, Grevanmoy Febriani