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All Journal JPK: Jurnal Proteksi Kesehatan
Marshanda, Isty Maulia
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Health Professions Medicine & Pharmacology Nursing Public Health

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Antioxidant Activity Assay of Black Turmeric (C. Caesia) Water and Ethanol Extracted Using DPPH Kartini, Sri; Susanti, Pratiwi; Marshanda, Isty Maulia; Pratiwi, Denia; Mardhiyani, Dini; Hendrika, Yan
JURNAL PROTEKSI KESEHATAN Vol 12 No 2 (2023): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36929/jpk.v12i2.790

Abstract

Antioxidants are compounds that provide electrons; they function by donating an electron to oxidizing compounds, thereby inhibiting the activity of these oxidants. The objective of this study is to ascertain the presence of secondary metabolites and evaluate the antioxidant properties of water and methanol extracts obtained from black turmeric (C. caesia). The determination of antioxidant activity was conducted using the DPPH method with a microplate reader at a wavelength of 520 nm, by calculating the IC50 value. The research findings indicate that both water and methanol extracts contain flavonoids, saponins, and tannins. The antioxidant activity of the water extract measured at 392.47 ± 46.38 ppm (weaker), the methanol extract displayed a higher activity at 69.66 ± 2.91 ppm (strong), and Vitamin C exhibited very strong activity at 3.98 ± 0.26 ppm.
Co-Authors Hendrika, Yan Mardhiyani, Dini Pratiwi, Denia Sri Kartini, Sri Susanti, Pratiwi