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All Journal Jurnal Perlindungan Tanaman Indonesia Jurnal Entomologi Indonesia Mejuajua Prosiding Seminar Hasil Penelitian dan Pengabdian Kepada Masyarakat
Soffan, Alan
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Languange, Linguistic, Communication & Media Mechanical Engineering Public Health Other

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Journal : Jurnal Perlindungan Tanaman Indonesia

 1 
Molecular Analysis of Cytochrome Oxidase I in Bemisia tabaci (Gennadius) Populations Collected from Four Districts in the Special Region of Yogyakarta Ayuningtias, Nandini; Witjaksono, Witjaksono; Wijonarko, Arman; Soffan, Alan
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.75881

Abstract

Bemisia tabaci is an agricultural pest that interferes plant growth, as well as being an insect vector of various types of viruses, one of which is the geminivirus group. B. tabaci is called Cryptic Species Complex due to their similar morphology but has different genetic profiles. Climate change and increased global trading of agricultural products could increase B. tabaci population and lead to the emergence of genetic disparity. This study aims to obtain the latest information on the population homogeneity of B. tabaci in four districts of the Yogyakarta and potential differences on their nucleotide arrangements. Molecular identification was performed using PCR and primers C1-J-2198/L2-N-3914 on B. tabaci. B. tabaci COI gene sequences were then compared using a phylogenetic analysis and similarities of nucleotide bases were determined. Results showed that the populations of B. tabaci in Yogyakarta were still homogeneous showed by biotypes similarity, namely the non-B biotype. B. tabaci from Yogyakarta have nucleotide base similarity of 100% with B. tabaci from Singapore (AY686095) and Thailand (AY686092) and 99.56% with species from Bangladesh (AJ748388). Results also showed no differences in the composition of both nucleotide bases and amino acids from the four districts of B. tabaci sampling location. The homogeneous population of B. tabaci and the high incidence of yellowing disease caused by Begomovirus in chili pepper plants in the Special Region of Yogyakarta prove that it is necessary to review the current methods of controlling B. tabaci pests.
Feeding Behavior of Brown Planthopper (Nilaparvata lugens) on Pigmented Rice Monitored by Electrical Penetration Graph (EPG) Witjaksono, Witjaksono; Aldawood, Abdulrahman Saad; Kristamtini, Kristamtini; Sumarno, Sumarno; Andriyanto, Rofiq; Soffan, Alan
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.96909

Abstract

Rice is one of the essential daily commodity for most of Asian. However, the brown plant hopper (BPH; Nilaparvata lugens) infestation had been threatening the increasing demand of rice production. Evaluating resistance level of rice cultivars against BPH will help in managing BPH infestation. The feeding behavior monitoring of brown plant hopper (BPH; Nilaparvata lugens) by  using an electrical penetration graph (EPG) was conducted to evaluate the resistance level of several rice cultivars against BPH, including pigmented rice (black rice cv. Sembada hitam; red rice cv. Sembada merah); and the commonly consumed white rice (cv. Ciherang). The EPG instrument allowed the monitoring of BPH feeding behaviors by quantifying three unique waveforms, namely, N3, N4, and N5, which represent BPH feeding activities in areas near phloem tissues, in phloem tissues, and in xylem tissues, respectively. EPG monitoring of BPH feeding activities in black rice revealed the absence of the N3 and N4 waveforms. Red rice showed the N3 waveform but not the N4 waveform. White rice showed all three waveforms occurring with high numbers and long total durations. The absence of the N4 waveform in the two pigmented rice cultivars indicated the failure of BPH to access phloem tissues. Overall, the results revealed that the resistance of rice against BPH based on feeding activity could be ordered as follows: black rice > red rice > white rice. This report provides essential information on the resistance mechanism of pigmented rice cultivars against BPH.
Co-Authors Akbar, Muhammad Aulia Aldawood, Abdulrahman Saad Andriyanto, Rofiq Arman Wijonarko Ayuningtias, Nandini Budi Rahayu, Budi Claude Mona Airin Dwi Larasati Dwi Larasati, Dwi Elvika Fit Ari Shanti, Elvika Fit Ari Endah Puji Astuti, Endah Puji Fit Ari Shanti, Elvika Kristamtini Kristamtini, Kristamtini Okta Viyani, Ari Puji Astuti, Endah Sarmin Sarmin Sumarno . Tri Sunarsih Viyani, Ari Okta Witjaksono Witjaksono