Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Jaring SainTek
Riandani Azzahra, Syifa
Unknown Affiliation
Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Energy Engineering Industrial & Manufacturing Engineering

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Optimasi Ekstraksi DNA Pseudonocardia Carboxydivorans 18A213O1 Menggunakan Kit Ekstraksi Promega dan Qiagen Abdullah Setiawan, Wawan; Riandani Azzahra, Syifa; Handayani, Kusuma; Setiawan, Andi
Jurnal Jaring SainTek Vol. 6 No. 1 (2024): April 2024
Publisher : Fakultas Teknik, Universitas Bhayangkara Jakarta Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31599/js4jb268

Abstract

More than 70% of the earth's oceans are known to cover the surface of the earth. High biodiversity makes the ocean a habitat for microorganisms such as tunicates. Tunicates can associate with other microorganisms such as Pseudonocardia carboxydivorans so that they can produce bioactive compounds including antibiotics, pesticides and antitumors. The initial stage to identify a bioactive compound is by isolating DNA. This study aims to determine the optimization of bacterial DNA extraction using promega and qiagen extraction kits. This study consists of 3 treatments, namely rejuvenation, bacterial identification and concentration measurement. The results showed that the optimization of using qiagen extraction kit was relatively superior at 1,989 ng/µl - 2,000 ng/µl with 1 hour. While in the optimization of promega extraction, the resulting purity is 1,500 ng/µl - 1,943 ng/µl with a processing time of 2 hours.
Co-Authors Abdullah Setiawan, Wawan Andi Setiawan Kusuma Handayani, Kusuma