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Optimasi Ekstraksi DNA Pseudonocardia Carboxydivorans 18A213O1 Menggunakan Kit Ekstraksi Promega dan Qiagen Abdullah Setiawan, Wawan; Riandani Azzahra, Syifa; Handayani, Kusuma; Setiawan, Andi
Jurnal Jaring SainTek Vol. 6 No. 1 (2024): April 2024
Publisher : Fakultas Teknik, Universitas Bhayangkara Jakarta Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31599/js4jb268

Abstract

More than 70% of the earth's oceans are known to cover the surface of the earth. High biodiversity makes the ocean a habitat for microorganisms such as tunicates. Tunicates can associate with other microorganisms such as Pseudonocardia carboxydivorans so that they can produce bioactive compounds including antibiotics, pesticides and antitumors. The initial stage to identify a bioactive compound is by isolating DNA. This study aims to determine the optimization of bacterial DNA extraction using promega and qiagen extraction kits. This study consists of 3 treatments, namely rejuvenation, bacterial identification and concentration measurement. The results showed that the optimization of using qiagen extraction kit was relatively superior at 1,989 ng/µl - 2,000 ng/µl with 1 hour. While in the optimization of promega extraction, the resulting purity is 1,500 ng/µl - 1,943 ng/µl with a processing time of 2 hours.
Sosialisasi Pembuatan Pupuk Organik Cair Di Desa Pisang Indah, Bumi Agung, Way Kanan, Lampung Abdullah Setiawan, Wawan; Benyamin Timotiwu, Paul; Hendri, John; Setiawan, Andi
AMMA : Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2024): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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Abstract

The majority of people in Pisang Indah Village, Bumi Agung District, Way Kanan Regency, Lampung Province, are farmers. In farming, they use chemical fertilizers. Unfortunately, continuous use of chemical fertilizers can result in decreased soil quality and damage to soil structure. In order to replace chemical fertilizers with more affordable and ecologically friendly alternatives, socialization is being carried out on the manufacturing of Liquid Organic Fertilizer (Pupuk Organik Cair/POC) based on Local Microorganisms (Mikroorganisme Lokal/MOL). POC which comes from the fermentation of natural organic materials has become the main focus in efforts to increase plant productivity in a sustainable manner. This activity uses a descriptive-explanatory qualitative approach so that it presents practical steps to produce environmentally friendly and effective POC based on banana stems and rice washing water as an alternative fertilizer. The focus on the use of local organic materials, such as banana stems and rice washing water, is driving the adoption of sustainable agricultural practices in various agricultural communities. Through outreach activities and practical training, this article highlights farmers' interest and enthusiasm for POC as an alternative fertilizer that has the potential to provide good crop yields without damaging the environment. With the use of POC, it is hoped that in the future it can become the basis for efforts to empower farming communities in sustainable natural resource management to strengthen sustainable food security.
Pengenalan Fitotelmata Sebagai Tempat Perindukan Nyamuk Penular Penyakit Pada Masyarakat Desa Banjar Kanedi, Mohammad -; Sutyarso, Sutyarso; Rosa, Emantis; Abdullah Setiawan, Wawan
Jurdimas (Jurnal Pengabdian Kepada Masyarakat) Royal Vol 7, No 4 (2024): Oktober 2024
Publisher : STMIK Royal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33330/jurdimas.v7i4.3386

Abstract

In order to increase community participation in preventing the spread of mosquito-borne diseases, education for the community needs to be carried out through counseling. Tanggamus Regency is one of the areas in Lampung where DHF occurred, where from January to June 2024 there were 227 cases. To increase public knowledge in Banjar Agung Udik Village, Pugung District, Tanggamus Regency regarding phytotelmata as a breeding ground for mosquitoes, this counseling was carried out. Counseling was carried out using the pretest, lecture and discussion methods, and practicum. The results of the counseling showed that most participants knew that DBD and malaria were transmitted by mosquitoes, but not all knew the name of the vector species. Participants knew where mosquitoes perched at home and how to avoid mosquito bites. Participants also know mosquito breeding places, but are new to phytotelmata in this counseling. The practicum carried out by participants successfully identified six plant species that were proven to be phytotelmata. Thus, it can be concluded that this counseling has succeeded in providing knowledge to the community about the potential of phytotelmata as a source of mosquito-borne diseases.Keywords: Phytotelmata; Mosquito Borne Diseases; Mosquito Breeding Places; Mosquitoes Bite. Dalam rangka meningkatkan peran serta masyarakat dalam mencegah penyebaran penyakit tukar nyamuk itulah pendidikan kepada masyarakat perlu perlu dilakukan  melalui penyuluhan.  Kabupaten Tanggamus adalah salah satu daerah di Lampung yang kejadian DBD-nya dimana sejak Januari hingga Juni 2024 ada 227 kasus. Untuk meningkatkan pengetahuan masyarakat di Desa Banjar Agung Udik, Kecamatan Pugung, Kabupaten Tanggamus terhadap fitotelmata sebagai tempat perindukan nyamuk maka penyuluhan ini dilakukan. Penyuluhan dilakukan dengan metode pretes,  ceramah dan diskusi, serta praktikum. Hasil penyuluhan didapatkan bahwa  sebagian besar peserta tahu bahwa DBD dan malaria ditularkan nyamuk, tetapi tidak semua tahu nama spesies namuk vektornya. Peserta tahu tempat hinggap nyamuk di rumah dan juga  tahu  cara menghindarkan diri dari gigitan nyamuk. Peserta juga tahu tempat-tempat berkembang biak nyamuk, tetapi baru mengenal fitotelmata dalam penyuluhan ini. Praktikum yang dilakukan peserta berhasil mengidentifikasi enam spesies tumbuhan yang terbukti menjadi fitotelmata.Dengan demikian dapat disimpulkan bahwa penyuluhan ini berhasil memberkan pengetahuan kepada masyarakat tentang potensi fitotelmata sebagai sumber penyebar penyakit tular nyamuk.Kata kunci: Fitotelmata; Penyakit Tular Nyamuk; Tempat Perindukan Nymauk; Gigitan Nyamuk.
Edukasi Pengolahan Limbah Minyak Jelantah Menjadi Sabun Cuci Di Desa Gebang, Pesawaran, Lampung Handayani, Kusuma; Kanedi, Mohamad; Abdullah Setiawan, Wawan; Indriyani; Fadia, Yasmin
AMMA : Jurnal Pengabdian Masyarakat Vol. 4 No. 4 : Mei (2025): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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Abstract

Cooking oil contains fatty acids made from high-quality vegetable oil so it can be used for environmentally friendly food products. However, repeated heating processes can damage the molecular structure of the oil. So that it can cause several negative impacts such as on health if consumed too often and damage to the ecosystem in the surrounding environment if disposed of carelessly. To overcome these negative impacts, cooking oil waste can be processed into products such as laundry soap. This community service activity was carried out to educate the community, especially those in Gebang Village, Pesawaran Regency, about the dangers of repeated heating of cooking oil, as well as to provide alternatives to process it into more useful products. The results of this community service activity showed an increase in understanding for participants, this can be seen from the increase in the post-test scores worked on by participants and from the results of discussions during the activity.