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All Journal Veterinary Biomedical and Clinical Journal Jurnal Kedokteran Hewan
Amalia, Mifta Rizqina
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Isolation, Identification, and Antibiotic Susceptibility Test of Streptococcus spp. from a Cat Suspected of Respiratory Tract Disease at Nong Lam University, Vietnam Amalda, Elfahra Casanza; Amalia, Mifta Rizqina; Rifai, Ainrisq Auliya; Karo, Yohana Maria; Chintari, Lady Konfidenia; Palyoga, Habyb
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.1

Abstract

The nasal sample of a suspected respiratory tract disease cat, which is characterized by having a yellowish-colored nasal discharge, was obtained from a clinic in Ho Chi Minh City to be brought to the laboratory using the Stuart Amies transport medium. Samples were isolated on blood agar plate (BAP) media anaerobically. Gram staining and catalase tests were performed to identify the bacterial species. Furthermore, we conducted antibiotic sensitivity tests using Mueller-Hinton Agar (MHA) media, which included Tetracycline, Gentamicin, Marbofloxacin, and Amoxicilin-Clavulanate antibiotic disks. In order to prescribe an antibiotic, a clinical vet must go through a series of decision-making processes that involve both the drug and the host. In this case study, we were performing the diagnostic process before a medication could be prescribed to a patient with suspected respiratory tract disease.
THE USE OF TRITON X-100 ABSOLUTE WITH VARIATIONS OF IMMERSION TIME IN THE DECELLULARIZATION PROCESS OF INTESTINAL AND MYOCARDIAL XENOGRAFT Permata, Fajar Shodiq; Paramanandi, Dini Agusti; Nobilla, Nadia; Manalu, Rahel Oktarina; Amalia, Mifta Rizqina
Jurnal Kedokteran Hewan Vol 18, No 2 (2024): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i2.27712

Abstract

This study aims to determine the comparison of residual cells in the extracellular matrix of goat myocardium and intestinal segments after immersion in Triton X-100. A total of 20 goat myocardial segments (1x1x0.5 cm) and 20 goat intestinal segments (1x0.5x0.5cm) were divided into 4 treatment groups, namely the decellularization immersion groups for 24 hours, 48 hours, 72 hours and the control group without decellularization. In this research, the emersion technique was used by immersed the samples in 5 mL of absolute Triton X-100 solution. The samples were subjected to Hematoxylin-Eosin (HE) and Mallory staining. The HE staining showed a considerable number of nuclei remained in all of the treatment groups, in the control group, 24-hour group of treatment, 48-hour group of treatment and 72-hour group of treatment. An observation using Mallory staining showed an intact extracellular matrix in tissue samples following the immersion with absolute Triton X-100. Therefore, it can be concluded that the immersion using absolute Triton X-100 could not completely remove the extracellular matrix in the decellularization process until 72 hours of immersion.
Co-Authors Amalda, Elfahra Casanza Chintari, Lady Konfidenia Fajar Shodiq Permata Karo, Yohana Maria Manalu, Rahel Oktarina Nobilla, Nadia Palyoga, Habyb Paramanandi, Dini Agusti Rifai, Ainrisq Auliya