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All Journal Veterinary Biomedical and Clinical Journal Indonesian Educational Research Journal
Bello, Umar
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Effects of Acetone Extract of Green Tea (Camellia sinensis) on Diameter, Viability, and Germinal Vesicle Breakdown Rate of Zebrafish Oocytes (Danio rerio) Exposed to Heat Stress as an Animal Model Tuska, Habib Syaiful Arif; Hidayah, Aaliyah Nurul; Lonameo, Bonick Kartini; Bello, Umar; Budiono
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.2

Abstract

Global warming is characterized by increasing temperatures of the earth's atmosphere, ocean and land, resulting in extreme climate change. The effects of climate change will affect animal health through increased ambient temperature (heat stress). This study using Zebrafish as animal model like our previuous study because Zebrafish, have 70% identical genetic similarity to humans. In addition, Zebrafish have specific approval from the U.S. Food and Drug Administration for new drug discovery research. Heat stress negatively affects animal health through an increase in Reactive Oxygen Species (ROS), especially in the reproductive system because it causes a decrease in reproductive efficiency, such as follicular development, oocyte quality, and oocyte maturation. Antioxidant supplementation, namely green tea extract, is needed to reduce the negative effects of heat stress. The purpose of this study was to analyze the difference of 4 µl dose of green tea extract (Camellia sinensis) on the diameter, viability, and Germinal Vesicle Breakdown (GVBD) rate of Zebrafish oocytes at normal temperature (28℃) and with exposure to heat stress (32℃; 34℃). Zebrafish oocytes were collected and grouped into 2 (two) treatment groups including, non-heat stress group (28℃) and heat stress group (32℃; 34℃) with 6 repetitions.  Quantitative data were processed using SPSS software with Two Way ANOVA analysis.  The results of Two Way ANOVA showed that green tea extract supplementation had a positive effect on the diameter, viability, and GVBD of Zebrafish oocytes. This is indicated by the significance of the percentage of diameter (P=0.002), viability (P=0.003), and GVBD rate (P=0.000).
Pre-service Teachers Perception of the Use of E-learning During Covid-19 Pandemic in Colleges of Education in North-West, Nigeria Ismaila, Abdullahi Abubakar; Bello, Umar; Gambari, Isiaka Amosa
Indonesian Educational Research Journal Vol. 1 No. 3 (2024): Education system and implementation to improve various skills
Publisher : CV. Samuel Manurung and Co

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56773/ierj.v1i3.17

Abstract

This study of pre-service teachers’ perception of the use of e-learning during the Covid-19 pandemic in colleges of education in the North-west, Nigeria, was guided by two objectives; to find out the availability of e-learning tools in colleges of education in North-west, Nigeria during Covid-19, to investigate the perceived usefulness of e-learning by the pre-service teachers in colleges of education in North-west, Nigeria during Covid-19 and two research questions; are e-learning tools available for teaching and learning in colleges of education in North-west, Nigeria during Covid-19? How do pre-service teachers perceive the usefulness of e-learning in colleges of education? The researchers used mixed methods (quantitative and qualitative) for data collection.  A Multistage (called clustering) sampling technique was employed to select the sample from the colleges of education in North-west, Nigeria. The total population of the study is 113,030. The total sample that was chosen is 372 for quantitative and 23 for qualitative. The instruments used for data collection were adapted (questionnaires were used for quantitative, while data-blank was used for qualitative). The frequency and percentage were employed to analyze the respondents’ demographic information. The summary of the major findings points out that, there are no adequate e-learning resources in colleges of education in North-west, Nigeria that will enable them to use e-learning during Covid-19, and the pre-service teachers in colleges of education in North-west, Nigeria didn’t use to e-learn during Covid-19. The study affirmed that e-learning can be used as an instructional tool in colleges of education in North-west, Nigeria.
Co-Authors Budiono Gambari, Isiaka Amosa Hidayah, Aaliyah Nurul Ismaila, Abdullahi Abubakar Lonameo, Bonick Kartini Tuska, Habib Syaiful Arif