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All Journal Veterinary Biomedical and Clinical Journal Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary International Journal of Community Service
Tuska, Habib Syaiful Arif
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Socialization of The Using of Disinfectants In Cattle as an Effort To Prevent The Transmission of Foot And Mouth Diseases (FMD) In Sumber Rejeki, Dau, Malang Tuska, Habib Syaiful Arif; Orlona, Vanessa Prudence; Amri, Indah Amalia; Aini, Fidi Nur; Residiwati, Gretania
International Journal Of Community Service Vol. 3 No. 4 (2023): November 2023 ( Indonesia - Republik Demokratik Timor Leste - Malaysia - USA -
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijcs.v3i4.217

Abstract

Foot and Mouth Disease (FMD) is a contagious animal disease that attacks livestock. The Sumber Rejeki Dairy Cattle Group in Gading Kulon Village, Malang Regency has 356 cows of the Friesian Holstein breed. Several cows in Sumber Rejeki has been infected with the PMK virus. This disease causes a decrease in milk production in infected cows, causing economic losses to KSP breeders. Source of fortune. One of the efforts to prevent its spread is to disinfect the cage. Disinfection of cages is effective enough to kill the FMD virus because the FMD virus does not have an outer membrane and is easily destroyed at pH <5 and >7. Community knowledge regarding disinfection is still low, so socialization is carried out regarding the provision of disinfectants in cages. After the socialization, there was an increase in the knowledge of the farmers by 41.5% through the pretest and posttest which were given before and after the socialization.
Effects of Lycopene Treatments on Development, Hatchability, and Heart Rate of Zebrafish Embryos under Heat Stress Exposure Tuska, Habib Syaiful Arif; Salsabila, Odifiannisa Ayu; Lonameo, Bonick Kartini; Hardian, Andreas Bandang; Lesmana, M. Arfan; Yesica, Reza
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.1

Abstract

Lycopene, a potent antioxidant predominantly found in red-hued fruits and vegetables such as tomatoes, has been incorporated into embryonic maturation media in vitro, demonstrating enhancements in embryo quality. Despite these advancements, the specific effects of lycopene on Zebrafish embryo quality remain unexplored. This study aims to investigate the impact of lycopene (0.65 ppm) on the development of Zebrafish embryos, focusing on hatchability (HA), and heart rate (HR) under conditions of heat stress (HS). Fertile Zebrafish embryos at the gastrula stage were induced HS (30oC; 1 hour). The embryos will be segregated into two groups: those exposed to HS and nonHS (28oC). Each group will receive one of three treatments (four replicates): control / without lycopene (P1), 2μL lycopene (P2), and 4μL lycopene (P3). Observations using an inverted microscope was done every three up to 48 hours post-fertilization (hpf), and subsequently every five hours up to 96 hpf. Quantitative data obtained for HA and HR were analyzed with SPSS, One-Way ANNOVA (p<0.05). The antioxidants contained in lycopene can influence the speed and success of Zebrafish embryo development and HA exposed to HS. In addition, the antioxidant in lycopene can also suppress the HR frequency of Zebrafish embryos exposed to HS. However, more studies about the optimum dose, purity, and stability of the lycopene extract should be done to make a definitive conclusion.
Effects of Acetone Extract of Green Tea (Camellia sinensis) on Diameter, Viability, and Germinal Vesicle Breakdown Rate of Zebrafish Oocytes (Danio rerio) Exposed to Heat Stress as an Animal Model Tuska, Habib Syaiful Arif; Hidayah, Aaliyah Nurul; Lonameo, Bonick Kartini; Bello, Umar; Budiono
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.2

Abstract

Global warming is characterized by increasing temperatures of the earth's atmosphere, ocean and land, resulting in extreme climate change. The effects of climate change will affect animal health through increased ambient temperature (heat stress). This study using Zebrafish as animal model like our previuous study because Zebrafish, have 70% identical genetic similarity to humans. In addition, Zebrafish have specific approval from the U.S. Food and Drug Administration for new drug discovery research. Heat stress negatively affects animal health through an increase in Reactive Oxygen Species (ROS), especially in the reproductive system because it causes a decrease in reproductive efficiency, such as follicular development, oocyte quality, and oocyte maturation. Antioxidant supplementation, namely green tea extract, is needed to reduce the negative effects of heat stress. The purpose of this study was to analyze the difference of 4 µl dose of green tea extract (Camellia sinensis) on the diameter, viability, and Germinal Vesicle Breakdown (GVBD) rate of Zebrafish oocytes at normal temperature (28℃) and with exposure to heat stress (32℃; 34℃). Zebrafish oocytes were collected and grouped into 2 (two) treatment groups including, non-heat stress group (28℃) and heat stress group (32℃; 34℃) with 6 repetitions.  Quantitative data were processed using SPSS software with Two Way ANOVA analysis.  The results of Two Way ANOVA showed that green tea extract supplementation had a positive effect on the diameter, viability, and GVBD of Zebrafish oocytes. This is indicated by the significance of the percentage of diameter (P=0.002), viability (P=0.003), and GVBD rate (P=0.000).
Use of vitamin E and selenium injections as supportive treatment of retained placenta in dairy cattle Rahim, Nurul Insyirah; Hendrawan, Viski Fitri; Tuska, Habib Syaiful Arif; Agustina, Galuh Chandra
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.166-171

Abstract

The placenta is considered retained when it cannot separate from the uterus beyond the normal time limit. Physiologically, a cow's placenta comes out 3-8 hours postpartum. This study aims to determine the diagnosis and treatment of retained placenta in dairy cows using vitamin E and selenium as supportive treatment. On physical examination the patient had a body temperature of 39.9°C, was weak, unable to stand, and had decreased appetite. General examination in this case showed the following clinical symptoms: the placenta was still hanging from the vulva and there was an unpleasant odor from the vulva area. The retained placenta was removed manually, followed by flushing the uterus with 0.2% povidone iodine (in water). Antibiotic (Colibact bolus) were introduced into the uterus and penicillin-streptomycin was injected intramuscularly along with vitamin E and selenium as supportive treatment. The results showed that the cow recovered and returned to estrus 14 days after therapy. It could be concluded that injections of vitamin E and selenium helped restore the estrous cycle in cows with retained placenta.
Effect of Artemisia vulgaris Supplementation on Zebrafish Embryo Under Heat Stress Condition during In Vitro Culture Tuska, Habib Syaiful Arif; Khamalt, Nursalsabila; Lesmana, Muhammad Arfan; Yesica, Reza; Hendrawan, Viski Fitri; Budiono; Residiwati, Gretania
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.20-25

Abstract

Background: Artemisia vulgaris contains flavonoids, which play a vital role in counteracting free radicals. Purpose: To determine the effect of Artemisia vulgaris extract supplementation on embryo development, heart rate and survival of zebrafish under heat stressed and non-heat stressed conditions. Methods: The research used a completely randomized design. Zebrafish embryos (n=240) were divided into heat stressed (36°C) and non-heat stressed (28°C) groups, while for each group were divided into three subgroups, namely T1/control (without Artemisia vulgaris supplementation); and supplemented group T1 and T2, with 2 µL and 4 µL of Artemisia vulgaris supplementation, respectively. The efficacy of Artemisia vulgaris supplementation was determined by observing the embryo development, heart rate, and survival rate of zebrafish up to 96 hours post fertilization (hpf). Results: The development of zebrafish embryos under heat stressed treated with Artemisia vulgaris extract gave the same quality as the control treatment without heat stressed exposure. Zebrafish embryos exposed to heat stressed with 4 µL Artemisia vulgaris supplementation gave the highest survival rate on the heat stressed group. Artemisia vulgaris supplementation improved the heart rate of zebrafish exposed to heat stressed as in the non-heat stressed group. Conclusion: Artemisia vulgaris extract can reduce the detrimental effects of heat stressed induction on zebrafish embryos, as evidenced by the improvement in embryonic development, heart rate, and survival rate of zebrafish embryos after supplementation.
Identification, Prevalence Estimation, and Mapping of Coccidiosis in Broiler Chickens (Gallus domesticus) Across Varying Elevations in Malang Regency and Batu City Irawan, Heri; Hanifa, Filza Hannani Vawzia; Kusumarini R, Shelly; Tuska, Habib Syaiful Arif; Yesica, Reza
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.76540

Abstract

Broiler chickens are chickens that have been specifically bred for high meat production. The increase in chicken meat consumption has become both an opportunity and a challenge for farmers, one of which is coccidiosis. Coccidiosis is caused by the Eimeria protozoa, which commonly infect chickens and target their digestive tract. This study aims to calculate the proportion and identify Eimeria species that often cause coccidiosis in broiler chickens in Malang Regency and Batu City. A total of 180 fecal samples from 6 different farms were used. The examination was conducted using the McMaster method and sporulation for 2-7 days on positive samples by immersing feces in a 2% potassium dichromate solution. Identification of Eimeria species was based on morphology and morphometry and was then were analyzed using descriptive statistics. The relationship between risk factors and coccidiosis occurrence was analyzed using Chi-square. The distribution of coccidiosis infections in Malang was depicted on an epidemiological map. The results showed that there were 4 Eimeria species infecting the six subdistricts in Malang Regency and Batu City, namely E. necatrix, E. acervulina, E. praecox, and E. maxima. The proportion of coccidiosis incidence in Malang Regency and Batu City was 18.89%. The highest proportion was in Dau District (43.33%), followed by Pagelaran District (36.67%), Kromengan District (23.33%), Wagir District (6.67%), Junrejo District (3.33%), and lastly Batu District (0%). Chi-square analysis showed that altitude and temperature had a significant effect (p<0.05) on the coccidiosis incidence rate in Malang Regency and Batu City.
Co-Authors Agustina, Galuh Chandra Amri, Indah Amalia Bello, Umar Budiono Dameanti, Fidi Nur Aini Eka Puji Hanifa, Filza Hannani Vawzia Hardian, Andreas Bandang Hidayah, Aaliyah Nurul Irawan, Heri Khamalt, Nursalsabila Kusumarini, Shelly Lesmana, M. Arfan Lesmana, Muhammad Arfan Lonameo, Bonick Kartini Orlona, Vanessa Prudence Rahim, Nurul Insyirah Residiwati, Gretania Salsabila, Odifiannisa Ayu Viski Fitri Hendrawan Yesica, Reza