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Perbandingan Rendemen, Skrining Fitokimia Dan Profil Kromatografi Lapis Tipis Ekstrak Etanol 96% dan Metanol Daun Sungkai (Peronema canescens Jack) M. Andi Chandra; Hidayatullah, M. Hidayatullah; Putri, Putri Ermina Sari
Jurnal Kesehatan Islam : Islamic Health Journal Vol. 12 No. 2 (2023): Jurnal Kesehatan Islam : Islamic Health Journal
Publisher : Publikasi oleh Fakultas Kedokteran Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jki.v12i2.20818

Abstract

Abstract. Kalimantan is rich in plants that are used as medicinal plants, one of which is the sungkai plant (Peronema canescens Jack). The aim of this research was to determine the comparison of yields of different solvent extracts and the comparison of phytochemical screening results and TLC profiles of 96% ethanol and methanol extracts. Extraction was carried out by maceration, then phytochemical screening and stain profiles were carried out using the thin layer chromatography (TLC) method using the mobile phase N-Hexane and Ethyl Acetate (8:2). The results of the research showed that the ratio of % yield of 96% ethanol extract was 8.87% and methanol was 8.85%. The results of phytochemical screening with 96% ethanol identified more secondary metabolites than maceration using methanol. The conclusion in this study is that the % yield ratio of ethanol extract is 96% greater than methanol extract. Phytochemical screening had more secondary metabolites in the 96% ethanol extract compared to methanol and TLC analysis of the 96% ethanol extract of sungkai leaves produced 5 stains with Rf values ​​of 0.06, 0.2, 0.25, 0.31 and 0.41, extract methanol produced 6 stains with Rf values ​​of 0.05, 0.13, 0.21, 0.25, 0.45 and 0.58. Kata Kunci: Sungkai (Peronemacanescens Jack,) TLC, 96% Ethanol Extract: Methanol
PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETIL ASETAT BUAH OKRA HIJAU (Abelmoschus esculentus L.) Rahmi , Rahmi Muthia; Fatmawati, Fatmawati; Hidayatullah, M. Hidayatullah; Putri, Putri Indah Sayakti; Aditya, Aditya Noviadi Rakhmatullah
Jurnal Kesehatan Islam : Islamic Health Journal Vol. 12 No. 2 (2023): Jurnal Kesehatan Islam : Islamic Health Journal
Publisher : Publikasi oleh Fakultas Kedokteran Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jki.v12i2.20820

Abstract

Abstract. Okra fruit (Abelmoschus esculentus L.) is a potential medicinal plant because it contains several compounds such as alkaloids, phenols, flavonoids, steroids and tannins which have anti-diabetic, antioxidant, antimicrobial, antiallergic, antiviral and anti-inflammatory properties. The purpose of this study was to identify the content of flavonoid compounds qualitatively and quantitatively. The extraction method was maceration, used ethyl acetate solvent. Qualitative testing of flavonoid compounds used TLC, 5% AlCl₃ spotting appearance. Quantitative test used UV-Vis Spectrophotometry method, quercetin as comparison, λmaks 415 nm. The TLC results show three stains with each Rf value of 0.76; 0.53; 0.23. The results of determined the total flavonoid content of okra fruit were 4.069 ± 0.211 mg QE/g extract. From the research results obtained, it can be concluded that the ethyl acetate extract of green okra contains flavonoids. Kata Kunci: Abelmoschus esculentus L; total flavonoid content; ethyl acetate extract; TLC