Patient safety is a top priority in healthcare and nursing services. A strong patient safety culture plays a crucial role in preventing medical incidents that could endanger patients. The successful implementation of a safety culture in hospitals depends on the perceptions and practices of healthcare workers, particularly nurses. A well-implemented safety culture has positive impacts, such as reducing medical incidents, improving service quality, and increasing patient trust. However, suboptimal or inconsistent implementation may lead to negative effects, such as undetected medical errors, decreased compliance with procedures, and a weakened incident reporting culture. Therefore, continuous evaluation of safety culture implementation is essential. Objective: This study aims to analyze the influence of safety culture on patient safety among nurses at RSUD I. A Moeis Samarinda. This research used a quantitative approach with an analytical survey design. Data were collected through questionnaires distributed to nurses at RSUD I. A Moeis Samarinda, with samples selected using a convenience sampling technique. Statistical tests were conducted to identify the relationship between safety culture and patient safety. The study showed a significant relationship between safety culture and patient safety. Based on the Wilcoxon test, the p-value was 0.02 ≤ 0.05, indicating that the null hypothesis was rejected. Additionally, the Cronbach’s alpha value was greater than 0.6 (0.601 > 0.6), suggesting that safety culture has a positive and significant effect on patient safety at RSUD I. A Moeis Samarinda. Hospitals need to enhance nurses’ awareness of the importance of safety culture through training, optimal workload management, and the implementation of more effective safety policies.