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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi
Febriarti, Bernadeta Leni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Endophytic bacteria isolated from stems and roots of Acrostichum aureum Linn. potential for hydrolytic enzyme and α-amylase inhibitor Linda, Tetty Marta; Defani, Syafiqah Amirah; Berliansyah, Azizul; Febriarti, Bernadeta Leni; Zul, Delita
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.41668

Abstract

Endophytic bacteria live symbiotically in plant tissues but do not hurt plants. Endophytic bacteria are widely used in the industrial sector as enzyme producers. This study aims to examine the potential of endophytic bacteria from the stems and roots of sea fern (Acrostichum aureum L.) as producers of hydrolytic enzymes and to determine their potential as α-amylase inhibitors. Macroscopic and biochemical tests characterized endophytic bacterial isolates. Hydrolytic enzyme activity test consisted of cellulase, lipase, and laccase enzyme. Isolates that were able to hydrolyze were tested for antidiabetic potential by α-amylase inhibitor test. A total of 24 bacterial isolates were selected for their ability to produce cellulase, lipase, and laccase. The results obtained 24 isolates of endophytic bacteria showed that as much as 33% of stem isolates and 52% of root isolates were able to produce hydrolytic enzymes. The α-amylase inhibition test results of the three endophytic bacteria tested were isolates A.T 2 (17%) and A.A 3 (8%) on 1% starch substrate, and A.T 2 (36%) on 2% starch substrate with a spectrophotometer at a wavelength of 540 nm. Endophytic bacteria isolated from the stems and roots of sea ferns can be developed as an alternative base material for herbal medicines for antidiabetics.
The Ureolytic Soil Bacteria Bacillus albus, a potential Agent for Biocement Linda, Tetty Marta; Rahmani, Syauqi Susana; Wati, Andini Saras; Febriarti, Bernadeta Leni; Futra, Dedi; Olivia, Monita; Juliantari, Erwina
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.829-839

Abstract

Concrete is a common building material and is very vulnerable to cracking caused by unstable temperature/humidity. Concrete crack repair can be done by using microorganism substitution that can produce CaCO3 (calcite) compounds that can be used as an environmentally friendly method in improving structural formation and increasing the strength and durability of concrete, one of which is using ureolytic bacteria. This study aimed to isolate and characterize ureolytic bacteria isolates and then to assess the calcite precipitation potential of ureolytic bacteria isolates from landfills. The ureolytic bacterial isolates were grown on NB-U/Ca and tap water medium. Analysis of Calcite Structure using Fourier Transform Infra-Red (FTIR), and molecular identification using 16S rRNA gene sequences. Bacterial isolate SP. 48 were able to grow and produce calcite in both media, especially in tap water medium. FTIR results showed that the precipitates produced by bacterial isolates on both mediums had strong absorption peaks, which were detected to be calcite. Molecular identification using the 16S rRNA gene sequence showed that the isolate is Bacillus albus. B. albus is a proteolytic bacterium collected from landfills that was proven to be a calcite-producing bacterium, a new finding in this study. B. albus can grow and produce calcite in a tap water medium with low pH. This finding can be used as an alternative to overcome concrete cracks and increase the strength and durability of concrete. This bacterial isolate could be developed as a biocement candidate.
Co-Authors Berliansyah, Azizul Dedi Futra Defani, Syafiqah Amirah Delita Zul ERWINA JULIANTARI, ERWINA Monita Olivia Rahmani, Syauqi Susana Tetty Marta Linda Wati, Andini Saras