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All Journal JURNAL ILMIAH PLATAX TRITON : Jurnal Manajemen Sumberdaya Perairan Jurnal Abdi Insani Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Pakem : Jurnal Pengabdian Kepada Masyarakat Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Balobe: Jurnal Pengabdian Masyarakat Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan International Journal Sustainable Aquaculture
Matakupan, Jolen
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Processing Banana Peel Organic Waste in Tourism Areas as an Effort to Control Aquatic Environmental Pollution Tuhumury, Novianty; Sahetapy, Jacqueline M F; Matakupan, Jolen; Rijoly, Stefanno M A
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.55446

Abstract

This study aims to analyze the weight of waste, recycling potential, and processing of banana peel organic waste into eco enzyme as an effort to control environmental pollution. The research was conducted in August-November 2023 in the Pasar Minggu Beach tourist area, Ambon City. The data collection method used direct observation in the field. Banana peel waste data was collected for 4 days, then weighed for further processing into eco enzyme for 3 months. Waste generation data and recycling potential were calculated using the formula set by the National Standardization Agency. The results showed that the weight of banana peel waste generated was 32.2 kg with 195 visitors. The average waste generation obtained was 0.17 or 0.2 kg/person/day, meaning that every visitor who comes to the tourist attractions produces 0.2 kg of banana peel waste. Banana waste that can be recycled is 30 kg and produces 112.5 l of ready-to-use eco enzyme solution. Processing banana peel organic waste into eco enzyme solution contributes ecologically and economically positively. Keywords: Banana peel; Eco enzyme; Organic waste; Tourism; Waste generation. Abstrak Penelitian ini bertujuan untuk menganalisis berat timbulan, potensi daur ulang, serta pengolahan sampah organik kulit pisang menjadi eco enzyme sebagai upaya pengendalian pencemaran lingkungan. Penelitian dilakukan pada Agustus–November 2023 di kawasan wisata Pantai Pasar Minggu, Kota Ambon. Metode pengambilan data menggunakan metode observasi langsung di lapangan. Pengambilan data sampah kulit pisang dilakukan selama 4 hari, kemudian ditimbang beratnya untuk selanjutnya diolah menjadi eco enzyme selama 3 bulan. Data timbulan sampah dan potensi daur ulang dihitung merujuk pada rumus yang ditetapkan oleh Badan Standarisasi Nasional. Hasil penelitian menunjukan berat sampah kulit pisang yang dihasilkan sebesar 32,2 kg dengan jumlah pengunjung sebanyak 195 orang. Rata-rata timbulan sampah yang diperoleh sebesar 0,17 atau 0,2 kg/org/hr, artinya setiap pengunjung yang datang ke tempat wisata menghasilkan sampah kulit pisang sebesar 0,2 kg. Sampah pisang yang dapat didaur ulang sebesar 30 kg dan menghasilkan larutan eco enzyme siap pakai sebanyak 112,5 l. Pengolahan sampah organik kulit pisang menjadi larutan eco enzyme berkontribusi positif baik secara ekologis maupun ekonomis. Kata Kunci: Kulit pisang; Eco enzyme; Sampah organik; Wisata, Timbulan sampah
STUDI PARAMETER KUALITAS AIR BAGI KEGIATAN BUDIDAYA LOBSTER (Panulirus sp) DENGAN SISTEM KERAMBA JARING APUNG DI TELUK AMBON DALAM Louhenapessy, Daniel G; Matakupan, Jolen; Buton, D
TRITON: Jurnal Manajemen Sumberdaya Perairan Vol 19 No 2 (2023): TRITON: Jurnal Manajemen Sumberdaya Perairan
Publisher : Departement of Aquatic Resources Management, Fisheries and Marine Science Faculty, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/TRITONvol19issue2page114-121

Abstract

The demand for lobster on the market is experiencing an upward trend. The utilization of a floating net cage system is a viable opportunity for the cultivation of lobsters in the waters of Inner Ambon Bay. The objective of this study is to examine the water quality parameters associated with the cultivation of Lobster (Panulirus sp.) using a floating net cage system in the Inner Ambon Bay. Water quality parameters encompass a range of physical and chemical factors. Physical characteristics comprise of temperature, salinity, current, and brightness, while chemical parameters include pH, dissolved oxygen (DO), nitrite, nitrate, phosphate, and ammonia. The study was carried out in March 2023 within the seas of Inner Ambon Bay, utilizing a total of 21 designated observation locations. The field measurements encompassed the assessment of many parameters including temperature, salinity, current velocity, dissolved oxygen (DO) levels, pH, and brightness. Additional parameter measurements were conducted at the Maluku Province Health Laboratory Center. The data were analyzed in accordance with government regulations pertaining to the quality standards of sea water for marine biota. According to the findings of the research, the temperature measurements varied between 29 and 30.18°C, the salinity levels ranged from 33.39 - 34 ppt, the pH values spanned from 7 - 10.4, and the dissolved oxygen (DO) concentrations were observed to be between 4.27 and 7.12 milligrams per liter (mg/l). The obtained current velocity values ranged from 8.91 - 24.16 cm/sec, whereas the brightness value ranged from 4.5 - 10.5 m. For the concentration of chemical parameters, the results showed that phosphate ranged values from 0-98.81 mg/l, nitrate 0-0.02 mg/l, while nitrite and ammonia was 0 - 0.01 mg/l and 0 - 0.33 mg/l, respectively. The findings from the data analysis indicate that the overall physical and chemical characteristics of the water meet the established quality standards for marine organisms, particularly in relation to the suitability for sustaining lobsters (Panulirus sp) using a floating net cage system in Ambon Bay. ABSTRAK Permintaan pasar untuk komoditas lobster semakin meningkat. Perairan Teluk Ambon Dalam dapat dikembangkan untuk budidaya lobster dengan menggunakan sistem keramba jaring apung. Penelitian ini bertujuan untuk menganalisis paramater kualitas air bagi kegiatan budidaya Lobster (Panulirus sp) dengan sistem keramba jaring apung di Teluk Ambon Bagian Dalam. Parameter kualitas air meliputi parameter fisik (suhu, salinitas, arus, kecerahan) dan parameter kimia (pH, DO, nitrit, nitrat, fosfat, amonia). Penelitian ini dilakukan pada Maret 2023 di perairan Teluk Ambon Dalam pada 21 titik pengamatan. Parameter suhu, salinitas, arus, DO, pH dan kecerahan diukur secara langsung di lapangan. Sedangkan pengukuran parameter lainnya dilakukan pada Balai Laboratorium Kesehatan Provinsi Maluku. Analisa data menurujuk pada baku mutu air laut untuk biota laut sesuai peraturan pemerintah. Berdasarkan hasil penelitian diperoleh nilai suhu berkisar antara 29-30,18oC, salinitas 33,39- 34 ppt, pH 7-10,4, DO 4,27-7,12 mg/l. Nilai kecepatan arus diperoleh berkisar antara 8,91-24,16 cm/det, sedangkan nilai kecerahan sebesar 4,5-10,5 m. Parameter kimia meliputi fosfat berkisar antara 0,00-98,81 mg/l, nitrat 0,00-0,02 mg/l, nitrit 0,00-0,01 mg/l, dan amonia 0,00-0,33 mg/l. Hasil analisa data menunjukan bahwa parameter fisik dan kimia perairan secara keseluruhan memenuhi standar baku mutu biota laut, khususnya untuk pemeliharaan lobster (Panulirus sp) dengan sistem keramba jaring apung di perairan Teluk Ambon Dalam. Kata Kunci: Teluk Ambon Dalam, keramba jaring apung, lobster, Panulirus sp, kualitas air
AKTIVITAS ANTIBAKTERI ECO ENZYME TERHADAP BAKTERI YANG DIISOLASI DARI RUMPUT LAUT TERINFEKSI ICE-ICE Tuhumury, Novianty C; Sahetapy, Jacqueline M F; Matakupan, Jolen; Rijoly, Stefanno M A
TRITON: Jurnal Manajemen Sumberdaya Perairan Vol 20 No 1 (2024): TRITON: Jurnal Manajemen Sumberdaya Perairan
Publisher : Departement of Aquatic Resources Management, Fisheries and Marine Science Faculty, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/TRITONvol20issue1page54-61

Abstract

Eco enzyme is the result of fruit peel fermentation mixed with brown sugar water. Eco enzyme contains acetic acid that can inhibit the growth of bacteria, including bacteria isolated from seaweed infected with ice-ice disease. The purpose of this study was to analyze the antibacterial activity of eco enzyme to inhibit bacteria isolated from seaweed infected with ice-ice. This research was conducted in July-November 2023. The inhibition test was conducted at the BPBL Ambon Testing Laboratory, while the phytochemical test was conducted at the Herbal Materia Medica Laboratory in Batu, Malang. Eco enzyme was made at the Aquatic Resource Management Laboratory, Faculty of Fisheries and Marine Science, Pattimura University for three months. The method for antibacterial test of eco enzyme used disc diffusion method. There are four types of bacteria that have been isolated from ice-ice infected seaweed, namely Vibrio alginolyticus, Vibrio fluvalis, Vibrio cholerae, and Aeromonas cavie. There are four concentrations of eco enzyme used to inhibit the growth of isolated bacteria, namely 25%, 50%, 75% and 100%. Phytochemical test results showed that the eco enzyme solution contained phenol of 319.0921 mg GA/g, total flavonoids of 133.5000 mg Q/g, total tannins of 224.6916 mg ET/g, and IC50 value on DPPH (1,1-diphenyl-2-picrylhydrazil) method of 290.9558 ppm. Based on the results of the inhibition test, the greater the concentration of eco enzyme, the greater the inhibition zone produced. Inhibition zone with moderate category was found in bacteria V. alginolticus and A. cavie with 100% concentration, while others were included in the weak category. ABSTRAK Eco enzyme merupakan hasil fermentasi kulit buah dicampur dengan gula merah air. Eco enzyme mengandung asam asetat yang dapat menghambat pertumbuhan bakteri, antara lain bakteri yang diisolasi dari rumput laut yang terinfeksi penyakit ice-ice. Tujuan dari penelitian ini yaitu menganalisis aktivitas antibakteri eco enzyme untuk menghambat bakteri yang diisolasi dari rumput laut terinfeksi ice-ice. Penelitian ini dilakukan pada Juli-November 2023. Uji hambat dilakukan di Laboratorium Penguji BPBL Ambon, sedangkan untuk uji fitokimia dilakukan di Laboratorium Herbal Materia Medica Batu, Malang. Eco enzyme dibuat di Laboratorium MSP FPIK UNPATTI selama tiga bulan. Metode untuk uji antibakteri eco enzyme menggunakan metode difusi cakram. Terdapat empat jenis bakteri yang telah diisolasi dari rumput laut terinfeksi ice-ice yaitu Vibrio alginolyticus, Vibrio fluvalis, Vibrio cholerae, dan Aeromonas cavie. Terdapat empat konsentrasi eco enzyme yang digunakan untuk menghambat pertumbuhan bakteri isolasi yaitu 25%, 50%, 75% dan 100%. Hasil uji fitokimia menunjukan bahwa larutan eco enzyme mengandung Fenol sebesar 319,0921 mg GA/g, total Flavonoid sebesar 133,5000 mg Q/g, total Tanin sebesar 224,6916 mg ET/g, dan nilai IC50 pada metode DPPH (1,1-diphenil-2-picrylhydrazil) sebesar 290,9558 ppm. Berdasarkan hasil uji hambat diperoleh semakin besar konsentrasi eco enzyme maka semakin besar pula zona hambat yang dihasilkan. Zona hambat dengan kategori sedang ditemukan pada bakteri V. alginolticus dan A. cavie dengan konsentrasi 100%, sedangkan lainnya termasuk dalam kategori lemah. Kata Kunci: Eco enzyme, antibakteri, fitokimia, rumput laut, ice-ice
PENGENALAN METODE BUDIDAYA PERAIRAN PADA SISWA SEKOLAH MENENGAH ATAS Louhenapessy, Daniel George; Matakupan, Jolen
Balobe: Jurnal Pengabdian Masyarakat Vol 2 No 2 (2023): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.2.2.14-20

Abstract

Salah satu upaya yang dilakukan oleh pemerintah Kota Ambon adalah dengan meningkatkan peluang belajar ke tingkat pendidikan yang lebih tinggi untuk menghasilkan calon praktisi yang memilki penguasaan serta keterampilan di berbagai bidang yang salah satunya adalah budidaya perikanan. Hal yang sama juga yang hendak dicapai oleh Universitas Pattimura melalui Jurusan dan Program studi Budidaya Perairan pada Fakultas Perikanan dan Ilmu kelautan dengan melakukan Kegiatan Pengabdian kepada masyarakat. Kegiatan ini dilakukan dengan memperkenalkan potensi serta jenis sumberdaya yang ada di perairan Maluku, baik itu perairan laut, payau dan juga perairan tawar pada siswa SMA Negeri 8 Hutumuri Kota Ambon. Hasil yang ingin dicapai adalah dapat membuka wawasan siswa terhadap pengembangan kegiatan budidaya perikanan. Metode yang digunakan dalam kegiatan ini adalah melalui pemberian materi di kelas tentang metode atau teknik yang sesuai untuk kegiatan budidaya, serta faktor penting lain dalam sistem budidaya seperti pakan ikan. Kegiatan ini akan terus dilakukan secara periodik sehingga informasi mengenai teknologi dan perkembangan budidaya di Maluku akan menyentuh pada siswa-siswi di tingkat pendidikan menengah dan atas
Growth Of black sea cucumber Holothuria atra in an integrated aquaculture system Pattinasarany, Maureen Mercy; Palinussa, Elizabeth Miklen; Matakupan, Jolen
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.40045

Abstract

The black sea cucumber Holothuria atra was used in integrated aquaculture with sea bass juveniles (Lates calcarifer) as the lowest and highest trophic level organism, alongside the seaweed Gracilaria sp. as an organic extractive organism. Therefore, this study aimed to assess the potential of integrated aquaculture combined with sea bass juvenile and seaweed to support the growth of the black sea cucumber. The experiment was carried out using a completely randomized design with 3 fish density treatments (50, 100, and 150 fish/m3), each integrated with 10 bags of seaweed and 4 individual black sea cucumbers. The study was conducted for 8 weeks and the fish were fed with trash fish at 10% of body weight. Water quality parameters were measured in situ every day except for TOM, ammonia, and nitrate which were measured at the beginning, middle, and end of the study. The results showed that the specific growth rate of the black sea cucumber ranged from 0.31-0.45%/day. The black sea cucumber was able to use the feed and metabolic waste produced by the culture of sea bass, showing the highest growth rate at a density of 100 fish/m3. Water quality parameters remained in ranges considered suitable for growth, except salinity. Furthermore, the black sea cucumber was found to have a strategy for adapting to fluctuations in salinity, as shown by the ability to survive at 15 ppt.Keywords:Sea bass juvenilesSeaweedSalinityTOM
MANAJEMEN PENGELOLAAN IKAN KAKAP PUTIH DI KJA PADA SISWA SMA NEGERI 6 AMBON Palinussa, Elizabeth Miklen; Pattinasarany, Maurreen Mercy; Louhenapessy, Daniel George; Borut, Ruku Ratu; Sahetapy, Jacqueline M F; Matakupan, Jolen
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 2 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.2.159-164

Abstract

Ikan kakap putih merupakan jenis yang memiliki nilai jual yang tinggi dipelihara di keramba jaring apung. Manajemen pengelolaan ikan kakap putih menjadi alasan untuk harus diperhatikan agar mempersiapkan ikan yang berkualitas untuk dipasarkan. Tujuan dari kegiatan pengabdian ini adalah siswa dapat mengetahui dan memahami tentang manajemen dan pengelolaan ikan kakap putih yang dipelihara pada KJA. Kegiatan pengabdian kepada masyarakat berlangsung pada tanggal 6 Februari 2024. Yang bertempat di SMA Negeri 6 Kota Ambon. Metode yang diterapkan yaitu pembelajaran interaktif. Hasil yang diperoleh pada tes awal dengan nilai tertinggi (30,33%) dan nilai terendah yaitu (3.03%). Hal ini menggambarkan bawah tidak semua siswa memiliki pengetahuan awal yang sama sehingga materi ini sangat penting untuk diberikan untuk menambah pengetahuan siswa. Hasil akhir yang diperoleh nilai tertinggi (100%) dan nilai terendah yaitu (93,9%). Hal ini memperlihatkan bahwa adanya peningkatan pemahaman dan pengetahuan siswa setelah materi pemberian materi. Kesimpulan adanya peningkatan pengetahuan dan pemahaman siswa
Growth Of black sea cucumber Holothuria atra in an integrated aquaculture system Pattinasarany, Maureen Mercy; Palinussa, Elizabeth Miklen; Matakupan, Jolen
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.40045

Abstract

The black sea cucumber Holothuria atra was used in integrated aquaculture with sea bass juveniles (Lates calcarifer) as the lowest and highest trophic level organism, alongside the seaweed Gracilaria sp. as an organic extractive organism. Therefore, this study aimed to assess the potential of integrated aquaculture combined with sea bass juvenile and seaweed to support the growth of the black sea cucumber. The experiment was carried out using a completely randomized design with 3 fish density treatments (50, 100, and 150 fish/m3), each integrated with 10 bags of seaweed and 4 individual black sea cucumbers. The study was conducted for 8 weeks and the fish were fed with trash fish at 10% of body weight. Water quality parameters were measured in situ every day except for TOM, ammonia, and nitrate which were measured at the beginning, middle, and end of the study. The results showed that the specific growth rate of the black sea cucumber ranged from 0.31-0.45%/day. The black sea cucumber was able to use the feed and metabolic waste produced by the culture of sea bass, showing the highest growth rate at a density of 100 fish/m3. Water quality parameters remained in ranges considered suitable for growth, except salinity. Furthermore, the black sea cucumber was found to have a strategy for adapting to fluctuations in salinity, as shown by the ability to survive at 15 ppt.Keywords:Sea bass juvenilesSeaweedSalinityTOM
KAJIAN KEBERLANJUTAN USAHA BUDIDAYA RUMPUT LAUT Eucheuma cotonii DI PERAIRAN DUSUN WAEL KABUPATEN SERAM BAGIAN BARAT Matakupan, Jolen; Pattipeilohy, Christian E.; Tuhumury, Semuel F.; Sahetapy, Jacqueline M. F.; Louhenapessy, Daniel G.
Balobe: Jurnal Pengabdian Masyarakat Vol 4 No 1 (2025): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.4.1.43-54

Abstract

Seaweed is one of the leading types of aquaculture commodities with competitive market economic value in both domestic and export markets. This is partly because in addition to functioning as food, it is also due to the diversification of seaweed products that have. Wael Hamlet is one of the hamlets located in Piru Village, West Seram District. The majority of people in Wael Hamlet work as fishermen and cultivators, both fish farming and seaweed cultivation. Wael Hamlet is one of the E. Cottoni producing areas in West Seram Regency. The existing condition of seaweed cultivation in Wael Hamlet is currently experiencing a decline and even the fishermen do not produce seaweed. The conclusion of this activity is that the eucheuma cotonii seaweed cultivation business in the hamlet of Wael, West Seram Regency has decreased production due to disease attacks and pests in the form of baronagn fish and also turtles.
Distribusi Parameter Kualitas Air Di Teluk Kotania Untuk Evaluasi Kesesuaian Budidaya Rumput Laut Hatulesila, Greaty Ilona; Tuhumury, Semuel F.; Matakupan, Jolen
Juvenil Vol 6, No 3: Agustus (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i3.30388

Abstract

ABSTRAKTeluk Kotania di Kabupaten Seram Bagian Barat memiliki potensi besar untuk pengembangan budidaya rumput laut, namun keberlanjutannya sangat bergantung pada kualitas lingkungan perairan baik secara fisik, kimia, dan biologi. Penelitian ini bertujuan menganalisis parameter kualitas air yang mendukung budidaya rumput laut di wilayah tersebut. Pengambilan data dilakukan pada bulan Mei 2025 di 12 stasiun penelitian, meliputi parameter fisik, kimia, dan biologi melalui pengukuran in-situ dan uji laboratorium. Hasil penelitian menunjukkan bahwa sebagian besar parameter kualitas air seperti suhu (29,63–30,04 °C), salinitas (33,46–33,85 ppt), pH (7,06–7,65), kecerahan (6–18 m), oksigen terlarut (9,12–12,38 mg/l), COD (5,20–10,70 mg/l), dan BOD (3,40–8,70 mg/l) masih berada dalam kisaran optimal dan di bawah ambang batas baku mutu. Kecepatan arus tercatat antara 3,60–24,34 cm/detik, sementara kedalaman perairan berkisar antara 6–24 meter. Konsentrasi nitrat (0,015 mg/l) dan klorofil-a (0,18–3,73 µg/l) tergolong rendah, mengindikasikan kondisi perairan oligotrofik. Sebaliknya, ortofosfat tercatat cukup tinggi (0,0–0,18 mg/l) di beberapa lokasi, diduga akibat aktivitas antropogenik. Secara keseluruhan, hasil analisis menunjukkan bahwa Teluk Kotania masih layak untuk pengembangan budidaya rumput laut berdasarkan parameter kualitas air secara fisik, kimia, dan biologi.Kata Kunci: kualitas air, rumput laut, Teluk Kotania, budidaya lautABSTRACTKotania Bay in West Seram Regency has great potential for developing seaweed culture, but its sustainability is highly dependent on the quality of the aquatic environment, both physically, chemically, and biologically. This study aims to analyze water quality parameters that support seaweed culture in the area. Data was collected in May 2025 at 12 sampling stations, covering physical, chemical, and biological parameters through in-situ measurements and laboratory analysis. The results indicate that most water quality parameters such as temperature (29.63–30.04 °C), salinity (33.46–33.85 ppt), pH (7.06–7.65), transparency (6–18 m), dissolved oxygen (9.12–12.38 mg/l), COD (5.20–10.70 mg/l), and BOD (3.40–8.70 mg/l) are within optimal ranges and below the regulatory thresholds. Current velocity ranged from 3.60–24.34 cm/s, and water depth varied between 6–24 meters. Nitrate (0.015 mg/l) and chlorophyll-a (0.18–3.73 µg/l) levels were low, indicating oligotrophic conditions. Conversely, orthophosphate concentrations were relatively high (0.0–0.18 mg/l) at several locations, likely due to anthropogenic activities. Overall, the analysis suggests that Kotania Bay remains suitable for seaweed culture based on its physical, chemical, and biological water quality parameters.Keywords: water quality, seaweed, Kotania Bay, marine aquaculture
Evaluasi Konsentrasi Hormon Progresteron Pada Lobster (Panulirus versicolor) Dengan Ukuran Berbeda Menggunakan Metode Enzyme-Linked Immunosorbent Assay (ELISA) Matakupan, Jolen; Sahetapy, Jacqueline M. F.; Louhenapessy, Daniel G.; Luturmas, Absalom; Palinussa, Elizabeth M.
Indonesian Journal of Sustainable Aquaculture Vol 1 No 1 (2024): Indonesian Journal of Sustainable Aquaculture
Publisher : University of Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/aquaculture.1.1.28-35

Abstract

Control of gonad maturation and spawning is a major problem in the development of shrimp and lobster cultivation. Reproductive technology in hatching this type of crustacean has not experienced significant development. Progesterone is a precursor of estrogen in vertebrates, which is also found in crustaceans and other invertebrates. While several crustacean reproductive hormones have been characterized, the identity and function of other reproductive hormones are still under investigation. In hormone analysis, the use of the Enzyme Linked Immunosorbent Assay (ELISA) method continues to grow, both for research purposes and clinical applications. This study aims to evaluate the concentration of the hormone progesterone in Panulirus versicolor lobsters of different sizes with a body weight range of 300-750g which are caught in the wild using the ELISA method. The hormone progesterone is measured in the ovaries, hepatopancreas and hemolymph. This research uses descriptive analysis methods. From using the ELISA method, the results of the parallelism test showed that the test sample curve was parallel to the standard curve. Absorbance and standard value recovery tests showed that the measured concentrations of the progesterone hormone in Panulirus versicolor lobsters were acceptable because they had an average recovery value of 98%. The precision testing results of the ELISA kit used show that the test prescription is good and acceptable based on the percentage of coefficient variation (%CV) from QC1 and QC 2 (<15%). The concentration value of the progesterone hormone in P. versicolor lobsters shows that there is a difference in concentration depending on body size, where in lobsters with a body weight of 650-750g, the concentration of the progesterone hormone in the hepatopancreas, ovaries and hemolin is 1.059 ng/ml, 0.357 ng/ml and 0.042 ng/ml. while at lower body weights, namely 300-350 grams, the concentration of the hormone progesterone is 0.753 ng/ml, 0.181 ng/ml, 0.010 ng/ml.
Co-Authors Absalom Luturmas Buton, D Hatulesila, Greaty Ilona Louhenapessy, Daniel G Louhenapessy, Daniel G. Louhenapessy, Daniel George Louhenapessy, Danniel G. M.F Sahetapy, Jacqueline Palinussa, Elizabeth M. Palinussa, Elizabeth Miklen Pattinasarany, Maureen M Pattinasarany, Maurreen Mercy Pattipeilohy, Christian E. Pattipeilohy, Christian. E. Rijoly, Stefanno M A Ruku Ratu Borut Sahetapy, Jacqueline M. F Sahetapy, Jacqueline M. F. Tuhumury, Novianty Tuhumury, Novianty C Tuhumury, Semuel F.