Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BioEksakta : Jurnal Ilmiah Biologi Unsoed
Widyasari, Adristi Shafa
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Perkembangan Morfologi Serbuk Sari Passiflora vitifolia Kunth. Berdasarkan Tahap Antesis Bunga Widyasari, Adristi Shafa; Aryani, Riska Desi; Lestari, Sri; Palupi, Dian; Sukarsa, Sukarsa
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 4 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.4.10215

Abstract

Serbuk sari merupakan salah satu karakter khas dan unik yang dimiliki oleh setiap tumbuhan berbunga. Serbuk sari dapat dijadikan sebagai salah satu karakter dalam menentukan klasifikasi tumbuhan karena memiliki karakter morfologi berbeda pada setiap bunga dan pada tahap perkembangan bunganya (antesis). Proses perkembangan atau mekarnya bunga dapat berpengaruh pada perkembangan morfologi terhadap pembentukan serbuk sari. Penelitian ini bertujuan untuk mengetahui perkembangan morfologi serbuk sari Passiflora vitifolia Kunth. berdasarkan tahapan antesis bunga. Penelitian menggunakan serbuk sari dari 3 tahap antesis bunga: tahap 1 berupa kuncup ukuran 2 – 3 cm, tahap 2 berupa kuncup ukuran 5 – 8 cm dan tahap 3 bunga mekar sempurna. Hasil penelitian menunjukkan serbuk sari pada setiap tahap mekarnya bunga memiliki perbedaan karakter morfologi. Serbuk sari pada tahap 1 memiliki bentuk bikonkaf dengan warna hijau, sedangkan serbuk sari pada tahap 2 dan 3 memiliki bentuk bulat dengan warna coklat. Serbuk sari P. vitifolia memiliki tipe monad dan ornamentasi reticulate. Pada tahap 3 sebagian serbuk sari sudah terbentuk tabung serbuk sari (pollen tube).
INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Yusuf, Adib Fakhruddin; Latifah, Vida Rahma; Nurcahyati, Vivi Indah; Nurhasanah, Anggun Diyan; Widyasari, Adristi Shafa; Nainggolan, Ananto Puradi; Hasibuan, Aldy Riau Wansyah; Hidayat, Madyan Akmal; Karmilah, Karmilah; Pratiwi, Arini Dian; Aurantika, Rindu; Hasanah, Muslifah; Aristya, Ganies Riza; Handayani, Niken Satuti Nur; Arisuryanti, Tuty; Lesmana, Indra; Daryono, Budi Setiadi
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Co-Authors Aryani, Riska Desi Aurantika, Rindu BUDI SETIADI DARYONO Ganies Riza Aristya Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hidayat, Madyan Akmal Indra Lesmana Jayawarsa, A.A. Ketut Karmilah, Karmilah Latifah, Vida Rahma Nainggolan, Ananto Puradi Niken Satuti Nur Handayani Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Palupi, Dian Pratiwi, Arini Dian Sri Lestari Tuty Arisuryanti, Tuty Yusuf, Adib Fakhruddin