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All Journal Homes Journal: Hospital Management Studies Journal Jurnal Laboratorium Khatulistiwa Journal of Vocational Health Science
Evriarti, Paulina Rosa
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Identifikasi Bakteri Mirip Coliform pada Media Cromocoult Coliform Agar (CCA) Evriarti, Paulina Rosa
Jurnal Laboratorium Khatulistiwa Vol 6, No 1 (2022): November 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v6i1.1047

Abstract

Coliform-like bacteria can be found when examining coliforms in fresh water using the CCA media on membrane filter method. Therefore, it is necessary to characterize and identify coliform-like colonies that grow on CCA medium so that is easier to perform colony count analysis. One hundred ml of fresh water samples were filtered using filter membrane paper and then planted in CCA medium. Two different colonies growing on the media (red (A) and pink-purple (B)) were subjected to oxidase test, gram staining and re-cultured in various growth media such as blood agar, nutrient agar, MacConkey, and B. coli agar. Both colony types were also identified with Vitek MS to determine the species. The results of the examination showed that the red colonies (code A) were coliform-like bacteria, while the pink-purple colonies (code B) were coliform bacteria. The characteristics of coliform-like bacteria are that they produce hemolysis on blood agar and do not ferment lactose. Identification with Vitek MS showed that the isolated coliform-like bacteria was Aeromonas hydrophilla. Therefore, it can be concluded that Aeromonas sp. are coliform-like bacteria that grow on CCA, so further verification is needed when counting membrane filter colonies using CCA media.
DIAGNOSIS AND MANAGEMENT MOLECULER DETECTION OF BLOODSTREAM INFECTION Evriarti, Paulina Rosa; Wani Devita Gunardi
Homes Journal = Hospital Management Studies Journal Vol 5 No 2 (2024): Homes Journal: June
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/hmsj.v5i2.42765

Abstract

Examination for diagnosing sepsis or blood infections is conducted through blood culturing but this methode has several drawbacks such as a rather long incubation time. Therefore, the author has compiled this literature review to delve deeper into the potential and challenges of molecular diagnosis for blood infections or sepsis based on previous research findings. This Literature Review method was conducted using electronic databases including several databases such as Google Scholar, PubMed, and Semantic Schoolar. Base on the research, author get 8 journal that were discuccion in this journal. Molecular detection has potential function tn the future but research on the limitations of the method and optimization of PCR methods for detecting pathogens in the blood needs further investigation.
Perbedaan Serum dan Plasma EDTA yang Langsung Diperiksa dan Ditunda 1 dan 2 Jam Terhadap Penurunan Kadar Glukosa Darah Evriarti, Paulina Rosa; Lumban Toruan, Sari Artauli
Journal of Vocational Health Science Vol. 3 No. 2 (2024): Journal of Vocational Health Science (JOVAS)
Publisher : Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31884/jovas.v3i2.30

Abstract

Background: Serum and plasma are obtained after separating the liquid part of the blood cells through a centrifugation process. Before being separated, blood cells will use the glucose found in serum and plasma to maintain their viability. Purpose: Therefore, a study was conducted to determine the effect of delayed separation of serum and plasma on reducing glucose levels. Methods: Blood samples obtained from research subjects were divided into 2 groups (EDTA and without anticoagulants). Each group was divided into 3, for the 1st tube the serum and plasma were immediately separated, the 2nd tube was delayed 1 hour and the 3rd tube was delayed 2 hours. All samples were measured for glucose levels using the GOD-PAP method. Results: The data obtained was tested for its hypothesis with the Repeated Anova test (95% CI). The results showed that the mean serum and plasma glucose levels that were directly examined were 94.8 ± 7.3 mg/dl and 97.48 ± 7.8 mg/dL, which were delayed 1 hour 87.7 ± 7.6 and 92.81 ± 7.79 mg/dL and delayed 2 hours by 81.7 ± 8.03 and 89.3 ± 7.7 mg/dL. Statistical test results showed a significant value (p: 0.000) for differences in blood glucose levels between serum and plasma EDTA which were immediately separated and delayed 1 and 2 hours and p value: 0.059 for differences in serum and plasma glucose levels. Conclusion: It can be interpreted that serum and plasma EDTA glucose levels are not different, but the length of time the delay in separation of serum or plasma from blood cells affects the decrease in glucose levels.
Co-Authors Lumban Toruan, Sari Artauli Wani Devita Gunardi