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All Journal Jurnal Laboratorium Khatulistiwa Jurnal Info Kesehatan
Syopingi, Syopingi
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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UJI EFEKTIVITAS MINYAK ATSIRI DAUN KEMANGI (Ocimum sanctum Linnaeus) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Sutriswanto, Sutriswanto; Kurniawati, Fifi; Sulistiyowati, Endah; Syopingi, Syopingi
Jurnal Laboratorium Khatulistiwa Vol 7, No 1 (2023): NOVEMBER 2023
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i1.1245

Abstract

Herbal plants are plants that have a function in health as medicine. There are lots of plants that can be used as herbal medicine, one of which is the basil plant. Basil leaves contain chemical compounds including saponins, flavoniods, tannins and essential oils. The main ingredient in basil is essential oil. The essential oil of basil has the potential as an antibacterial. The purpose of this study was to analyze the effectiveness of basil leaf essential oil in inhibiting the growth of Staphylococcus aureus bacteria based on the standard antibiotic inhibition zone according to CLSI. This study used a quasi-experimental research design (Quasy Experiment) with a purposive sampling technique. The population of this research is basil (Ocimum sanctum Linnaeus). The samples used were basil essential oil concentrations of 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44% and 46%. Then tested the antibacterial activity using the diffusion method. Based on research the average inhibition zone at a concentration of 30% inhibition zone formed was 15 mm, 32% concentration was 18 mm, 34% concentration was 16.67 mm, 36% concentration was 15.67 mm, 38% concentration was 17.67 mm, 40% concentration was 22.33 mm, 42% concentration was 16.33 mm, 44% concentration was 18 mm, 46% concentration of 21.33 mm. The conclusion in this study is that there is effectiveness of basil leaf essential oil with the interpretation of Sensitive results in inhibiting the growth of Staphylococcus aureus bacteria by the diffusion method based on the standard antibiotic inhibition zone according to CLSI.
The potential of Cilembu sweet potato (Ipomoea batatas L.) as a growth medium for Staphylococcus aureus and Escherichia coli Nuswantoro, Ari; Rahayu, Merda Sri; Fatayati, Imma; Syopingi, Syopingi; Maretalinia, Maretalinia
JURNAL INFO KESEHATAN Vol 22 No 1 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss1.1087

Abstract

The media aims to store, reproduce, and identify bacteria but has disadvantages such as being expensive, containing chemicals, and being easily damaged due to contamination. Therefore we need an alternative media that can overcome these limitations. Cilembu sweet potato (Ipomoea batatas, L.) is a natural ingredient that is easy to obtain and contains sufficient nutrients so it has the potential to be used as a basic ingredient for growth media. The purpose of this study was to determine the potential of Cilembu sweet potato flour as an alternative medium for the growth of Staphylococcus aureus and Escherichia coli bacteria with a quasi-experimental design. Cilembu sweet potato flour is obtained by cleaning, chopping, drying in the oven, crushing with a blender, and sifting the tubers to obtain fairly fine flour. The flour was dissolved in agar and then inoculated with S. aureus and E. coli, each with 16 replications. The results showed that the average number of S. aureus colonies was 119.12 CFU (169.2 CFU in control) while E. coli was 160.56 CFU (221.2 CFU in control). The Mann-Whitney test showed that there was a difference in the number of S. aureus colonies on alternative media and NA (p = 0.006 ≤ 0.05), but there was no difference between the number of E. coli colonies on alternative media and NA (p = 0.057 > 0.05). Finally, there was a difference in the number of S. aureus and E. coli colonies on alternative media (p = 0.04 ≤ 0.05). The nutritional composition shows that Cilembu sweet potato flour has more potential to replace NA as a growth medium for E. coli than for S. aureus.
Co-Authors Ari Nuswantoro Imma Fatayati Kurniawati, Fifi Maretalinia, Maretalinia Rahayu, Merda Sri Sulistiyowati, Endah Sutriswanto, Sutriswanto